product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
laminin-1, avian
catalog :
3H11
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3H11
reactivity :
human, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 15
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; chicken; fig 5
Stanic K, Saldivia N, Förstera B, Torrejon M, Montecinos H, Caprile T. Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development. Front Neuroanat. 2016;10:89 pubmed
  • immunohistochemistry; chicken; fig 1
Atsuta Y, Takahashi Y. FGF8 coordinates tissue elongation and cell epithelialization during early kidney tubulogenesis. Development. 2015;142:2329-37 pubmed publisher
  • immunohistochemistry - paraffin section; chicken; 1:200; fig 1d
Murai H, Tadokoro R, Sakai K, Takahashi Y. In ovo gene manipulation of melanocytes and their adjacent keratinocytes during skin pigmentation of chicken embryos. Dev Growth Differ. 2015;57:232-41 pubmed publisher
  • immunocytochemistry; human; 1:400
Yoshino T, Saito D, Atsuta Y, Uchiyama C, Ueda S, Sekiguchi K, et al. Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet. Proc Natl Acad Sci U S A. 2014;111:6660-5 pubmed publisher
Yoshino T, Murai H, Saito D. Hedgehog-BMP signalling establishes dorsoventral patterning in lateral plate mesoderm to trigger gonadogenesis in chicken embryos. Nat Commun. 2016;7:12561 pubmed publisher
Cheng Y, Wang X, Wang G, Li Y, Chen Y, Chuai M, et al. Effects of Antitumor Drug Sorafenib on Chick Embryo Development. Anat Rec (Hoboken). 2015;298:1271-81 pubmed publisher
Halfter W, YIP J. An organizing function of basement membranes in the developing nervous system. Mech Dev. 2014;133:1-10 pubmed publisher
Nakaya Y, Sukowati E, Sheng G. Epiblast integrity requires CLASP and Dystroglycan-mediated microtubule anchoring to the basal cortex. J Cell Biol. 2013;202:637-51 pubmed publisher
Nakaya Y, Sukowati E, Wu Y, Sheng G. RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation. Nat Cell Biol. 2008;10:765-75 pubmed publisher
Candiello J, Balasubramani M, Schreiber E, Cole G, Mayer U, Halfter W, et al. Biomechanical properties of native basement membranes. FEBS J. 2007;274:2897-908 pubmed
Cao X, Pfaff S, Gage F. A functional study of miR-124 in the developing neural tube. Genes Dev. 2007;21:531-6 pubmed
Hummel S, Osanger A, Bajari T, Balasubramani M, Halfter W, Nimpf J, et al. Extracellular matrices of the avian ovarian follicle. Molecular characterization of chicken perlecan. J Biol Chem. 2004;279:23486-94 pubmed
Halfter W, von Boxberg Y. Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane. Eur J Neurosci. 1992;4:840-852 pubmed
Halfter W, Schurer B, Hasselhorn H, Christ B, Gimpel E, Epperlein H. An ovomucin-like protein on the surface of migrating primordial germ cells of the chick and rat. Development. 1996;122:915-23 pubmed
Halfter W. A heparan sulfate proteoglycan in developing avian axonal tracts. J Neurosci. 1993;13:2863-73 pubmed
product information
Internal ID :
4053
Name :
3H11
Depositor Name :
Halfter, W.M.
Depositor Institution :
University of Pittsburgh
Date Deposited :
2/8/01
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
laminin-1, avian
Antigen Species :
chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Avian
Species Tested (legacy) :
avian specific
Initial Publication Pubmed ID :
12106307
Depositor Notes (Special Instructions) :
This antibody is avian specific. Use non-reducing conditions for western blotting.
Collections :
Cell adhesion,Cell signaling,Extracellular matrix proteins
Search Keywords :
Halfter, W.M., laminin-1, avian, chicken, MIgG1, kappa light chain, Avian, LAMA1/LAMB1/LAMC1, , AB_528342, monoclonal, Cell signaling/Extracellular matrix proteins/Cell adhesion, Immunofluorescence/Western Blot
Antigen Molecular Weight :
Apparent: 200 kDa (beta1 and gamma1) and 400 kDa (alpha1)
Gene :
LAMA1/LAMB1/LAMC1
Uniprot ID :
F1NM47 Q01635 F1NI05
Antibody Registry ID :
AB_528342
Immunogen :
Retinal basal laminae (E10)
Immunogen Sequence :
full length protein
Clonality :
Monoclonal
Myeloma Strain :
X63Ag8.653
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Western Blot
Immunoblotting (legacy) :
Yes, non-reducing
Immunofluorescence Pubmed IDs :
12106307 8331377 17344415 15016830 18552836 8631269 25810088 27558761 25739909 27733818 26130757 24753584 25058486
Western Blot Pubmed IDs :
12106307 8331377 17488283
Additional Information :
This antibody recognizes native embryonic chicken laminin.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Neurosci. 13, 2863-2873.; Genes Dev. 21, 531-536.; Nat. Cell Biol. 10(7), 765-775.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US