Podocalyxin/gp135 | Developmental Studies Hybridoma Bank 3F2/D8 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Podocalyxin/gp135

catalog :

3F2/D8

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3F2/D8

reactivity :

human, dog

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; dog; 1:500; loading ...; fig 1e	BernabÃ© Rubio M, AndrÃ©s G, Casares Arias J, FernÃ¡ndez Barrera J, Rangel L, Reglero Real N, et al. Novel role for the midbody in primary ciliogenesis by polarized epithelial cells. J Cell Biol. 2016;214:259-73 pubmed publisher
western blot; human; fig 1	Klinkert K, Rocancourt M, Houdusse A, Echard A. Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening. Nat Commun. 2016;7:11166 pubmed publisher
	Gao L, Yang Z, Hiremath C, Zimmerman S, Long B, BRAKEMAN P, et al. Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017;144:3511-3520 pubmed publisher
	Toyoda Y, Takada T, Miyata H, Ishikawa T, Suzuki H. Regulation of the Axillary Osmidrosis-Associated ABCC11 Protein Stability by N-Linked Glycosylation: Effect of Glucose Condition. PLoS ONE. 2016;11:e0157172 pubmed publisher
	Fong ngern K, Peerapen P, Sinchaikul S, Chen S, Thongboonkerd V. Large-scale identification of calcium oxalate monohydrate crystal-binding proteins on apical membrane of distal renal tubular epithelial cells. J Proteome Res. 2011;10:4463-77 pubmed publisher
	Cheng H, Lin Y, Yu C, Chen J, Shen K, Lin W, et al. Molecular identification of canine podocalyxin-like protein 1 as a renal tubulogenic regulator. J Am Soc Nephrol. 2005;16:1612-22 pubmed
	Meder D, Shevchenko A, Simons K, Fullekrug J. Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells. J Cell Biol. 2005;168:303-13 pubmed
	Herzlinger D, Easton T, Ojakian G. The MDCK epithelial cell line expresses a cell surface antigen of the kidney distal tubule. J Cell Biol. 1982;93:269-77 pubmed
	Ojakian G, Schwimmer R. The polarized distribution of an apical cell surface glycoprotein is maintained by interactions with the cytoskeleton of Madin-Darby canine kidney cells. J Cell Biol. 1988;107:2377-87 pubmed
	Wang A, Ojakian G, Nelson W. Steps in the morphogenesis of a polarized epithelium. I. Uncoupling the roles of cell-cell and cell-substratum contact in establishing plasma membrane polarity in multicellular epithelial (MDCK) cysts. J Cell Sci. 1990;95 ( Pt 1):137-51 pubmed
	Ojakian G, Schwimmer R, Herz R. Polarized insertion of an intracellular glycoprotein pool into the apical membrane of MDCK cells. Am J Physiol. 1990;258:C390-8 pubmed

product information

Internal ID :

8635

Name :

3F2/D8

Depositor Name :

Ojakian, G.K.

Depositor Institution :

State University of New York, Brooklyn

Date Deposited :

6/27/13

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Podocalyxin/gp135

Antigen Species :

canine

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine

Species Tested (legacy) :

canine

Initial Publication Pubmed ID :

6178742

Depositor Notes (Special Instructions) :

The podocalyxin epitope withstands 4% paraformaldehyde/0.1% glutaraldehyde fixation but not paraffin processing.

Collections :

Cell adhesion,Cell markers

Search Keywords :

Ojakian, G.K., Podocalyxin/gp135, Canine, MIgG1, Canine, PODXL, PCLP,PCLP1 , , Monoclonal, Cell adhesion, Immunofluorescence/Western blot/Immunoprecipitation/ELISA/FFPE

Antigen Molecular Weight :

135 kDa

Gene :

PODXL

Alternate Gene Name(s) :

PCLP,PCLP1

Uniprot ID :

Q52S86

Antibody Registry ID :

AB_2618385

Immunogen :

Intact Madin-Darby canine kidney (MDCK) cells in adjuvant

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3U-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Extracellular N-terminus 59 a.a. following signal recognition peptide

Epitope Map PubMed ID :

15814834

Recommended Applications :

ELISA,FFPE,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

6178742 3198692 2316630 2351699 15642748 21859077

Western Blot Pubmed IDs :

6178742 3198692 15642748 21859077

Immunoprecipitation Pubmed IDs :

3198692

ELISA Pubmed IDs :

6178742 3198692 2316630

Additional Information :

RRID:AB_2618385

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 93(2), 269-277.; J. Cell Biol. 107(6 Pt 1), 2377-2387.; Am. J. Physiol. 258(3 Pt 1), C390-C398.; J. Cell Sci.95(1), 137-151.; J. Cell Sci.103(3), 677-687.; J. Cell Sci. 107 (3), 561-576.; J. Cell Biol. 168(2), 303-313.; Mol. Biol. Cell 18(5), 1710-1722.; Mol. Biol. Cell 20(1), 102-113.; J. Proteome. Res. 10(10), 4463-4477.; Am. J. Physiol. Renal Physiol. 300(6) F1375-F1384.

more info or order :

Developmental Studies Hybridoma Bank product webpage