product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Vimentin (radial glial and neuronal stem cells)
catalog :
3CB2
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3CB2
reactivity :
human, mouse, rat, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 9
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; chicken
Yan X, Lin J, Talabattula V, Mußmann C, Yang F, Wree A, et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS ONE. 2014;9:e84617 pubmed publisher
Daadi M, Grueter B, Malenka R, Redmond D, Steinberg G. Dopaminergic neurons from midbrain-specified human embryonic stem cell-derived neural stem cells engrafted in a monkey model of Parkinson's disease. PLoS ONE. 2012;7:e41120 pubmed publisher
Gupta K, Patani R, Baxter P, Serio A, Story D, Tsujita T, et al. Human embryonic stem cell derived astrocytes mediate non-cell-autonomous neuroprotection through endogenous and drug-induced mechanisms. Cell Death Differ. 2012;19:779-87 pubmed publisher
Kim H, Zahir T, Tator C, Shoichet M. Effects of dibutyryl cyclic-AMP on survival and neuronal differentiation of neural stem/progenitor cells transplanted into spinal cord injured rats. PLoS ONE. 2011;6:e21744 pubmed publisher
Keenan T, Nelson A, Grinager J, Thelen J, Svendsen C. Real time imaging of human progenitor neurogenesis. PLoS ONE. 2010;5:e13187 pubmed publisher
Daadi M, Maag A, Steinberg G. Adherent self-renewable human embryonic stem cell-derived neural stem cell line: functional engraftment in experimental stroke model. PLoS ONE. 2008;3:e1644 pubmed publisher
Strathmann F, Wang X, Mayer Proschel M. Identification of two novel glial-restricted cell populations in the embryonic telencephalon arising from unique origins. BMC Dev Biol. 2007;7:33 pubmed
Tanaka S, Miyamoto O, Janjua N, Miyazaki T, Takahashi F, Konishi R, et al. Stage and region dependent expression of a radial glial marker in commissural fibers in kindled mice. Epilepsy Res. 2005;67:61-72 pubmed
Talbott J, Loy D, Liu Y, Qiu M, Bunge M, Rao M, et al. Endogenous Nkx2.2+/Olig2+ oligodendrocyte precursor cells fail to remyelinate the demyelinated adult rat spinal cord in the absence of astrocytes. Exp Neurol. 2005;192:11-24 pubmed
product information
Internal ID :
2108
Name :
3CB2
Depositor Name :
De La Rosa, E.J.
Depositor Institution :
Centro de Investigaciones Biológicas CSIC, Madrid
Depositor Website :
http://www.vision-research.eu/index.php?id=598
Date Deposited :
2/13/96
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Vimentin (radial glial and neuronal stem cells)
Antigen Species :
Chick
Host Species :
mouse
Isotype :
MIgM
Isotype for catalog (legacy) :
IgM
Positive Tested Species Reactivity :
Chameleon,Chick,Human,Mouse,Quail,Rat
Species Tested (legacy) :
all tested (chick, rat, chameleon, human, mouse)
Initial Publication Pubmed ID :
8926034
Depositor Notes (Special Instructions) :
3CB2 is a cell marker from neural stem cells and radial glia. 3CB2 also recognizes three isoforms of vimentin in gliomas.
Collections :
Cell markers,Cytoskeleton,Human,Neurodevelopment
Search Keywords :
De La Rosa, E.J., Vimentin (radial glial and neuronal stem cells), Chick, MIgM, Chick/Rat/Human/Mouse/Chameleon/Quail, VIM, , AB_531888, Monoclonal, Human/Neurodevelopment/Cell Markers/Cytoskeleton, Western blot/Immunohistochemistry/Immunofluorescence
Antigen Molecular Weight :
53.1 kDa
Gene :
VIM
Uniprot ID :
P09654
Antibody Registry ID :
AB_531888
Immunogen :
E8 optic tectum, fractionated
Immunogen Sequence :
Full length sequence
Clonality :
Monoclonal
Myeloma Strain :
Ag8.653
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
positive
Immunohistochemistry Pubmed IDs :
8926034 18241054 12655601 15522889 15901663 16202564 22095276 22821702 24404179 29370163
Immunofluorescence Pubmed IDs :
8926034 18241054 12655601 15522889 15975939 16086633 17439658 17146440 19430532 19497285 19725137 20949053 21519547 21558414 22389700 22508027 27196425 28681861
Western Blot Pubmed IDs :
8926034 18241054 22508027
Additional Information :
RRID:AB_531888
DSHB Growth Medium :
Iscove's
References (legacy) :
Glia 15, 389-400.; Glia 42, 172-183.; Exp. Neurol. 192, 11-24.; Epilepsy Res. 67, 61-72.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US