product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Mmp1 catalytic domain
catalog :
3B8D12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3B8D12
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 30
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:10; loading ...; fig 7s2
Parvy J, Yu Y, Dostálová A, Kondo S, Kurjan A, Bulet P, et al. The antimicrobial peptide defensin cooperates with tumour necrosis factor to drive tumour cell death in Drosophila. elife. 2019;8: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:30; loading ...; fig f7s2e
Cosolo A, Jaiswal J, Csordás G, Grass I, Uhlirova M, Classen A. JNK-dependent cell cycle stalling in G2 promotes survival and senescence-like phenotypes in tissue stress. elife. 2019;8: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:10; loading ...; fig S1A
Raza Q, Vanderploeg J, Jacobs J. Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development. PLoS ONE. 2017;12:e0171905 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:100; loading ...; fig 3a
Bangi E, Murgia C, Teague A, Sansom O, Cagan R. Functional exploration of colorectal cancer genomes using Drosophila. Nat Commun. 2016;7:13615 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; fig 3
Levine B, Cagan R. Drosophila Lung Cancer Models Identify Trametinib plus Statin as Candidate Therapeutic. Cell Rep. 2016;14:1477-1487 pubmed publisher
Pinal N, Martin M, Medina I, Morata G. Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis. Nat Commun. 2018;9:1541 pubmed publisher
Aggarwal P, Gera J, Ghosh S, Mandal L, Mandal S. Noncanonical Decapentaplegic Signaling Activates Matrix Metalloproteinase 1 To Restrict Hedgehog Activity and Limit Ectopic Eye Differentiation in Drosophila. Genetics. 2017;207:197-213 pubmed publisher
Taylor E, Alqadri N, Dodgson L, Mason D, Lyulcheva E, Messina G, et al. MRL proteins cooperate with activated Ras in glia to drive distinct oncogenic outcomes. Oncogene. 2017;36:4311-4322 pubmed publisher
Toggweiler J, Willecke M, Basler K. The transcription factor Ets21C drives tumor growth by cooperating with AP-1. Sci Rep. 2016;6:34725 pubmed publisher
Takino K, Ohsawa S, Igaki T. Loss of Rab5 drives non-autonomous cell proliferation through TNF and Ras signaling in Drosophila. Dev Biol. 2014;395:19-28 pubmed publisher
de Vreede G, Schoenfeld J, Windler S, Morrison H, Lu H, Bilder D. The Scribble module regulates retromer-dependent endocytic trafficking during epithelial polarization. Development. 2014;141:2796-802 pubmed publisher
Herranz H, Weng R, Cohen S. Crosstalk between epithelial and mesenchymal tissues in tumorigenesis and imaginal disc development. Curr Biol. 2014;24:1476-84 pubmed publisher
Manhire Heath R, Golenkina S, Saint R, Murray M. Netrin-dependent downregulation of Frazzled/DCC is required for the dissociation of the peripodial epithelium in Drosophila. Nat Commun. 2013;4:2790 pubmed publisher
Nakajima Y, Meyer E, Kroesen A, McKinney S, Gibson M. Epithelial junctions maintain tissue architecture by directing planar spindle orientation. Nature. 2013;500:359-62 pubmed publisher
Fernandez B, Jezowska B, Janody F. Drosophila actin-Capping Protein limits JNK activation by the Src proto-oncogene. Oncogene. 2014;33:2027-39 pubmed publisher
Khoo P, Allan K, Willoughby L, Brumby A, Richardson H. In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling. Dis Model Mech. 2013;6:661-78 pubmed publisher
Chountala M, Vakaloglou K, Zervas C. Parvin overexpression uncovers tissue-specific genetic pathways and disrupts F-actin to induce apoptosis in the developing epithelia in Drosophila. PLoS ONE. 2012;7:e47355 pubmed publisher
Lee S, Park J, Kim Y, Chung H, Yoo M. Requirement of matrix metalloproteinase-1 for intestinal homeostasis in the adult Drosophila midgut. Exp Cell Res. 2012;318:670-81 pubmed publisher
Stevens L, Page McCaw A. A secreted MMP is required for reepithelialization during wound healing. Mol Biol Cell. 2012;23:1068-79 pubmed publisher
Robinson B, Moberg K. Drosophila endocytic neoplastic tumor suppressor genes regulate Sav/Wts/Hpo signaling and the c-Jun N-terminal kinase pathway. Cell Cycle. 2011;10:4110-8 pubmed publisher
Jezowska B, Fernández B, Amândio A, Duarte P, Mendes C, Bras Pereira C, et al. A dual function of Drosophila capping protein on DE-cadherin maintains epithelial integrity and prevents JNK-mediated apoptosis. Dev Biol. 2011;360:143-59 pubmed publisher
VanZomeren Dohm A, Sarro J, Flannery E, Duman Scheel M. The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor. BMC Dev Biol. 2011;11:41 pubmed publisher
Huang H, Du G, Chen H, Liang X, Li C, Zhu N, et al. Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus. Development. 2011;138:2477-85 pubmed publisher
Glasheen B, Robbins R, Piette C, Beitel G, Page McCaw A. A matrix metalloproteinase mediates airway remodeling in Drosophila. Dev Biol. 2010;344:772-83 pubmed publisher
Neisch A, Speck O, Stronach B, Fehon R. Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. J Cell Biol. 2010;189:311-23 pubmed publisher
Glasheen B, Kabra A, Page McCaw A. Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase. Proc Natl Acad Sci U S A. 2009;106:2659-64 pubmed publisher
Rodahl L, Haglund K, Sem Jacobsen C, Wendler F, Vincent J, Lindmo K, et al. Disruption of Vps4 and JNK function in Drosophila causes tumour growth. PLoS ONE. 2009;4:e4354 pubmed publisher
Miller C, Page McCaw A, Broihier H. Matrix metalloproteinases promote motor axon fasciculation in the Drosophila embryo. Development. 2008;135:95-109 pubmed
Zhang S, Dailey G, Kwan E, Glasheen B, Sroga G, Page McCaw A. An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion. Genes Dev. 2006;20:1899-910 pubmed publisher
Page McCaw A, Serano J, Sante J, Rubin G. Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development. Dev Cell. 2003;4:95-106 pubmed
product information
Internal ID :
1570
Name :
3B8D12
Depositor Name :
Rubin, G.M.
Depositor Institution :
HHMI / Janelia Farm Research
Date Deposited :
8/2/06
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Mmp1 catalytic domain
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
12530966
Depositor Notes (Special Instructions) :
This mAb is often used as a 1:1:1 cocktail when combined with 3A6B4 and 5H7B1.
Collections :
Drosophila antigens,Enzymes,Extracellular matrix proteins
Search Keywords :
Rubin, Gerald M., Mmp1 catalytic domain, Drosophila, MIgG1, kappa light chain, Drosophila, MMP1, dm1-MMP; Dm1-MMP; Dmel CG4859; dmmp1; dMMP1; , AB_579781, monoclonal
Antigen Molecular Weight :
Predicted: 64.7kDa; Apparent: multiple bands at 46, 52, 64 and 74kDa
Gene :
MMP1
Alternate Gene Name(s) :
dm1-MMP; Dm1-MMP; Dmel CG4859; dmmp1; dMMP1;
Uniprot ID :
Q9W122
Antibody Registry ID :
AB_579781
Immunogen :
GST fused to recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Immunogen Sequence :
Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Clonality :
Monoclonal
Myeloma Strain :
P3XAg8.653
Epitope Mapped :
Yes
Epitope Map PubMed ID :
12530966
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
18045838
Immunofluorescence Pubmed IDs :
16815999 12530966 23644660 20513443 23077599 24225841 22101275 23324326 21963538 19194501 21561986 23873041 22262460 25224221 22265916 21672235 20404112 24980505 29670104
Western Blot Pubmed IDs :
16815999 19196956 22262460
Immunoprecipitation Pubmed IDs :
16815999
Pubmed IDs :
25005475
Additional Information :
This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3A6B4 and 5H7B11.
DSHB Growth Medium :
Iscove's
References (legacy) :
Genes Dev. 20(14), 1899-1910.; Dev. Cell 4, 95-106.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US