product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Mmp1 catalytic domain
catalog :
3B8D12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3B8D12
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 30
Published Application/Species/Sample/Dilution | Reference |
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Miller C, Page McCaw A, Broihier H. Matrix metalloproteinases promote motor axon fasciculation in the Drosophila embryo. Development. 2008;135:95-109 pubmed
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Page McCaw A, Serano J, Sante J, Rubin G. Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development. Dev Cell. 2003;4:95-106 pubmed
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product information
Internal ID :
1570
Name :
3B8D12
Depositor Name :
Rubin, G.M.
Depositor Institution :
HHMI / Janelia Farm Research
Date Deposited :
8/2/06
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Mmp1 catalytic domain
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
12530966
Depositor Notes (Special Instructions) :
This mAb is often used as a 1:1:1 cocktail when combined with 3A6B4 and 5H7B1.
Collections :
Drosophila antigens,Enzymes,Extracellular matrix proteins
Search Keywords :
Rubin, Gerald M., Mmp1 catalytic domain, Drosophila, MIgG1, kappa light chain, Drosophila, MMP1, dm1-MMP; Dm1-MMP; Dmel CG4859; dmmp1; dMMP1; , AB_579781, monoclonal
Antigen Molecular Weight :
Predicted: 64.7kDa; Apparent: multiple bands at 46, 52, 64 and 74kDa
Gene :
MMP1
Alternate Gene Name(s) :
dm1-MMP; Dm1-MMP; Dmel CG4859; dmmp1; dMMP1;
Uniprot ID :
Q9W122
Antibody Registry ID :
AB_579781
Immunogen :
GST fused to recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Immunogen Sequence :
Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Clonality :
Monoclonal
Myeloma Strain :
P3XAg8.653
Epitope Mapped :
Yes
Epitope Map PubMed ID :
12530966
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
18045838
Immunofluorescence Pubmed IDs :
16815999 12530966 23644660 20513443 23077599 24225841 22101275 23324326 21963538 19194501 21561986 23873041 22262460 25224221 22265916 21672235 20404112 24980505 29670104
Western Blot Pubmed IDs :
16815999 19196956 22262460
Immunoprecipitation Pubmed IDs :
16815999
Pubmed IDs :
25005475
Additional Information :
This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3A6B4 and 5H7B11.
DSHB Growth Medium :
Iscove's
References (legacy) :
Genes Dev. 20(14), 1899-1910.; Dev. Cell 4, 95-106.
more info or order :
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Developmental Studies Hybridoma Bank
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