product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
collagen type III
catalog :
3B2
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3B2
reactivity :
chicken
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 12
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; chicken; 1:10; loading ...
Nagy N, Barad C, Graham H, Hotta R, Cheng L, Fejszak N, et al. Sonic hedgehog controls enteric nervous system development by patterning the extracellular matrix. Development. 2016;143:264-75 pubmed publisher
Alnahhas N, Le Bihan Duval E, Baéza E, Chabault M, Chartrin P, Bordeau T, et al. Impact of divergent selection for ultimate pH of pectoralis major muscle on biochemical, histological, and sensorial attributes of broiler meat. J Anim Sci. 2015;93:4524-31 pubmed publisher
Bódi I, Minkó K, Molnár D, Nagy N, Oláh I. A novel aspect of the structure of the avian thymic medulla. Cell Tissue Res. 2015;359:489-501 pubmed publisher
Biro E, Kocsis K, Nagy N, Molnar D, Kabell S, Palya V, et al. Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix. Avian Pathol. 2011;40:199-206 pubmed publisher
Nagy N, Mwizerwa O, Yaniv K, Carmel L, Pieretti Vanmarcke R, Weinstein B, et al. Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling. Dev Biol. 2009;330:263-72 pubmed publisher
Nagy N, Oláh I. Pyloric tonsil as a novel gut-associated lymphoepithelial organ of the chicken. J Anat. 2007;211:407-11 pubmed
Später D, Hill T, Gruber M, Hartmann C. Role of canonical Wnt-signalling in joint formation. Eur Cell Mater. 2006;12:71-80 pubmed
Hinton R, Lincoln J, Deutsch G, Osinska H, Manning P, Benson D, et al. Extracellular matrix remodeling and organization in developing and diseased aortic valves. Circ Res. 2006;98:1431-8 pubmed
Nagy N, Bíró E, Takacs A, Pólos M, Magyar A, Oláh I. Peripheral blood fibrocytes contribute to the formation of the avian spleen. Dev Dyn. 2005;232:55-66 pubmed
Swiderski R, Daniels K, Jensen K, Solursh M. Type II collagen is transiently expressed during avian cardiac valve morphogenesis. Dev Dyn. 1994;200:294-304 pubmed
Swasdison S, Mayne R. Location of the integrin complex and extracellular matrix molecules at the chicken myotendinous junction. Cell Tissue Res. 1989;257:537-43 pubmed
Swasdison S, Mayne R. Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo. J Cell Sci. 1992;102 ( Pt 3):643-52 pubmed
product information
Internal ID :
3419
Name :
3B2
Depositor Name :
Mayne, R.
Depositor Institution :
Univ. of Alabama at Birmingham
Date Deposited :
5/20/93
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
collagen type III
Antigen Species :
chicken
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Chicken
Species Tested (legacy) :
chicken only
Initial Publication Pubmed ID :
2529032
Depositor Notes (Special Instructions) :
3B2 appears to be chicken-specific and doesn't cross react with quail tissue or a variety of mammalian species.
Collections :
Extracellular matrix proteins
Search Keywords :
Mayne, R., collagen type III, Chicken, MIgG1, Chicken, COL3A1, , AB_528166, monoclonal, Extracellular matrix proteins, ELISA/Western Blot/Immunofluorescence/Immunohistochemistry
Antigen Molecular Weight :
Predicted: 78 kDa
Gene :
COL3A1
Uniprot ID :
P12105
Antibody Registry ID :
AB_528166
Immunogen :
Type III collagen from chicken gizzard.
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
P3X63Ag8.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
The epitope is located approximately one-fifth of the distance from the amino terminus of the collagen molecule (see reference in Additional Information).
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
15580570 19345201 21500040 25381569 26440351 17593219 16645142 15580570 17115376
Immunofluorescence Pubmed IDs :
1506438 7994076 15580570 15580570
Additional Information :
This antibody was originally described in Swasdison, S., Mayne, P.M., Wright, D.W., Accavitti, M.A., Fitch, J.M., Linsenmayer, T.F. and Mayne, R. (1992); Monoclonal antibodies that distinguish avian type I and type III collagens: Isolation, characterization and immunolocalization in various issues; Matrix 12, 56-65. ELISA and western blotting were performed in this article.
DSHB Growth Medium :
RPMI
References (legacy) :
Matrix 12, 56-65.; J. Cell Sci. 102, 643-652.; Dev. Dyn. 200, 294-304.; Dev. Dyn. 232, 55-66.; Dev. Biol. 330(2), 263-272.; Avian Pathol. 40(2), 199-206.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US