product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Spectrin, alpha
catalog :
3A9 (323 or M10-2)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3A9 (323 or M10-2)
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - free floating section
citations: 62
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:100; loading ...; fig 2s1i
Lattner J, Leng W, Knust E, Brankatschk M, Flores Benitez D. Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2 in Drosophila. elife. 2019;8: pubmed publisher
  • immunohistochemistry - paraffin section; Drosophila melanogaster; 1:200; loading ...; fig 7f
Ordonez D, Lee M, Feany M. α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton. Neuron. 2018;97:108-124.e6 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:25; loading ...; tbl 3
Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:25
Chen H, Huang Y, Pfeiffer B, Yao X, Lee T. An enhanced gene targeting toolkit for Drosophila: Golic+. Genetics. 2015;199:683-94 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:5
Furriols M, Casanova J. Germline and somatic vitelline proteins colocalize in aggregates in the follicular epithelium of Drosophila ovaries. Fly (Austin). 2014;8:113-9 pubmed publisher
  • immunocytochemistry; Drosophila melanogaster; 1:50
Chen D, Wu C, Zhao S, Geng Q, Gao Y, Li X, et al. Three RNA binding proteins form a complex to promote differentiation of germline stem cell lineage in Drosophila. PLoS Genet. 2014;10:e1004797 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:20; fig 1
Zacharogianni M, Aguilera Gomez A, Veenendaal T, Smout J, Rabouille C. A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation. elife. 2014;3: pubmed publisher
  • immunohistochemistry - free floating section; Drosophila melanogaster; 1:20
  • western blot; Drosophila melanogaster; 1:1000-10,000
Khanna M, Mattie F, Browder K, Radyk M, Crilly S, Bakerink K, et al. Spectrin tetramer formation is not required for viable development in Drosophila. J Biol Chem. 2015;290:706-15 pubmed publisher
Chandran R, Scholl A, Yang Y, Jiang L. rebuff regulates apical luminal matrix to control tube size in Drosophila trachea. Biol Open. 2018;7: pubmed publisher
Rotstein B, Post Y, Reinhardt M, Lammers K, Buhr A, Heinisch J, et al. Distinct domains in the matricellular protein Lonely heart are crucial for cardiac extracellular matrix formation and heart function in Drosophila. J Biol Chem. 2018;293:7864-7879 pubmed publisher
Lu K, Yamashita Y. Germ cell connectivity enhances cell death in response to DNA damage in the Drosophila testis. elife. 2017;6: pubmed publisher
He J, Xuan T, Xin T, An H, Wang J, Zhao G, et al. Evidence for chromatin-remodeling complex PBAP-controlled maintenance of the Drosophila ovarian germline stem cells. PLoS ONE. 2014;9:e103473 pubmed publisher
Loya C, McNeill E, Bao H, Zhang B, Van Vactor D. miR-8 controls synapse structure by repression of the actin regulator enabled. Development. 2014;141:1864-74 pubmed publisher
Driver I, Ohlstein B. Specification of regional intestinal stem cell identity during Drosophila metamorphosis. Development. 2014;141:1848-56 pubmed publisher
Sfregola M. Centralspindlin is required for thorax development during Drosophila metamorphosis. Genesis. 2014;52:387-98 pubmed publisher
Barton L, Wilmington S, Martin M, Skopec H, Lovander K, Pinto B, et al. Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila. Genetics. 2014;197:653-65 pubmed publisher
Torres I, Rosa Ferreira C, Munro S. The Arf family G protein Arl1 is required for secretory granule biogenesis in Drosophila. J Cell Sci. 2014;127:2151-60 pubmed publisher
Yan D, Neumüller R, BUCKNER M, Ayers K, Li H, Hu Y, et al. A regulatory network of Drosophila germline stem cell self-renewal. Dev Cell. 2014;28:459-73 pubmed publisher
Bätz T, Förster D, Luschnig S. The transmembrane protein Macroglobulin complement-related is essential for septate junction formation and epithelial barrier function in Drosophila. Development. 2014;141:899-908 pubmed publisher
Nishimura M, Kumsta C, Kaushik G, Diop S, Ding Y, Bisharat Kernizan J, et al. A dual role for integrin-linked kinase and ?1-integrin in modulating cardiac aging. Aging Cell. 2014;13:431-40 pubmed publisher
Joyce E, Apostolopoulos N, Beliveau B, Wu C. Germline progenitors escape the widespread phenomenon of homolog pairing during Drosophila development. PLoS Genet. 2013;9:e1004013 pubmed publisher
Shields A, Spence A, Yamashita Y, Davies E, Fuller M. The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells. Development. 2014;141:73-82 pubmed publisher
Sitaram P, Merkle J, Lee E, Lee L. asunder is required for dynein localization and dorsal fate determination during Drosophila oogenesis. Dev Biol. 2014;386:42-52 pubmed publisher
Lear B, Darrah E, Aldrich B, Gebre S, Scott R, Nash H, et al. UNC79 and UNC80, putative auxiliary subunits of the NARROW ABDOMEN ion channel, are indispensable for robust circadian locomotor rhythms in Drosophila. PLoS ONE. 2013;8:e78147 pubmed publisher
Giuliani G, Giuliani F, Volk T, Rabouille C. The Drosophila RNA-binding protein HOW controls the stability of dgrasp mRNA in the follicular epithelium. Nucleic Acids Res. 2014;42:1970-86 pubmed publisher
Lynch H, Crews S, Rosenthal B, Kim E, Gish R, Echiverri K, et al. Cellular mechanics of germ band retraction in Drosophila. Dev Biol. 2013;384:205-13 pubmed publisher
Xin T, Xuan T, Tan J, Li M, Zhao G, Li M. The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche. Dev Biol. 2013;384:1-12 pubmed publisher
Agrawal T, Sadaf S, Hasan G. A genetic RNAi screen for IP₃/Ca²⁺ coupled GPCRs in Drosophila identifies the PdfR as a regulator of insect flight. PLoS Genet. 2013;9:e1003849 pubmed publisher
Hudson A, Parrott B, Qian Y, Schulz C. A temporal signature of epidermal growth factor signaling regulates the differentiation of germline cells in testes of Drosophila melanogaster. PLoS ONE. 2013;8:e70678 pubmed publisher
Giuliani F, Giuliani G, Bauer R, Rabouille C. Innexin 3, a new gene required for dorsal closure in Drosophila embryo. PLoS ONE. 2013;8:e69212 pubmed publisher
Drechsler M, Schmidt A, Meyer H, Paululat A. The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity. PLoS Genet. 2013;9:e1003616 pubmed publisher
Barton L, Pinto B, Wallrath L, Geyer P. The Drosophila nuclear lamina protein otefin is required for germline stem cell survival. Dev Cell. 2013;25:645-54 pubmed publisher
Groth C, Sasamura T, Khanna M, Whitley M, Fortini M. Protein trafficking abnormalities in Drosophila tissues with impaired activity of the ZIP7 zinc transporter Catsup. Development. 2013;140:3018-27 pubmed publisher
Tögel M, Meyer H, Lehmacher C, Heinisch J, Pass G, Paululat A. The bHLH transcription factor hand is required for proper wing heart formation in Drosophila. Dev Biol. 2013;381:446-59 pubmed publisher
Lucas E, Khanal I, Gaspar P, Fletcher G, Polesello C, Tapon N, et al. The Hippo pathway polarizes the actin cytoskeleton during collective migration of Drosophila border cells. J Cell Biol. 2013;201:875-85 pubmed publisher
Xuan T, Xin T, He J, Tan J, Gao Y, Feng S, et al. dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary. Dev Biol. 2013;379:167-81 pubmed publisher
Lee H, Zhao N, Campion B, Nguyen M, Selleck S. Akt regulates glutamate receptor trafficking and postsynaptic membrane elaboration at the Drosophila neuromuscular junction. Dev Neurobiol. 2013;73:723-43 pubmed publisher
Papagiannouli F. The internal structure of embryonic gonads and testis development in Drosophila melanogaster requires scrib, lgl and dlg activity in the soma. Int J Dev Biol. 2013;57:25-34 pubmed publisher
Peters N, Thayer N, Kerr S, Tompa M, Berg C. Following the 'tracks': Tramtrack69 regulates epithelial tube expansion in the Drosophila ovary through Paxillin, Dynamin, and the homeobox protein Mirror. Dev Biol. 2013;378:154-69 pubmed publisher
Letizia A, Ricardo S, Moussian B, Martin N, Llimargas M. A functional role of the extracellular domain of Crumbs in cell architecture and apicobasal polarity. J Cell Sci. 2013;126:2157-63 pubmed publisher
Desai R, Sarpal R, Ishiyama N, Pellikka M, Ikura M, Tepass U. Monomeric ?-catenin links cadherin to the actin cytoskeleton. Nat Cell Biol. 2013;15:261-73 pubmed publisher
Zhao S, Chen D, Geng Q, Wang Z. The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila. Dev Biol. 2013;376:163-70 pubmed publisher
Kibanov M, Kotov A, Olenina L. Multicolor fluorescence imaging of whole-mount Drosophila testes for studying spermatogenesis. Anal Biochem. 2013;436:55-64 pubmed publisher
Chu D, Pan H, Wan P, Wu J, Luo J, Zhu H, et al. AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis. Development. 2012;139:3561-71 pubmed publisher
Kim Y, Bao H, Bonanno L, Zhang B, Serpe M. Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction. Genes Dev. 2012;26:974-87 pubmed publisher
Zhou B, Wu Y, Lin X. Retromer regulates apical-basal polarity through recycling Crumbs. Dev Biol. 2011;360:87-95 pubmed publisher
Baxley R, Soshnev A, Koryakov D, Zhimulev I, Geyer P. The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis. Dev Biol. 2011;356:398-410 pubmed publisher
Di Stefano L, Walker J, Burgio G, Corona D, Mulligan P, Naar A, et al. Functional antagonism between histone H3K4 demethylases in vivo. Genes Dev. 2011;25:17-28 pubmed publisher
Albrecht S, Altenhein B, Paululat A. The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster. Dev Biol. 2011;350:89-100 pubmed publisher
Kaplan N, Tolwinski N. Spatially defined Dsh-Lgl interaction contributes to directional tissue morphogenesis. J Cell Sci. 2010;123:3157-65 pubmed publisher
Siekhaus D, Haesemeyer M, Moffitt O, Lehmann R. RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila. Nat Cell Biol. 2010;12:605-10 pubmed publisher
LORENZO D, Li M, Mische S, Armbrust K, Ranum L, Hays T. Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila. J Cell Biol. 2010;189:143-58 pubmed publisher
Grusche F, Hidalgo C, Fletcher G, Sung H, Sahai E, Thompson B. Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]. BMC Dev Biol. 2009;9:14 pubmed publisher
Bogdanik L, Framery B, Frölich A, Franco B, Mornet D, Bockaert J, et al. Muscle dystroglycan organizes the postsynapse and regulates presynaptic neurotransmitter release at the Drosophila neuromuscular junction. PLoS ONE. 2008;3:e2084 pubmed publisher
Gorczyca D, Ashley J, Speese S, Gherbesi N, Thomas U, Gundelfinger E, et al. Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin. J Neurosci. 2007;27:1033-44 pubmed
Martin S, St Johnston D. A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity. Nature. 2003;421:379-84 pubmed
Schaefer M, Petronczki M, Dorner D, Forte M, Knoblich J. Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system. Cell. 2001;107:183-94 pubmed
Vied C, Horabin J. The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germline. Development. 2001;128:2649-60 pubmed
Dubreuil R, Maddux P, Grushko T, MacVicar G. Segregation of two spectrin isoforms: polarized membrane-binding sites direct polarized membrane skeleton assembly. Mol Biol Cell. 1997;8:1933-42 pubmed
Dubreuil R, Byers T, Branton D, Goldstein L, Kiehart D. Drosophilia spectrin. I. Characterization of the purified protein. J Cell Biol. 1987;105:2095-102 pubmed
Pesacreta T, Byers T, Dubreuil R, Kiehart D, Branton D. Drosophila spectrin: the membrane skeleton during embryogenesis. J Cell Biol. 1989;108:1697-709 pubmed
Dubreuil R, Byers T, Stewart C, Kiehart D. A beta-spectrin isoform from Drosophila (beta H) is similar in size to vertebrate dystrophin. J Cell Biol. 1990;111:1849-58 pubmed
product information
Internal ID :
1328
Name :
3A9 (323 or M10-2)
Depositor Name :
Branton, D. / Dubreuil, R.
Depositor Institution :
Biology, Harvard University
Date Deposited :
2/16/98
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Spectrin, alpha
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
3680372
Collections :
Cytoskeleton,Drosophila antigens
Search Keywords :
Branton, Daniel / Dubreuil, Ron, Spectrin, alpha, Drosophila, MIgG1, Drosophila, alpha-Spec, SPEC-A; alpha-spectrin; alpha-Spectrin; alphaSp; alphaSpec; alphaSpectrin; Spectrin, AB_528473, monoclonal
Antigen Molecular Weight :
Predicted: 278kDa; Apparent: 234kDa
Gene :
alpha-Spec
Alternate Gene Name(s) :
SPEC-A; alpha-spectrin; alpha-Spectrin; alphaSp; alphaSpec; alphaSpectrin; Spectrin
Uniprot ID :
P13395
Antibody Registry ID :
AB_528473
Immunogen :
Drosophila alpha spectrin-Trp E fusion protein produced in Escherichia coli
Immunogen Sequence :
Recombinant alpha spectrin, Partial protein
Clonality :
Monoclonal
Myeloma Strain :
Fox NY
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
9348534 11526072 20495554 21094637 24610947 24700821 24496626 24400780 24346697 24700158 24217913 24718988 18446215 24120347 19228425 21958744 11672526 12540903 20736316 17267557 23417122 21651900 24576427 23874219 24333177 25068272 24700509 23585349 23940622 23624310 23525000 24135149 23785054 23894431 23592328 23747982 23376537 23545328 24385920 23357237 23806619 23733343 28809158 29249285 29599288 30185423
Western Blot Pubmed IDs :
3680372 9348534 18446215 17267557 24098151
Immunoprecipitation Pubmed IDs :
2229176 20368622
Pubmed IDs :
2497103 21205864 24223770 22899846 22499592
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell Biol. 105, 2095-2102.; J. Cell Biol. 108, 1697-1709.; J. Cell Biol. 111, 1849-1858.; Mol. Biol. Cell 8, 1933-1942.; Development 128, 2649-2660.; Nat. Cell Biol. 12, 605-610.; Dev. Biol. 350, 89-100.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US