product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Mmp1 catalytic domain
catalog :
3A6B4
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3A6B4
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 29
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly ; 1:200; loading ...; fig 3c
Xu D, Wang L, Yamada K, Baena Lopez L. Non-apoptotic activation of Drosophila caspase-2/9 modulates JNK signaling, the tumor microenvironment, and growth of wound-like tumors. Cell Rep. 2022;39:110718 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; loading ...; fig 4b, 4d
Harris R, Stinchfield M, Nystrom S, Mckay D, Hariharan I. Damage-responsive, maturity-silenced enhancers regulate multiple genes that direct regeneration in Drosophila. elife. 2020;9: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:30; loading ...; fig f7s2e
Cosolo A, Jaiswal J, Csordás G, Grass I, Uhlirova M, Classen A. JNK-dependent cell cycle stalling in G2 promotes survival and senescence-like phenotypes in tissue stress. elife. 2019;8: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:10; loading ...; fig S1A
Raza Q, Vanderploeg J, Jacobs J. Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development. PLoS ONE. 2017;12:e0171905 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; fig 3
Ho D, Pallavi S, Artavanis Tsakonas S. The Notch-mediated hyperplasia circuitry in Drosophila reveals a Src-JNK signaling axis. elife. 2015;4:e05996 pubmed publisher
Gignac S, MacCharles K, Fu K, Bonaparte K, Akarsu G, Barrett T, et al. Mechanistic studies in Drosophila and chicken give new insights into functions of DVL1 in dominant Robinow syndrome. Dis Model Mech. 2023;16: pubmed publisher
Rambur A, Lours Calet C, Beaudoin C, Buñay J, Vialat M, Mirouse V, et al. Sequential Ras/MAPK and PI3K/AKT/mTOR pathways recruitment drives basal extrusion in the prostate-like gland of Drosophila. Nat Commun. 2020;11:2300 pubmed publisher
Nguyen T, Ralbovska A, Kugler J. RhoBTB Proteins Regulate the Hippo Pathway by Antagonizing Ubiquitination of LKB1. G3 (Bethesda). 2020;10:1319-1325 pubmed publisher
Thuma L, Carter D, Weavers H, Martin P. Drosophila immune cells extravasate from vessels to wounds using Tre1 GPCR and Rho signaling. J Cell Biol. 2018;217:3045-3056 pubmed publisher
Pinal N, Martin M, Medina I, Morata G. Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis. Nat Commun. 2018;9:1541 pubmed publisher
Paul M, Singh A, Dutta D, Mutsuddi M, Mukherjee A. Notch signals modulate lgl mediated tumorigenesis by the activation of JNK signaling. BMC Res Notes. 2018;11:247 pubmed publisher
Aggarwal P, Gera J, Ghosh S, Mandal L, Mandal S. Noncanonical Decapentaplegic Signaling Activates Matrix Metalloproteinase 1 To Restrict Hedgehog Activity and Limit Ectopic Eye Differentiation in Drosophila. Genetics. 2017;207:197-213 pubmed publisher
Taylor E, Alqadri N, Dodgson L, Mason D, Lyulcheva E, Messina G, et al. MRL proteins cooperate with activated Ras in glia to drive distinct oncogenic outcomes. Oncogene. 2017;36:4311-4322 pubmed publisher
Takino K, Ohsawa S, Igaki T. Loss of Rab5 drives non-autonomous cell proliferation through TNF and Ras signaling in Drosophila. Dev Biol. 2014;395:19-28 pubmed publisher
de Vreede G, Schoenfeld J, Windler S, Morrison H, Lu H, Bilder D. The Scribble module regulates retromer-dependent endocytic trafficking during epithelial polarization. Development. 2014;141:2796-802 pubmed publisher
Herranz H, Weng R, Cohen S. Crosstalk between epithelial and mesenchymal tissues in tumorigenesis and imaginal disc development. Curr Biol. 2014;24:1476-84 pubmed publisher
Khoo P, Allan K, Willoughby L, Brumby A, Richardson H. In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling. Dis Model Mech. 2013;6:661-78 pubmed publisher
Chountala M, Vakaloglou K, Zervas C. Parvin overexpression uncovers tissue-specific genetic pathways and disrupts F-actin to induce apoptosis in the developing epithelia in Drosophila. PLoS ONE. 2012;7:e47355 pubmed publisher
Pallavi S, Ho D, Hicks C, Miele L, Artavanis Tsakonas S. Notch and Mef2 synergize to promote proliferation and metastasis through JNK signal activation in Drosophila. EMBO J. 2012;31:2895-907 pubmed publisher
Lee S, Park J, Kim Y, Chung H, Yoo M. Requirement of matrix metalloproteinase-1 for intestinal homeostasis in the adult Drosophila midgut. Exp Cell Res. 2012;318:670-81 pubmed publisher
Stevens L, Page McCaw A. A secreted MMP is required for reepithelialization during wound healing. Mol Biol Cell. 2012;23:1068-79 pubmed publisher
Robinson B, Moberg K. Drosophila endocytic neoplastic tumor suppressor genes regulate Sav/Wts/Hpo signaling and the c-Jun N-terminal kinase pathway. Cell Cycle. 2011;10:4110-8 pubmed publisher
VanZomeren Dohm A, Sarro J, Flannery E, Duman Scheel M. The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor. BMC Dev Biol. 2011;11:41 pubmed publisher
Huang H, Du G, Chen H, Liang X, Li C, Zhu N, et al. Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus. Development. 2011;138:2477-85 pubmed publisher
Neisch A, Speck O, Stronach B, Fehon R. Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. J Cell Biol. 2010;189:311-23 pubmed publisher
Glasheen B, Kabra A, Page McCaw A. Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase. Proc Natl Acad Sci U S A. 2009;106:2659-64 pubmed publisher
Rodahl L, Haglund K, Sem Jacobsen C, Wendler F, Vincent J, Lindmo K, et al. Disruption of Vps4 and JNK function in Drosophila causes tumour growth. PLoS ONE. 2009;4:e4354 pubmed publisher
Zhang S, Dailey G, Kwan E, Glasheen B, Sroga G, Page McCaw A. An MMP liberates the Ninjurin A ectodomain to signal a loss of cell adhesion. Genes Dev. 2006;20:1899-910 pubmed publisher
Page McCaw A, Serano J, Sante J, Rubin G. Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development. Dev Cell. 2003;4:95-106 pubmed
product information
Internal ID :
1558
Name :
3A6B4
Depositor Name :
Rubin, G.M.
Depositor Institution :
HHMI / Janelia Farm Research
Date Deposited :
8/2/06
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Mmp1 catalytic domain
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
12530966
Collections :
Drosophila antigens,Enzymes,Extracellular matrix proteins
Search Keywords :
Rubin, Gerald M., Mmp1 catalytic domain, Drosophila, MIgG1, kappa light chain, Drosophila, MMP1, Dmel_CG4859; dm1-MMP; Dm1-MMP; Dmel CG4859; dmmp1; dMMP1; l(2)k04809; Mmp 1; MMP-1; mmp1; MMp1; MMP1, AB_579780, monoclonal
Antigen Molecular Weight :
Predicted: 65kDa; Apparent: multiple bands at 46, 52, 64 and 74kDa
Gene :
MMP1
Alternate Gene Name(s) :
Dmel_CG4859; dm1-MMP; Dm1-MMP; Dmel CG4859; dmmp1; dMMP1; l(2)k04809; Mmp 1; MMP-1; mmp1; MMp1; MMP1
Uniprot ID :
Q9W122
Antibody Registry ID :
AB_579780
Immunogen :
Mmp1 catalytic domain (amino acids 137-299 of Mmp1)-GST recombinant fusion protein
Immunogen Sequence :
Recombinant Mmp1 catalytic domain (amino acids 137-299 of Mmp1)
Clonality :
Monoclonal
Myeloma Strain :
P3XAg8.653
Epitope Mapped :
Yes
Epitope Map PubMed ID :
12530966
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
16815999 12530966 22580825 23077599 22101275 23324326 19194501 21561986 22262460 25224221 22265916 21672235 20404112 24980505 29670104 29661224
Western Blot Pubmed IDs :
16815999 19196956 22262460
Immunoprecipitation Pubmed IDs :
16815999
Pubmed IDs :
25005475
Additional Information :
This antibody has been used for immunostaining as a monoclonal antibody cocktail with 3B8D12 and 5H7B11.
DSHB Growth Medium :
Iscove's
References (legacy) :
Genes Dev. 20(14), 1899-1910.; Dev. Cell 4, 95-106.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US