product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Islet-1 & Islet-2 homeobox
catalog :
39.4D5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
39.4D5
reactivity :
human, mouse, rat, chicken, zebrafish , domestic sheep, Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, ChIP-Seq, western blot knockout validation
citations: 170
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:10; loading ...; fig s4b
  • ChIP-Seq; mouse; loading ...; fig 5b
  • chromatin immunoprecipitation; mouse; loading ...; fig s7c
  • immunoprecipitation; mouse; loading ...; fig 5k
Gao R, Liang X, Cheedipudi S, Cordero J, Jiang X, Zhang Q, et al. Pioneering function of Isl1 in the epigenetic control of cardiomyocyte cell fate. Cell Res. 2019;29:486-501 pubmed publisher
  • immunocytochemistry; human; 1:300; loading ...; fig 2b
Lee H, Lee H, Lee B, Gerovska D, Park S, Zaehres H, et al. Sequentially induced motor neurons from human fibroblasts facilitate locomotor recovery in a rodent spinal cord injury model. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; 1:100; loading ...; fig 1s1a
Kantarci H, Gou Y, Riley B. The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicle. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:20; loading ...; fig 3a
Hael C, Rojo D, Orquera D, Low M, Rubinstein M. The transcriptional regulator PRDM12 is critical for Pomc expression in the mouse hypothalamus and controlling food intake, adiposity, and body weight. Mol Metab. 2020;34:43-53 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1d
Suter T, Blagburn S, Fisher S, Anderson Keightly H, D Elia K, Jaworski A. TAG-1 Multifunctionality Coordinates Neuronal Migration, Axon Guidance, and Fasciculation. Cell Rep. 2020;30:1164-1177.e7 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:300; loading ...; fig s3d
Wan Y, Wei Z, Looger L, Koyama M, Druckmann S, Keller P. Single-Cell Reconstruction of Emerging Population Activity in an Entire Developing Circuit. Cell. 2019;179:355-372.e23 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 2f
Soldatov R, Kaucka M, Kastriti M, Petersen J, Chontorotzea T, Englmaier L, et al. Spatiotemporal structure of cell fate decisions in murine neural crest. Science. 2019;364: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 6f
Tulloch A, Teo S, Carvajal B, Tessier Lavigne M, Jaworski A. Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse. J Comp Neurol. 2019;527:2948-2972 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5d
Rajderkar S, Mann J, Panaretos C, Yumoto K, Li H, Mishina Y, et al. Trim33 is required for appropriate development of pre-cardiogenic mesoderm. Dev Biol. 2019;450:101-114 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 6f
Wizeman J, Guo Q, Wilion E, LI J. Specification of diverse cell types during early neurogenesis of the mouse cerebellum. elife. 2019;8: pubmed publisher
  • immunohistochemistry - frozen section; human; 1:20; loading ...; fig 6a
  • flow cytometry; human; 1:100; fig 7e
Sahara M, Santoro F, Sohlmér J, Zhou C, Witman N, Leung C, et al. Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract. Dev Cell. 2019;48:475-490.e7 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1a
Marcucci F, Soares C, Mason C. Distinct timing of neurogenesis of ipsilateral and contralateral retinal ganglion cells. J Comp Neurol. 2019;527:212-224 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:150; loading ...; fig 3w
Eley L, Alqahtani A, MacGrogan D, Richardson R, Murphy L, Salguero Jimenez A, et al. A novel source of arterial valve cells linked to bicuspid aortic valve without raphe in mice. elife. 2018;7: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2c
Wang B, Joo J, Mount R, Teubner B, Krenzer A, Ward A, et al. The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest. 2018;128:3319-3332 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 4c
Miesfeld J, Glaser T, Brown N. The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody. Gene Expr Patterns. 2018;27:114-121 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 6d
Tahara N, Akiyama R, Theisen J, Kawakami H, Wong J, Garry D, et al. Gata6 restricts Isl1 to the posterior of nascent hindlimb buds through Isl1 cis-regulatory modules. Dev Biol. 2018;434:74-83 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:100; loading ...; fig 1i
Tinterri A, Deck M, Keita M, Mailhes C, Rubin A, Kessaris N, et al. Tangential migration of corridor guidepost neurons contributes to anxiety circuits. J Comp Neurol. 2018;526:397-411 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2h
Tchieu J, Zimmer B, Fattahi F, Amin S, Zeltner N, Chen S, et al. A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages. Cell Stem Cell. 2017;21:399-410.e7 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 3e
Ohman Gault L, Huang T, KRIMM R. The transcription factor Phox2b distinguishes between oral and non-oral sensory neurons in the geniculate ganglion. J Comp Neurol. 2017;525:3935-3950 pubmed publisher
  • immunohistochemistry - free floating section; Xenopus laevis; 1:500; tbl 2
Moreno N, González A. Pattern of Neurogenesis and Identification of Neuronal Progenitor Subtypes during Pallial Development in Xenopus laevis. Front Neuroanat. 2017;11:24 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1b
Himmels P, Paredes I, Adler H, Karakatsani A, Luck R, Marti H, et al. Motor neurons control blood vessel patterning in the developing spinal cord. Nat Commun. 2017;8:14583 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig st4
Qi Y, Zhang X, Renier N, Wu Z, Atkin T, Sun Z, et al. Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells. Nat Biotechnol. 2017;35:154-163 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:10; loading ...; fig 1a
Witzel H, Cheedipudi S, Gao R, Stainier D, Dobreva G. Isl2b regulates anterior second heart field development in zebrafish. Sci Rep. 2017;7:41043 pubmed publisher
  • immunocytochemistry; mouse; fig 1b
Maltabe V, Barka E, Kontonika M, Florou D, Kouvara Pritsouli M, Roumpi M, et al. Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells Int. 2016;2016:8305624 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5e
Kahn B, Corman T, Lovelace K, Hong M, Krauss R, Epstein D. Prenatal ethanol exposure in mice phenocopies Cdon mutation by impeding Shh function in the etiology of optic nerve hypoplasia. Dis Model Mech. 2017;10:29-37 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s5k
Li Y, Tzatzalos E, Kwan K, Grumet M, Cai L. Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development. Sci Rep. 2016;6:38665 pubmed publisher
  • immunohistochemistry; mouse; 1:400; loading ...; fig s9
Espinosa Medina I, Saha O, Boismoreau F, Chettouh Z, Rossi F, Richardson W, et al. The sacral autonomic outflow is sympathetic. Science. 2016;354:893-897 pubmed
  • flow cytometry; human; 1:200; loading ...; fig s9c
Zeltner N, Fattahi F, Dubois N, Saurat N, Lafaille F, Shang L, et al. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia. Nat Med. 2016;22:1421-1427 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 5a
Junge H, Yung A, Goodrich L, Chen Z. Netrin1/DCC signaling promotes neuronal migration in the dorsal spinal cord. Neural Dev. 2016;11:19 pubmed
  • immunohistochemistry; mouse; fig 3s2
Leggere J, Saito Y, Darnell R, Tessier Lavigne M, Junge H, Chen Z. NOVA regulates Dcc alternative splicing during neuronal migration and axon guidance in the spinal cord. elife. 2016;5: pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 4
Baleriola J, Álvarez Lindo N, de la Villa P, Bernad A, Blanco L, Suárez T, et al. Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina. Sci Rep. 2016;6:25928 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 1a
Feng J, Xian Q, Guan T, Hu J, Wang M, Huang Y, et al. Celsr3 and Fzd3 Organize a Pioneer Neuron Scaffold to Steer Growing Thalamocortical Axons. Cereb Cortex. 2016;26:3323-34 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 6
Iwai Takekoshi L, Ramos A, Schaler A, Weinreb S, Blazeski R, Mason C. Retinal pigment epithelial integrity is compromised in the developing albino mouse retina. J Comp Neurol. 2016;524:3696-3716 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 1
  • immunohistochemistry; zebrafish ; 1:10; fig s4
Davey C, Mathewson A, Moens C. PCP Signaling between Migrating Neurons and their Planar-Polarized Neuroepithelial Environment Controls Filopodial Dynamics and Directional Migration. PLoS Genet. 2016;12:e1005934 pubmed publisher
  • immunocytochemistry; human; 1:100; fig s4
Fattahi F, Steinbeck J, Kriks S, Tchieu J, Zimmer B, Kishinevsky S, et al. Deriving human ENS lineages for cell therapy and drug discovery in Hirschsprung disease. Nature. 2016;531:105-9 pubmed publisher
  • immunocytochemistry; human; 1:500; tbl 1
de Souza C, Nivison Smith L, Christie D, Polkinghorne P, McGhee C, Kalloniatis M, et al. Macromolecular markers in normal human retina and applications to human retinal disease. Exp Eye Res. 2016;150:135-48 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250-1:500; fig 1
Gayet Primo J, Puthussery T. Alterations in Kainate Receptor and TRPM1 Localization in Bipolar Cells after Retinal Photoreceptor Degeneration. Front Cell Neurosci. 2015;9:486 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:5000; loading ...; tbl 1
Pirson M, Debrulle S, Clippe A, Clotman F, Knoops B. Thioredoxin-2 Modulates Neuronal Programmed Cell Death in the Embryonic Chick Spinal Cord in Basal and Target-Deprived Conditions. PLoS ONE. 2015;10:e0142280 pubmed publisher
  • immunohistochemistry; human; fig 5d
  • immunoprecipitation; mouse; fig 1c
  • immunohistochemistry; mouse; 1:1000; fig 2
  • western blot; mouse; 1:1000; fig 1a
Galloway J, Bethea M, Liu Y, Underwood R, Mobley J, Hunter C. SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic β-Cell Target Genes. Mol Endocrinol. 2015;29:1774-86 pubmed publisher
  • immunohistochemistry; mouse; fig 6
Hua Z, Emiliani F, Nathans J. Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems. Neural Dev. 2015;10:21 pubmed publisher
  • immunohistochemistry; mouse; 1:50
Huettl R, Eckstein S, Stahl T, Petricca S, Ninkovic J, Götz M, et al. Functional dissection of the Pax6 paired domain: Roles in neural tube patterning and peripheral nervous system development. Dev Biol. 2016;413:86-103 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 5
Lenzi J, De Santis R, de Turris V, Morlando M, Laneve P, Calvo A, et al. ALS mutant FUS proteins are recruited into stress granules in induced pluripotent stem cell-derived motoneurons. Dis Model Mech. 2015;8:755-66 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 1j
Watanabe S, Sanuki R, Sugita Y, Imai W, Yamazaki R, Kozuka T, et al. Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity. J Neurosci. 2015;35:8004-20 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 2
Adams K, Rousso D, Umbach J, Novitch B. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun. 2015;6:6778 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:20; fig 1
Holzmann J, Hennchen M, Rohrer H. Prox1 identifies proliferating neuroblasts and nascent neurons during neurogenesis in sympathetic ganglia. Dev Neurobiol. 2015;75:1352-67 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 5
Maury Y, Côme J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 5a
Zou M, Luo H, Xiang M. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn. 2015;244:86-97 pubmed publisher
  • immunohistochemistry; mouse; 1:500
Whitney I, Keeley P, St John A, Kautzman A, Kay J, Reese B. Sox2 regulates cholinergic amacrine cell positioning and dendritic stratification in the retina. J Neurosci. 2014;34:10109-21 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000
  • chromatin immunoprecipitation; mouse
  • EMSA; mouse
Ediger B, Du A, Liu J, Hunter C, Walp E, Schug J, et al. Islet-1 Is essential for pancreatic ?-cell function. Diabetes. 2014;63:4206-17 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:20
  • immunohistochemistry; chicken; 1:20
Lee K, Seo J, Shin J, Ji E, Roh J, Kim J, et al. Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system. Proc Natl Acad Sci U S A. 2014;111:2794-9 pubmed publisher
  • immunohistochemistry - free floating section; Xenopus laevis; 1:500
Dominguez L, González A, Moreno N. Characterization of the hypothalamus of Xenopus laevis during development. II. The basal regions. J Comp Neurol. 2014;522:1102-31 pubmed publisher
  • immunocytochemistry; domestic sheep; fig 5
Weber B, Kehl D, Bleul U, Behr L, Sammut S, Frese L, et al. In vitro fabrication of autologous living tissue-engineered vascular grafts based on prenatally harvested ovine amniotic fluid-derived stem cells. J Tissue Eng Regen Med. 2016;10:52-70 pubmed publisher
  • immunohistochemistry; mouse; fig 2
Le Bras B, Fréal A, Czarnecki A, Legendre P, Bullier E, Komada M, et al. In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014;219:1433-50 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:200
Nivison Smith L, Sun D, Fletcher E, Marc R, Kalloniatis M. Mapping kainate activation of inner neurons in the rat retina. J Comp Neurol. 2013;521:2416-38 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Favero C, Henshaw R, Grimsley Myers C, Shrestha A, Beier D, Dwyer N. Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain. J Comp Neurol. 2013;521:677-96 pubmed publisher
Chhabra S, Liu L, Goh R, Kong X, Warmflash A. Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids. PLoS Biol. 2019;17:e3000498 pubmed publisher
Dong X, Xie X, Guo L, Xu J, Xu M, Liang G, et al. Generation and characterization of Lhx4tdT reporter knock-in and Lhx4loxP conditional knockout mice. Genesis. 2019;57:e23328 pubmed publisher
Schick E, McCaffery S, Keblish E, Thakurdin C, Emerson M. Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina. Sci Rep. 2019;9:9358 pubmed publisher
De Santis R, Alfano V, de Turris V, Colantoni A, Santini L, Garone M, et al. Mutant FUS and ELAVL4 (HuD) Aberrant Crosstalk in Amyotrophic Lateral Sclerosis. Cell Rep. 2019;27:3818-3831.e5 pubmed publisher
Murcia Belmonte V, Coca Y, Vegar C, Negueruela S, de Juan Romero C, Valiño A, et al. A Retino-retinal Projection Guided by Unc5c Emerged in Species with Retinal Waves. Curr Biol. 2019;29:1149-1160.e4 pubmed publisher
Jacquier A, Risson V, Schaeffer L. Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons. J Vis Exp. 2019;: pubmed publisher
Jia G, Preussner J, Chen X, Guenther S, Yuan X, Yekelchyk M, et al. Single cell RNA-seq and ATAC-seq analysis of cardiac progenitor cell transition states and lineage settlement. Nat Commun. 2018;9:4877 pubmed publisher
Yen Y, Hsieh W, Tsai Y, Lü Y, Liau E, Hsu H, et al. Dlk1-Dio3 locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity. elife. 2018;7: pubmed publisher
Pane L, Fulcoli F, Cirino A, Altomonte A, Ferrentino R, Bilio M, et al. Tbx1 represses Mef2c gene expression and is correlated with histone 3 deacetylation of the anterior heart field enhancer. Dis Model Mech. 2018;11: pubmed publisher
Ghosh P, Maurer J, SAGERSTROM C. Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish. Neural Dev. 2018;13:13 pubmed publisher
Washausen S, Knabe W. Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals. Biol Open. 2018;7: pubmed publisher
Ray T, Roy S, Kozlowski C, Wang J, Cafaro J, Hulbert S, et al. Formation of retinal direction-selective circuitry initiated by starburst amacrine cell homotypic contact. elife. 2018;7: pubmed publisher
Maurer J, SAGERSTROM C. A parental requirement for dual-specificity phosphatase 6 in zebrafish. BMC Dev Biol. 2018;18:6 pubmed publisher
Capauto D, Colantoni A, Lu L, Santini T, Peruzzi G, Biscarini S, et al. A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons. Mol Neurobiol. 2018;55:7635-7651 pubmed publisher
Gou Y, Vemaraju S, Sweet E, Kwon H, Riley B. sox2 and sox3 Play unique roles in development of hair cells and neurons in the zebrafish inner ear. Dev Biol. 2018;435:73-83 pubmed publisher
Boubakar L, Falk J, Ducuing H, Thoinet K, Reynaud F, Derrington E, et al. Molecular Memory of Morphologies by Septins during Neuron Generation Allows Early Polarity Inheritance. Neuron. 2017;95:834-851.e5 pubmed publisher
Huang C, Lien C, Cheng C, Yen T, Chen C, Tsaur M. K+ Channel Kv3.4 Is Essential for Axon Growth by Limiting the Influx of Ca2+ into Growth Cones. J Neurosci. 2017;37:4433-4449 pubmed publisher
Park K, Randazzo G, Jones K, Brzezinski J. Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina. Invest Ophthalmol Vis Sci. 2017;58:1137-1150 pubmed publisher
Dvorakova M, Jahan I, Macova I, Chumak T, Bohuslavova R, Syka J, et al. Incomplete and delayed Sox2 deletion defines residual ear neurosensory development and maintenance. Sci Rep. 2016;6:38253 pubmed publisher
Ranga A, Girgin M, Meinhardt A, Eberle D, Caiazzo M, Tanaka E, et al. Neural tube morphogenesis in synthetic 3D microenvironments. Proc Natl Acad Sci U S A. 2016;113:E6831-E6839 pubmed
Saller M, Huettl R, Hanuschick P, Amend A, Alberton P, Aszodi A, et al. The role of Sema3-Npn-1 signaling during diaphragm innervation and muscle development. J Cell Sci. 2016;129:3295-308 pubmed publisher
Johnson K, Barragan J, Bashiruddin S, Smith C, Tyrrell C, Parsons M, et al. Gfap-positive radial glial cells are an essential progenitor population for later-born neurons and glia in the zebrafish spinal cord. Glia. 2016;64:1170-89 pubmed publisher
Bohuslavova R, Dodd N, Macova I, Chumak T, Horak M, Syka J, et al. Pax2-Islet1 Transgenic Mice Are Hyperactive and Have Altered Cerebellar Foliation. Mol Neurobiol. 2017;54:1352-1368 pubmed publisher
Shinmyo Y, Asrafuzzaman Riyadh M, Ahmed G, Bin Naser I, Hossain M, Takebayashi H, et al. Draxin from neocortical neurons controls the guidance of thalamocortical projections into the neocortex. Nat Commun. 2015;6:10232 pubmed publisher
Sato S, Yajima H, Furuta Y, Ikeda K, Kawakami K. Activation of Six1 Expression in Vertebrate Sensory Neurons. PLoS ONE. 2015;10:e0136666 pubmed publisher
Tomas Roca L, Tsaalbi Shtylik A, Jansen J, Singh M, Epstein J, Altunoglu U, et al. De novo mutations in PLXND1 and REV3L cause Möbius syndrome. Nat Commun. 2015;6:7199 pubmed publisher
Jarrar W, Dias J, Ericson J, Arnold H, Holz A. Nkx2.2 and Nkx2.9 are the key regulators to determine cell fate of branchial and visceral motor neurons in caudal hindbrain. PLoS ONE. 2015;10:e0124408 pubmed publisher
Masuda T, Sakuma C, Yaginuma H, Taniguchi M. Attractive and permissive activities of semaphorin 5A toward dorsal root ganglion axons in higher vertebrate embryos. Cell Adh Migr. 2014;8:603-6 pubmed publisher
Tillo M, Erskine L, Cariboni A, Fantin A, Joyce A, Denti L, et al. VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain. Development. 2015;142:314-9 pubmed publisher
Serinagaoglu Y, Paré J, Giovannini M, Cao X. Nf2-Yap signaling controls the expansion of DRG progenitors and glia during DRG development. Dev Biol. 2015;398:97-109 pubmed publisher
Vicario A, Abellán A, Desfilis E, Medina L. Genetic identification of the central nucleus and other components of the central extended amygdala in chicken during development. Front Neuroanat. 2014;8:90 pubmed publisher
Yajima H, Suzuki M, Ochi H, Ikeda K, Sato S, Yamamura K, et al. Six1 is a key regulator of the developmental and evolutionary architecture of sensory neurons in craniates. BMC Biol. 2014;12:40 pubmed publisher
Jacob C, Lötscher P, Engler S, Baggiolini A, Varum Tavares S, Brügger V, et al. HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia. J Neurosci. 2014;34:6112-22 pubmed publisher
Lin J, Wang C, Redies C. Restricted expression of classic cadherins in the spinal cord of the chicken embryo. Front Neuroanat. 2014;8:18 pubmed publisher
Deng M, Yang H, Xie X, Liang G, Gan L. Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. Gene Expr Patterns. 2014;15:31-7 pubmed publisher
Li Y, Pan J, Wei C, Chen J, Liu Y, Liu J, et al. LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3. BMC Biol. 2014;12:25 pubmed publisher
Li R, Wu F, Ruonala R, Sapkota D, Hu Z, Mu X. Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells. PLoS ONE. 2014;9:e92105 pubmed publisher
Pavlou S, Astell K, Kasioulis I, Gakovic M, Baldock R, van Heyningen V, et al. Pleiotropic effects of Sox2 during the development of the zebrafish epithalamus. PLoS ONE. 2014;9:e87546 pubmed publisher
Hua Z, Smallwood P, Nathans J. Frizzled3 controls axonal development in distinct populations of cranial and spinal motor neurons. elife. 2013;2:e01482 pubmed publisher
Nakamoto C, Kuan S, Findlay A, Durward E, Ouyang Z, Zakrzewska E, et al. Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development. Mol Biol Cell. 2014;25:234-44 pubmed publisher
Shi M, Kumar S, Motajo O, Kretschmer F, Mu X, Badea T. Genetic interactions between Brn3 transcription factors in retinal ganglion cell type specification. PLoS ONE. 2013;8:e76347 pubmed publisher
Becker S, Singhal S, Jones M, Eastlake K, Cottrill P, Jayaram H, et al. Acquisition of RGC phenotype in human Müller glia with stem cell characteristics is accompanied by upregulation of functional nicotinic acetylcholine receptors. Mol Vis. 2013;19:1925-36 pubmed
Wilfinger A, Arkhipova V, Meyer D. Cell type and tissue specific function of islet genes in zebrafish pancreas development. Dev Biol. 2013;378:25-37 pubmed publisher
Chou H, Lai D, Huang C, McLennan I, Wang H, Wang P. BMP4 is a peripherally-derived factor for motor neurons and attenuates glutamate-induced excitotoxicity in vitro. PLoS ONE. 2013;8:e58441 pubmed publisher
Hoffmann S, Berger I, Glaser A, Bacon C, Li L, Gretz N, et al. Islet1 is a direct transcriptional target of the homeodomain transcription factor Shox2 and rescues the Shox2-mediated bradycardia. Basic Res Cardiol. 2013;108:339 pubmed publisher
Zhang T, Liu J, Zhang J, Thekkethottiyil E, Macatee T, Ismat F, et al. Jun is required in Isl1-expressing progenitor cells for cardiovascular development. PLoS ONE. 2013;8:e57032 pubmed publisher
Di Bonito M, Narita Y, Avallone B, Sequino L, Mancuso M, Andolfi G, et al. Assembly of the auditory circuitry by a Hox genetic network in the mouse brainstem. PLoS Genet. 2013;9:e1003249 pubmed publisher
Willaredt M, Gorgas K, Gardner H, Tucker K. Multiple essential roles for primary cilia in heart development. Cilia. 2012;1:23 pubmed publisher
Hunter C, Dixit S, Cohen T, Ediger B, Wilcox C, Ferreira M, et al. Islet ?-, ?-, and ?-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor. Diabetes. 2013;62:875-86 pubmed publisher
Vemaraju S, Kantarci H, Padanad M, Riley B. A spatial and temporal gradient of Fgf differentially regulates distinct stages of neural development in the zebrafish inner ear. PLoS Genet. 2012;8:e1003068 pubmed publisher
Seredick S, Van Ryswyk L, Hutchinson S, Eisen J. Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics. Neural Dev. 2012;7:35 pubmed publisher
Tessadori F, van Weerd J, Burkhard S, Verkerk A, de Pater E, Boukens B, et al. Identification and functional characterization of cardiac pacemaker cells in zebrafish. PLoS ONE. 2012;7:e47644 pubmed publisher
Miyazaki S, Tashiro F, Fujikura J, Yamato E, Miyazaki J. Acinar-to-ductal metaplasia induced by adenovirus-mediated pancreatic expression of Isl1. PLoS ONE. 2012;7:e47536 pubmed publisher
O Neill P, Mak S, Fritzsch B, Ladher R, Baker C. The amniote paratympanic organ develops from a previously undiscovered sensory placode. Nat Commun. 2012;3:1041 pubmed publisher
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Huettl R, Haehl T, Huber A. Fasciculation and guidance of spinal motor axons in the absence of FGFR2 signaling. PLoS ONE. 2012;7:e41095 pubmed publisher
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Li H, Kloosterman W, Fekete D. MicroRNA-183 family members regulate sensorineural fates in the inner ear. J Neurosci. 2010;30:3254-63 pubmed publisher
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product information
Internal ID :
4236
Name :
39.4D5
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Depositor Website :
http://sklad.cumc.columbia.edu/jessell/
Date Deposited :
11/3/95
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Islet-1 & Islet-2 homeobox
Antigen Species :
Rat
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Chicken,Ferret,Fish,Frog,Human,Mouse,Rat,Zebrafish,altricial fish larva
Species Tested (legacy) :
all tested (rat, mouse, chicken, frog, zebra fish, human) are positive
Initial Publication Pubmed ID :
1350865 7528105
Depositor Notes (Special Instructions) :
MN, DRG & other nuclei characterized extensively by immunocytochemistry; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006) TEST
Collections :
Cardiac development,Human,Neurodevelopment,Transcription factors,Xenopus
Search Keywords :
Jessell, Thomas M / Brenner-Morton, Susan, Islet-1 Islet-2 homeobox, Rat, MIgG2b, Rat/Mouse/Chicken/Frog/Zebrafish/Ferret/Human, Isl1 & Isl2, ISL1, ISL LIM homeobox 1, ISL2; ISL LIM homeobox 2, AB_2314683, Monoclonal
Antigen Molecular Weight :
Predicted: 39kDa; Apparent: 39kDa
Gene :
Isl1 & Isl2
Alternate Gene Name(s) :
ISL1, ISL LIM homeobox 1, ISL2; ISL LIM homeobox 2
Uniprot ID :
P61374 P50480
Antibody Registry ID :
AB_2314683
Synonyms (Alternate Clone Names) :
anti-islet1; anti-isl1;anti-islet2; anti-isl2
Immunogen :
Recombinant fusion protein containing aa 178-349 of Isl1 protein (C-terminus)
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 178-349 of Isl1 protein
Epitope Map PubMed ID :
1350865
Recommended Applications :
Chromatin Immunoprecipitation,Gel Supershift,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
14638744 16899237 7528105 8929535 10491257 9890440 10864957 11336495 11596060 11877374 12121626 12357432 14656152 14638744 14993186 14973 11818560 [289 15464286 16260730 16672347 16899237 16815098 19571884 21334323 22318233 12150931 8124714 10704832 9507132 15614781 22833130 11301207 15525781 22275004 23077655 22554084 21689645 21471154 22003118 20203184 20146251 20080554 19924821 20549713 19379735 29848488
Immunofluorescence Pubmed IDs :
20549713 19379735 28817802 24760871 27100776 31313880 30311912
Western Blot Pubmed IDs :
21388963
Chromatin Immunoprecipitation Pubmed IDs :
24674670
FFPE Pubmed IDs :
29848488
Gel Supershift Pubmed IDs :
19502415
Pubmed IDs :
22275004 23077655 22554084 21689645 21471154 22003118 20203184 20146251 20080554 19924821
Additional Information :
also detects Islet-2. The protein region used for immunization lacks the LIM motifs shared with other proteins.
DSHB Growth Medium :
Iscove's
References (legacy) :
Science 256, 1555-1560.; Cell 73, 673-686.; Cell 87, 661-673.; Dev. Biol. 214, 60-71.; J. Neurosci. Res. 55, 119-126.; J. Neurosci. 20(13), 4992-5000.; Dev. Biol. 233, 271-290.; J. Comp. Neurol. 439, 368-383.; Genes Dev. 16, 548-553.; Curr. Biol. 12, 1157-1163.; J. Comp. Neurol. 453, 57-70.; Cell 110, 237-249.; Biotechnol. Prog. 19, 1756-1761.; Invest. Ophthalmol. Vis. Sci. 44(12), 5404-5409.; Development 131, 1319-1330.; Development 131, 1017-1028.; J. Neurosci. 24(44), 9962-9970.; Neuroscience 128, 785-796.; Dev. Dyn. 232, 313-324.; PNAS 102(45), 16421-16425.; Development 133, 2137-2147.; Dev. Biol. 298, 22-31.; Genes Dev. 21, 531-536.; Genes Dev. 22, 3320-3334.; Gene Expr. Patterns 7, 57-65.; Dev. Biol. 353, 10-18.; Hear. Res. 278, 86-95.; J. Biol. Chem. 287(24), 20483-20489.; Development 139, 1125-1132.; J. Biol. Chem. 287(29), 24255-24262.; J. Comp. Neurol. 520, 330-363.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US