product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Islet-1 specific homeobox
catalog :
39.3F7
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
39.3F7
reactivity :
mouse, rat, chicken, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, FACS, immunohistochemistry - paraffin section
citations: 16
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4c
Smith R, Huang Y, Tian T, Vojtasova D, Mesalles Naranjo O, Pollard S, et al. The Transcription Factor Foxg1 Promotes Optic Fissure Closure in the Mouse by Suppressing Wnt8b in the Nasal Optic Stalk. J Neurosci. 2017;37:7975-7993 pubmed publisher
  • immunohistochemistry; mouse; 1:500; fig s5
Mao C, Agca C, Mocko Strand J, Wang J, Ullrich Lüter E, Pan P, et al. Substituting mouse transcription factor Pou4f2 with a sea urchin orthologue restores retinal ganglion cell development. Proc Biol Sci. 2016;283:20152978 pubmed publisher
  • immunocytochemistry; mouse
Yang J, Kaur K, Ong L, Eisenberg C, Eisenberg L. Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors. Stem Cells Int. 2015;2015:270428 pubmed publisher
  • immunohistochemistry; mouse; 1:400
Chai G, Zhou L, Manto M, Helmbacher F, Clotman F, Goffinet A, et al. Celsr3 is required in motor neurons to steer their axons in the hindlimb. Nat Neurosci. 2014;17:1171-9 pubmed publisher
Schick E, McCaffery S, Keblish E, Thakurdin C, Emerson M. Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina. Sci Rep. 2019;9:9358 pubmed publisher
Ghimire S, Ratzan E, Deans M. A non-autonomous function of the core PCP protein VANGL2 directs peripheral axon turning in the developing cochlea. Development. 2018;145: pubmed publisher
Dvorakova M, Jahan I, Macova I, Chumak T, Bohuslavova R, Syka J, et al. Incomplete and delayed Sox2 deletion defines residual ear neurosensory development and maintenance. Sci Rep. 2016;6:38253 pubmed publisher
Ediger B, Du A, Liu J, Hunter C, Walp E, Schug J, et al. Islet-1 Is essential for pancreatic ?-cell function. Diabetes. 2014;63:4206-17 pubmed publisher
Hua Z, Smallwood P, Nathans J. Frizzled3 controls axonal development in distinct populations of cranial and spinal motor neurons. elife. 2013;2:e01482 pubmed publisher
Audouard E, Schakman O, Rene F, Huettl R, Huber A, Loeffler J, et al. The Onecut transcription factor HNF-6 regulates in motor neurons the formation of the neuromuscular junctions. PLoS ONE. 2012;7:e50509 pubmed publisher
Roy A, Francius C, Rousso D, Seuntjens E, Debruyn J, Luxenhofer G, et al. Onecut transcription factors act upstream of Isl1 to regulate spinal motoneuron diversification. Development. 2012;139:3109-19 pubmed publisher
Gutierrez C, McNally M, Canto Soler M. Cytoskeleton proteins previously considered exclusive to ganglion cells are transiently expressed by all retinal neuronal precursors. BMC Dev Biol. 2011;11:46 pubmed publisher
Du A, Hunter C, Murray J, Noble D, Cai C, Evans S, et al. Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas. Diabetes. 2009;58:2059-69 pubmed publisher
Fischer A, Foster S, Scott M, Sherwood P. Transient expression of LIM-domain transcription factors is coincident with delayed maturation of photoreceptors in the chicken retina. J Comp Neurol. 2008;506:584-603 pubmed
Sanders E, Walter M, Parker E, Aramburo C, Harvey S. Opticin binds retinal growth hormone in the embryonic vitreous. Invest Ophthalmol Vis Sci. 2003;44:5404-9 pubmed
Thor S, Ericson J, Brannstrom T, Edlund T. The homeodomain LIM protein Isl-1 is expressed in subsets of neurons and endocrine cells in the adult rat. Neuron. 1991;7:881-9 pubmed
product information
Internal ID :
4230
Name :
39.3F7
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Depositor Website :
http://sklad.cumc.columbia.edu/jessell/
Date Deposited :
3/7/07
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Islet-1 specific homeobox
Antigen Species :
rat
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chicken,Fish,Frog,Mouse,Rat,Zebrafish
Species Tested (legacy) :
all tested (rat, mouse, chick, frog, zebra fish) are positive
Initial Publication Pubmed ID :
14638744
Depositor Notes (Special Instructions) :
Islet-1 specific ; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006)
Collections :
Human,Neurodevelopment,Transcription factors
Search Keywords :
Jessell, Thomas M / Brenner-Morton, Susan, Islet-1 specific homeobox, Rat, MIgG1, Rat/Mouse/Chicken/Frog/Zebrafish, Isl1, ISL1, ISL LIM homeobox 1, AB_1157901, Monoclonal
Antigen Molecular Weight :
Predicted: 39kDa; Apparent: 39kDa
Gene :
Isl1
Alternate Gene Name(s) :
ISL1, ISL LIM homeobox 1
Uniprot ID :
P61374
Antibody Registry ID :
AB_1157901
Synonyms (Alternate Clone Names) :
anti-islet1; anti-isl1
Immunogen :
Islet-I (rat) C-terminus
Immunogen Sequence :
amino acid residues 178- 349
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
No
Recommended Applications :
FACS,Gel Supershift,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
22833130 21781303 18072193 23227180 30087351 31361752 31376504
FACS Pubmed IDs :
30087351
Gel Supershift Pubmed IDs :
19502415
Pubmed IDs :
1764243
Additional Information :
The antibody pufiication procedure and the descrition of the plasmid is described in 1764243
DSHB Growth Medium :
Iscove's
References (legacy) :
Invest. Ophthalmol. Vis. Sci. 44(12), 5404-5409.; Dev. Biol. 298, 22-31.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US