product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Ryanodine receptor
catalog :
34C
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
34C
reactivity :
human, mouse, rat, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 19
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; rat; loading ...; fig 1c
  • immunohistochemistry; rat; loading ...; fig 2a
  • immunocytochemistry; mouse; loading ...; fig 11a
Vierra N, Kirmiz M, van der List D, Santana L, Trimmer J. Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in mammalian neurons. elife. 2019;8: pubmed publisher
  • immunohistochemistry; zebrafish ; 1:500; loading ...; fig 1a
Linsley J, Hsu I, Groom L, Yarotskyy V, Lavorato M, Horstick E, et al. Congenital myopathy results from misregulation of a muscle Ca2+ channel by mutant Stac3. Proc Natl Acad Sci U S A. 2017;114:E228-E236 pubmed publisher
  • western blot; mouse; 1:100; loading ...; fig 6
Horvath D, Murphy R, Mollica J, Hayes A, Goodman C. The effect of taurine and β-alanine supplementation on taurine transporter protein and fatigue resistance in skeletal muscle from mdx mice. Amino Acids. 2016;48:2635-2645 pubmed
  • western blot; mouse; fig 13
Cong X, Doering J, Mázala D, Chin E, Grange R, Jiang H. The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle. Skelet Muscle. 2016;6:17 pubmed publisher
Keil K, Sethi S, Lein P. Sex-Dependent Effects of 2,2',3,5',6-Pentachlorobiphenyl on Dendritic Arborization of Primary Mouse Neurons. Toxicol Sci. 2019;168:95-109 pubmed publisher
Kesireddy V. Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury. Int J Nanomedicine. 2016;11:1461-73 pubmed publisher
Rebbeck R, Willemse H, Groom L, Casarotto M, Board P, Beard N, et al. Regions of ryanodine receptors that influence activation by the dihydropyridine receptor β1a subunit. Skelet Muscle. 2015;5:23 pubmed publisher
Murayama T, Kurebayashi N, Yamazawa T, Oyamada H, Suzuki J, Kanemaru K, et al. Divergent Activity Profiles of Type 1 Ryanodine Receptor Channels Carrying Malignant Hyperthermia and Central Core Disease Mutations in the Amino-Terminal Region. PLoS ONE. 2015;10:e0130606 pubmed publisher
Paolini C, Quarta M, Wei LaPierre L, Michelucci A, Nori A, Reggiani C, et al. Oxidative stress, mitochondrial damage, and cores in muscle from calsequestrin-1 knockout mice. Skelet Muscle. 2015;5:10 pubmed publisher
Li L, Mirza S, Richardson S, Gallant E, Thekkedam C, Pace S, et al. A new cytoplasmic interaction between junctin and ryanodine receptor Ca2+ release channels. J Cell Sci. 2015;128:951-63 pubmed publisher
Fero K, Bergeron S, Horstick E, Codore H, Li G, Ono F, et al. Impaired embryonic motility in dusp27 mutants reveals a developmental defect in myofibril structure. Dis Model Mech. 2014;7:289-98 pubmed publisher
Corona B, Machingal M, Criswell T, Vadhavkar M, Dannahower A, Bergman C, et al. Further development of a tissue engineered muscle repair construct in vitro for enhanced functional recovery following implantation in vivo in a murine model of volumetric muscle loss injury. Tissue Eng Part A. 2012;18:1213-28 pubmed publisher
Murphy R. Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1. J Appl Physiol (1985). 2011;110:820-5 pubmed publisher
Murphy R, Lamb G. Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch. J Biol Chem. 2009;284:7811-9 pubmed publisher
Goonasekera S, Beard N, Groom L, Kimura T, Lyfenko A, Rosenfeld A, et al. Triadin binding to the C-terminal luminal loop of the ryanodine receptor is important for skeletal muscle excitation contraction coupling. J Gen Physiol. 2007;130:365-78 pubmed
Beutner G, Sharma V, Giovannucci D, Yule D, Sheu S. Identification of a ryanodine receptor in rat heart mitochondria. J Biol Chem. 2001;276:21482-8 pubmed
Kirkeby S, Høyer P. Binding properties of the galactose-detecting lectin Pseudomonas aeruginosa agglutinin (PA-IL) to skeletal muscle fibres. Quantitative precipitation and precipitation inhibition assays. Histochem J. 1999;31:485-93 pubmed
Tong J, Oyamada H, Demaurex N, Grinstein S, McCarthy T, MacLennan D. Caffeine and halothane sensitivity of intracellular Ca2+ release is altered by 15 calcium release channel (ryanodine receptor) mutations associated with malignant hyperthermia and/or central core disease. J Biol Chem. 1997;272:26332-9 pubmed
Airey J, Beck C, Murakami K, Tanksley S, Deerinck T, Ellisman M, et al. Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle. J Biol Chem. 1990;265:14187-94 pubmed
product information
Internal ID :
4255
Name :
34C
Depositor Name :
Airey, J. / Sutko, J.
Depositor Institution :
University of Nevada
Date Deposited :
2/29/96
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Ryanodine receptor
Antigen Species :
chicken
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Amphibian,Avian,Fish,Mammal
Species Tested (legacy) :
mammalian, avian, amphibian, piscine-widely cross-reactive
Initial Publication Pubmed ID :
2387846
Depositor Notes (Special Instructions) :
This antigen is a calcium release channel localized in the sarcoplasmic reticulum.
Collections :
Cell signaling,Human,Neurodevelopment,Skeletal muscle
Search Keywords :
Judith Airey & John Sutko, Ryanodine receptor, Chicken , MIgG1, Mammal/avian/amphibian/fish, RYR1/RYR2, CCO, MHS, MHS1, PPP1R137, RYR, AB_528457, Monoclonal, Human, Immunohistochemistry/Immunofluorescence/Western blot/Immunoprecipitation
Antigen Molecular Weight :
562.7 kDa
Gene :
RYR1/RYR2
Alternate Gene Name(s) :
CCO, MHS, MHS1, PPP1R137, RYR
Uniprot ID :
P21817
Antibody Registry ID :
AB_528457
Immunogen :
Microsomal membrane preparation from skeletal muscle
Immunogen Sequence :
Purified alpha and beta ryanodine receptor
Clonality :
Monoclonal
Myeloma Strain :
P3x63Ag8.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
a.a. 2756-2803 (RyR1) and a.a. 2722-2769 (RyR2) )
Epitope Map PubMed ID :
9334205 17606610 2387846 21262961
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
27114706 26075051 22439962 2387846
Immunofluorescence Pubmed IDs :
2387846 10475576 11297554 19144634 26203350 17846166 30395321
Western Blot Pubmed IDs :
2387846 21088205 10475576 11297554 19144634 26115329 25609705
Immunoprecipitation Pubmed IDs :
2387846 10475576 25609705
FFPE Pubmed IDs :
22439962
Additional Information :
RRID:AB_528457
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Biol. Chem. 265, 14187-14194.; Histochem. J. 31, 485-493.; J. Biol. Chem. 276(24), 21482-21488.; J. Biol. Chem. 284(12), 7811-7819.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US