fasciclin II (Drosophila, all isoforms) | Developmental Studies Hybridoma Bank 34B3 anti-Fasciclin II product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

fasciclin II (Drosophila, all isoforms)

catalog :

34B3 anti-Fasciclin II

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

34B3 anti-Fasciclin II

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Wang M, Chen P, Wang C, Lai T, Tsai P, Cheng Y, et al. Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response. PLoS Genet. 2016;12:e1006362 pubmed publisher
Halberg K, Rainey S, Veland I, Neuert H, Dornan A, Klämbt C, et al. The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules. Nat Commun. 2016;7:11266 pubmed publisher
Wang J, Beck E, McCabe B. A modular toolset for recombination transgenesis and neurogenetic analysis of Drosophila. PLoS ONE. 2012;7:e42102 pubmed publisher
Beck E, Gasque G, Imlach W, Jiao W, Jiwon Choi B, Wu P, et al. Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag. J Neurosci. 2012;32:7058-73 pubmed publisher
Nicolaï M, Lasbleiz C, Dura J. Gain-of-function screen identifies a role of the Src64 oncogene in Drosophila mushroom body development. J Neurobiol. 2003;57:291-302 pubmed
Grenningloh G, Rehm E, Goodman C. Genetic analysis of growth cone guidance in Drosophila: fasciclin II functions as a neuronal recognition molecule. Cell. 1991;67:45-57 pubmed

product information

Internal ID :

3544

Name :

34B3 anti-Fasciclin II

Depositor Name :

Goodman, C.

Depositor Institution :

University of California, Berkeley

Date Deposited :

11/1/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

fasciclin II (Drosophila, all isoforms)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

1913818

Depositor Notes (Special Instructions) :

Extracellular cell binding.

Collections :

Cell adhesion,Cell markers,Drosophila antigens,Neurodevelopment

Search Keywords :

Goodman, C., fasciclin II (Drosophila, all isoforms), Drosophila, MIgG1, kappa light chain, Drosophila, Fas2, FasII; CG3665, AB_528236, Monoclonal, Drosophila antigens/Cell markers/Neurodevelopment/Cell adhesion, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: three main isoforms of 97, 90, and 86 kDa

Gene :

Fas2

Alternate Gene Name(s) :

FasII; CG3665

Uniprot ID :

P34082

Antibody Registry ID :

AB_528236

Immunogen :

recombinant fusion protein, trpE fused to two different, nonoverlapping fragments of Drosophila fasII cDNA: a 1227 bp XhoI-HindIII fragment encopassing Ig domains 2-5 and a 496 bp SalI-ClaI fragment including fibronectin domain 2.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63.Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

extracellular domain of all four isoforms

Epitope Map PubMed ID :

22593074

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

1913818

Immunofluorescence Pubmed IDs :

22593074 22848718 14608664 27072072 27736876

Western Blot Pubmed IDs :

22593074 27072072

Additional Information :

The initial publication and the immunogen information are only putative and may or may not be correct. The depositor didn't specify any initial publication and didn't indicate the immunogen used. The mAb described in [PMID 1913818] has the generic name: anti-fasciclin II Mab.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Neurosci. 32(20), 7058-7073.

more info or order :

Developmental Studies Hybridoma Bank product webpage