Contactin-2, neuronal marker | Developmental Studies Hybridoma Bank 3.1C12 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Contactin-2, neuronal marker

catalog :

3.1C12

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3.1C12

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; fig s4i	Amin N, Bai G, Klug J, Bonanomi D, Pankratz M, Gifford W, et al. Loss of motoneuron-specific microRNA-218 causes systemic neuromuscular failure. Science. 2015;350:1525-9 pubmed publisher
	Kastriti M, Sargiannidou I, Kleopa K, Karagogeos D. Differential modulation of the juxtaparanodal complex in Multiple Sclerosis. Mol Cell Neurosci. 2015;67:93-103 pubmed publisher
	Favero C, Henshaw R, Grimsley Myers C, Shrestha A, Beier D, Dwyer N. Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain. J Comp Neurol. 2013;521:677-96 pubmed publisher
	Savvaki M, Theodorakis K, Zoupi L, Stamatakis A, Tivodar S, Kyriacou K, et al. The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS. J Neurosci. 2010;30:13943-54 pubmed publisher
	Ma Q, Futagawa T, Yang W, Jiang X, Zeng L, Takeda Y, et al. A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis. Nat Cell Biol. 2008;10:283-94 pubmed publisher
	Soares S, Traka M, von Boxberg Y, Bouquet C, Karagogeos D, Nothias F. Neuronal and glial expression of the adhesion molecule TAG-1 is regulated after peripheral nerve lesion or central neurodegeneration of adult nervous system. Eur J Neurosci. 2005;21:1169-80 pubmed
	Rickman D, Tyagi R, Zhu X, Bobek M, Song S, Blaivas M, et al. The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas. Cancer Res. 2001;61:2162-8 pubmed
	Karagogeos D, Pourquié C, Kyriakopoulou K, Tavian M, Stallcup W, Peault B, et al. Expression of the cell adhesion proteins BEN/SC1/DM-GRASP and TAG-1 defines early steps of axonogenesis in the human spinal cord. J Comp Neurol. 1997;379:415-27 pubmed
	Dodd J, Morton S, Karagogeos D, Yamamoto M, Jessell T. Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons. Neuron. 1988;1:105-16 pubmed
	Chen E, Chiu A. Early stages in the development of spinal motor neurons. J Comp Neurol. 1992;320:291-303 pubmed

product information

Internal ID :

2593

Name :

3.1C12

Depositor Name :

Jessell, T.M. / Dodd, J. / Brenner-Morton, S.

Depositor Institution :

Center for Neurobiology & Behavior, Columbia University

Date Deposited :

8/15/92

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Contactin-2, neuronal marker

Antigen Species :

rat

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Rat

Species Tested (legacy) :

rat, some mouse strains, human

Initial Publication Pubmed ID :

3272160

Depositor Notes (Special Instructions) :

Stains motoneurons, commissural neurons and central afferents of the DRG. Works on western blots of purified TAG-1.

Collections :

Cell adhesion,Cell signaling,Human,Neurodevelopment

Search Keywords :

Jessell, T.M. / Dodd, J. / Brenner-Morton, S., Contactin-2, neuronal marker, rat, MIgG1, rat/human, Cntn2, Tax1, Tag1, AB_2229763, monoclonal, Cell adhesion/Cell signaling/Human/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/FFPE, tag-1

Antigen Molecular Weight :

Predicted: 104 kDa; Apparent: 120 kDa (nonreducing conditions), 135 kDa (reducing conditions)

Gene :

Cntn2

Alternate Gene Name(s) :

Tax1, Tag1

Uniprot ID :

P22063

Antibody Registry ID :

AB_2229763

Synonyms (Alternate Clone Names) :

1C12

Immunogen :

Rat (E14-E15) spinal cord membranes

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Recommended Applications :

FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes-purified protein

Immunohistochemistry Pubmed IDs :

9067833 11280781 15813926

Immunofluorescence Pubmed IDs :

3272160 26070930 15813926 1535349 20962216 31390561

Western Blot Pubmed IDs :

3272160

Immunoprecipitation Pubmed IDs :

3272160 22821687 18278038 20962216

FFPE Pubmed IDs :

11280781

Additional Information :

This antibody may work with some mouse strains.

DSHB Growth Medium :

Iscove's

References (legacy) :

Neuron 1, 105-116.; J. Comp. Neurol. 379, 415-427.; Cancer Res. 61, 2162-2168.

more info or order :

Developmental Studies Hybridoma Bank product webpage