product summary
company name : 
Developmental Studies Hybridoma Bank
product type : 
antibody
product name : 
neurofilament (NF-M)
catalog : 
2H3
clonality : 
monoclonal
host : 
mouse
conjugate : 
nonconjugated
clone name : 
2H3
reactivity : 
human, mouse, rat
application : 
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order : 
citations: 131
| Published Application/Species/Sample/Dilution | Reference | 
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  | Espinosa Medina I, Saha O, Boismoreau F, Chettouh Z, Rossi F, Richardson W, et al. The sacral autonomic outflow is sympathetic. Science. 2016;354:893-897 pubmed  
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Hostikka S, Gong J, Carpenter E. Axial and appendicular skeletal transformations, ligament alterations, and motor neuron loss in Hoxc10 mutants. Int J Biol Sci. 2009;5:397-410 pubmed  
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Tannemaat M, Korecka J, Ehlert E, Mason M, van Duinen S, Boer G, et al. Human neuroma contains increased levels of semaphorin 3A, which surrounds nerve fibers and reduces neurite extension in vitro. J Neurosci. 2007;27:14260-4 pubmed  
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Schmidt H, Stonkute A, Jüttner R, Schaffer S, Buttgereit J, Feil R, et al. The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord. J Cell Biol. 2007;179:331-40 pubmed  
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Shibasaki K, Suzuki M, Mizuno A, Tominaga M. Effects of body temperature on neural activity in the hippocampus: regulation of resting membrane potentials by transient receptor potential vanilloid 4. J Neurosci. 2007;27:1566-75 pubmed  
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Anderson R, Stottmann R, Choi M, Klingensmith J. Endogenous bone morphogenetic protein antagonists regulate mammalian neural crest generation and survival. Dev Dyn. 2006;235:2507-20 pubmed  
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Diks S, Bink R, van de Water S, Joore J, van Rooijen C, Verbeek F, et al. The novel gene asb11: a regulator of the size of the neural progenitor compartment. J Cell Biol. 2006;174:581-92 pubmed  
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Jevince A, Kadison S, Pittman A, Chien C, Kaprielian Z. Distribution of EphB receptors and ephrin-B1 in the developing vertebrate spinal cord. J Comp Neurol. 2006;497:734-50 pubmed  
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Uemura M, Takeichi M. Alpha N-catenin deficiency causes defects in axon migration and nuclear organization in restricted regions of the mouse brain. Dev Dyn. 2006;235:2559-66 pubmed  
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Alpantaki K, McLaughlin D, Karagogeos D, Hadjipavlou A, Kontakis G. Sympathetic and sensory neural elements in the tendon of the long head of the biceps. J Bone Joint Surg Am. 2005;87:1580-3 pubmed  
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Hester M, Thompson J, Mills J, Liu Y, El Hodiri H, Weinstein M. Smad1 and Smad8 function similarly in mammalian central nervous system development. Mol Cell Biol. 2005;25:4683-92 pubmed  
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Yaron A, Huang P, Cheng H, Tessier Lavigne M. Differential requirement for Plexin-A3 and -A4 in mediating responses of sensory and sympathetic neurons to distinct class 3 Semaphorins. Neuron. 2005;45:513-23 pubmed  
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Bampton E, Taylor J. Effects of Schwann cell secreted factors on PC12 cell neuritogenesis and survival. J Neurobiol. 2005;63:29-48 pubmed  
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Yang D, Bierman J, Tarumi Y, Zhong Y, Rangwala R, Proctor T, et al. Coordinate control of axon defasciculation and myelination by laminin-2 and -8. J Cell Biol. 2005;168:655-66 pubmed  
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Ota M, Loebel D, O Rourke M, Wong N, Tsoi B, Tam P. Twist is required for patterning the cranial nerves and maintaining the viability of mesodermal cells. Dev Dyn. 2004;230:216-28 pubmed  
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Raft S, Nowotschin S, Liao J, Morrow B. Suppression of neural fate and control of inner ear morphogenesis by Tbx1. Development. 2004;131:1801-12 pubmed  
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Davy A, Aubin J, Soriano P. Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev. 2004;18:572-83 pubmed  
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Williams S, Mann F, Erskine L, Sakurai T, Wei S, Rossi D, et al. Ephrin-B2 and EphB1 mediate retinal axon divergence at the optic chiasm. Neuron. 2003;39:919-35 pubmed  
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Seibt J, Schuurmans C, Gradwhol G, Dehay C, Vanderhaeghen P, Guillemot F, et al. Neurogenin2 specifies the connectivity of thalamic neurons by controlling axon responsiveness to intermediate target cues. Neuron. 2003;39:439-52 pubmed  
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Mechta Grigoriou F, Giudicelli F, Pujades C, Charnay P, Yaniv M. c-jun regulation and function in the developing hindbrain. Dev Biol. 2003;258:419-31 pubmed  
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Chi C, Martinez S, Wurst W, Martin G. The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development. 2003;130:2633-44 pubmed  
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Mbiene J, Roberts J. Distribution of keratin 8-containing cell clusters in mouse embryonic tongue: evidence for a prepattern for taste bud development. J Comp Neurol. 2003;457:111-22 pubmed  
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Goldstein R, Drukker M, Reubinoff B, Benvenisty N. Integration and differentiation of human embryonic stem cells transplanted to the chick embryo. Dev Dyn. 2002;225:80-6 pubmed  
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Merlo G, Paleari L, Mantero S, Zerega B, Adamska M, Rinkwitz S, et al. The Dlx5 homeobox gene is essential for vestibular morphogenesis in the mouse embryo through a BMP4-mediated pathway. Dev Biol. 2002;248:157-69 pubmed  
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Takebayashi H, Nabeshima Y, Yoshida S, Chisaka O, Ikenaka K, Nabeshima Y. The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages. Curr Biol. 2002;12:1157-63 pubmed  
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Lonze B, Riccio A, Cohen S, Ginty D. Apoptosis, axonal growth defects, and degeneration of peripheral neurons in mice lacking CREB. Neuron. 2002;34:371-85 pubmed  
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Dunwoodie S, Clements M, Sparrow D, Sa X, Conlon R, Beddington R. Axial skeletal defects caused by mutation in the spondylocostal dysplasia/pudgy gene Dll3 are associated with disruption of the segmentation clock within the presomitic mesoderm. Development. 2002;129:1795-806 pubmed  
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Davies D, Holley M. Differential expression of alpha 3 and alpha 6 integrins in the developing mouse inner ear. J Comp Neurol. 2002;445:122-32 pubmed  
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Kawasaki T, Bekku Y, Suto F, Kitsukawa T, Taniguchi M, Nagatsu I, et al. Requirement of neuropilin 1-mediated Sema3A signals in patterning of the sympathetic nervous system. Development. 2002;129:671-80 pubmed  
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Watari N, Kameda Y, Takeichi M, Chisaka O. Hoxa3 regulates integration of glossopharyngeal nerve precursor cells. Dev Biol. 2001;240:15-31 pubmed  
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Gerhart J, Bast B, Neely C, Iem S, Amegbe P, Niewenhuis R, et al. MyoD-positive myoblasts are present in mature fetal organs lacking skeletal muscle. J Cell Biol. 2001;155:381-92 pubmed  
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Schubert W, Kaprielian Z. Identification and characterization of a cell surface marker for embryonic rat spinal accessory motor neurons. J Comp Neurol. 2001;439:368-83 pubmed  
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Teng J, Takei Y, Harada A, Nakata T, Chen J, Hirokawa N. Synergistic effects of MAP2 and MAP1B knockout in neuronal migration, dendritic outgrowth, and microtubule organization. J Cell Biol. 2001;155:65-76 pubmed  
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Helmbacher F, Schneider Maunoury S, Topilko P, Tiret L, Charnay P. Targeting of the EphA4 tyrosine kinase receptor affects dorsal/ventral pathfinding of limb motor axons. Development. 2000;127:3313-24 pubmed  
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Medina Martinez O, Bradley A, Ramirez Solis R. A large targeted deletion of Hoxb1-Hoxb9 produces a series of single-segment anterior homeotic transformations. Dev Biol. 2000;222:71-83 pubmed  
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Leitges M, Neidhardt L, Haenig B, Herrmann B, Kispert A. The paired homeobox gene Uncx4.1 specifies pedicles, transverse processes and proximal ribs of the vertebral column. Development. 2000;127:2259-67 pubmed  
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Caton A, Hacker A, Naeem A, Livet J, Maina F, Bladt F, et al. The branchial arches and HGF are growth-promoting and chemoattractant for cranial motor axons. Development. 2000;127:1751-66 pubmed  
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Alvarez Bolado G, Zhou X, Voss A, Thomas T, Gruss P. Winged helix transcription factor Foxb1 is essential for access of mammillothalamic axons to the thalamus. Development. 2000;127:1029-38 pubmed  
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de la Cruz C, Der Avakian A, Spyropoulos D, Tieu D, Carpenter E. Targeted disruption of Hoxd9 and Hoxd10 alters locomotor behavior, vertebral identity, and peripheral nervous system development. Dev Biol. 1999;216:595-610 pubmed  
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Hildebrand J, Soriano P. Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice. Cell. 1999;99:485-97 pubmed  
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Dupé V, Ghyselinck N, Wendling O, Chambon P, Mark M. Key roles of retinoic acid receptors alpha and beta in the patterning of the caudal hindbrain, pharyngeal arches and otocyst in the mouse. Development. 1999;126:5051-9 pubmed  
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Rossel M, Capecchi M. Mice mutant for both Hoxa1 and Hoxb1 show extensive remodeling of the hindbrain and defects in craniofacial development. Development. 1999;126:5027-40 pubmed  
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Depew M, Liu J, Long J, Presley R, Meneses J, Pedersen R, et al. Dlx5 regulates regional development of the branchial arches and sensory capsules. Development. 1999;126:3831-46 pubmed  
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Nardelli J, Thiesson D, Fujiwara Y, Tsai F, Orkin S. Expression and genetic interaction of transcription factors GATA-2 and GATA-3 during development of the mouse central nervous system. Dev Biol. 1999;210:305-21 pubmed  
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Helmbacher F, Pujades C, Desmarquet C, Frain M, Rijli F, Chambon P, et al. Hoxa1 and Krox-20 synergize to control the development of rhombomere 3. Development. 1998;125:4739-48 pubmed  
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Xu W, Baribault H, Adamson E. Vinculin knockout results in heart and brain defects during embryonic development. Development. 1998;125:327-37 pubmed  
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Gavalas A, Studer M, Lumsden A, Rijli F, Krumlauf R, Chambon P. Hoxa1 and Hoxb1 synergize in patterning the hindbrain, cranial nerves and second pharyngeal arch. Development. 1998;125:1123-36 pubmed  
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Manley N, Capecchi M. Hox group 3 paralogous genes act synergistically in the formation of somitic and neural crest-derived structures. Dev Biol. 1997;192:274-88 pubmed  
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Carpenter E, Goddard J, Davis A, Nguyen T, Capecchi M. Targeted disruption of Hoxd-10 affects mouse hindlimb development. Development. 1997;124:4505-14 pubmed  
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Gavalas A, Davenne M, Lumsden A, Chambon P, Rijli F. Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning. Development. 1997;124:3693-702 pubmed  
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Hoyt P, Bartholomew C, Davis A, Yutzey K, Gamer L, Potter S, et al. The Evi1 proto-oncogene is required at midgestation for neural, heart, and paraxial mesenchyme development. Mech Dev. 1997;65:55-70 pubmed  
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Yoshikawa Y, Fujimori T, McMahon A, Takada S. Evidence that absence of Wnt-3a signaling promotes neuralization instead of paraxial mesoderm development in the mouse. Dev Biol. 1997;183:234-42 pubmed  
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Inoue T, Chisaka O, Matsunami H, Takeichi M. Cadherin-6 expression transiently delineates specific rhombomeres, other neural tube subdivisions, and neural crest subpopulations in mouse embryos. Dev Biol. 1997;183:183-94 pubmed  
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Dupé V, Davenne M, Brocard J, Dolle P, Mark M, Dierich A, et al. In vivo functional analysis of the Hoxa-1 3' retinoic acid response element (3'RARE). Development. 1997;124:399-410 pubmed  
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Boulet A, Capecchi M. Targeted disruption of hoxc-4 causes esophageal defects and vertebral transformations. Dev Biol. 1996;177:232-49 pubmed  
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Luo J, Pasceri P, Conlon R, Rossant J, Giguere V. Mice lacking all isoforms of retinoic acid receptor beta develop normally and are susceptible to the teratogenic effects of retinoic acid. Mech Dev. 1995;53:61-71 pubmed  
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Bally Cuif L, Cholley B, Wassef M. Involvement of Wnt-1 in the formation of the mes/metencephalic boundary. Mech Dev. 1995;53:23-34 pubmed  
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Mansour S, Goddard J, Capecchi M. Mice homozygous for a targeted disruption of the proto-oncogene int-2 have developmental defects in the tail and inner ear. Development. 1993;117:13-28 pubmed  
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Zhang M, Kim H, Marshall H, Gendron Maguire M, Lucas D, Baron A, et al. Ectopic Hoxa-1 induces rhombomere transformation in mouse hindbrain. Development. 1994;120:2431-42 pubmed  
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McKay I, Muchamore I, Krumlauf R, Maden M, Lumsden A, Lewis J. The kreisler mouse: a hindbrain segmentation mutant that lacks two rhombomeres. Development. 1994;120:2199-211 pubmed  
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Wurst W, Auerbach A, Joyner A. Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum. Development. 1994;120:2065-75 pubmed  
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Kostic D, Capecchi M. Targeted disruptions of the murine Hoxa-4 and Hoxa-6 genes result in homeotic transformations of components of the vertebral column. Mech Dev. 1994;46:231-47 pubmed  
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Conlon R, Reaume A, Rossant J. Notch1 is required for the coordinate segmentation of somites. Development. 1995;121:1533-45 pubmed  
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Guthrie S, Pini A. Chemorepulsion of developing motor axons by the floor plate. Neuron. 1995;14:1117-30 pubmed  
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Dodd J, Morton S, Karagogeos D, Yamamoto M, Jessell T. Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons. Neuron. 1988;1:105-16 pubmed  
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Shah P, Briedis D, Robson H, Conterato J. In vitro activity of piperacillin compared with that of carbenicillin, ticarcillin, ampicillin, cephalothin, and cefamandole against Pseudomonas aeruginosa and Enterobacteriaceae. Antimicrob Agents Chemother. 1979;15:346-50 pubmed  
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product information
Internal ID : 
2575
Name : 
2H3
Depositor Name : 
Jessell, T.M. / Dodd, J.
Depositor Institution : 
HHMI/Columbia University
Depositor Website : 
http://sklad.cumc.columbia.edu/jessell/
Date Deposited : 
3/15/89
Allow Hybridoma Distribution : 
Yes
Cells Available (legacy) : 
Yes
Antigen : 
neurofilament (NF-M)
Antigen Species : 
Rat
Host Species : 
mouse
Isotype : 
MIgG1
Isotype for catalog (legacy) : 
IgG1
Positive Tested Species Reactivity : 
Human,Mouse,Rat
Species Tested (legacy) : 
rat, mouse
Initial Publication Pubmed ID : 
3272160
Collections : 
Cytoskeleton,Human,Neurodevelopment
Search Keywords : 
Jessell, Thomas M / Dodd, Jane, Neurofilament (165 kDa), Rat, MIgG1, Rat/Mouse, ,  Nef3, neurofilament-M, NF160, NF165, Nfm, NF-M , AB_2314897, Monoclonal
Antigen Molecular Weight : 
Apparent: 165 kDa
Gene : 
Nefm
Alternate Gene Name(s) : 
Nef3, neurofilament-M, NF160, NF165, Nfm, NF-M
Uniprot ID : 
P12839
Antibody Registry ID : 
AB_2314897
Synonyms (Alternate Clone Names) : 
anti-neurofilament; anti-NF-H antibody, nouse anti-NF anti-NF-medium-chain, anti-Nefm
Immunogen : 
Membrane preparations obtained from E14-E15.
Clonality : 
Monoclonal
Myeloma Strain : 
NS1
Epitope Mapped : 
No
Recommended Applications : 
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) : 
Yes
Immunohistochemistry Pubmed IDs : 
15995126 3272160 3272160 7541631 11596060 12121626 12203723 15702477 19439605 22513373 24453327 24190599 23562155 24347548 23769240 25007825 25253857 25017010 23801741 23136391 25033181 22833130     28887018      30082833
Immunofluorescence Pubmed IDs : 
27445146 18160633 23313848 23047679 12895419 17301165 22323722 10662642  28814342  25048219  25253857  26502195  29661920  27144312  27150099
Western Blot Pubmed IDs : 
15702477 16786562 22057101  25048219
Pubmed IDs : 
111609 16894609 8555108 8660891 9409668 10725250 12736208 7789282 11891657 15037550 10642795 10433912 11923214 9053316 10529422 19580868 9367425 9463359 9806922 10887087 15899870 10589677 19623272 9256345 9126293 11830568 7918106 10804169 11988169 8555112 9441667 8223243 12541313 12798298 7925021 10885747 12142028 10357893 15162501 15162501 15084464 20175189 10529420 17954614 18570257 16691566 11784044 12971893 7925010 9486805 15721238 9126297 7956823
DSHB Growth Medium : 
Iscove's
References (legacy) : 
Neuron 1, 105-116.; Neuron 14, 1117-1130.; J. Comp. Neurol. 439, 368-383.; Curr. Biol. 12, 1157-1163.; Dev. Dyn. 225, 80-86.; Neuron 39, 439-452.; J. Neurobiol. 63, 29-48.; J. Comp. Neurol. 497, 734-750.; J. Neurosci. 27(7), 1566-1575.; J. Neurosci.  29(19), 6276-6284.; Nat. Cell Biol. 13(12), 1415-1423.
more info or order : 
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Developmental Studies Hybridoma Bank
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