myosin heavy chain, fast, 2A (MYH2) | Developmental Studies Hybridoma Bank 2F7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

myosin heavy chain, fast, 2A (MYH2)

catalog :

2F7

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2F7

reactivity :

mouse, rat, cat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 2a immunohistochemistry; rat; loading ...; fig 2a	Sawano S, Komiya Y, Ichitsubo R, Ohkawa Y, Nakamura M, Tatsumi R, et al. A One-Step Immunostaining Method to Visualize Rodent Muscle Fiber Type within a Single Specimen. PLoS ONE. 2016;11:e0166080 pubmed publisher
	Rupert J, Schmidt E, Moreira Soto A, Herrera B, Vandeberg J, Butcher M. Myosin isoform expression in the prehensile tails of didelphid marsupials: functional differences between arboreal and terrestrial opossums. Anat Rec (Hoboken). 2014;297:1364-76 pubmed publisher
	Moncman C, Andrade M, McCool A, McMullen C, Andrade F. Development transitions of thin filament proteins in rat extraocular muscles. Exp Cell Res. 2013;319:23-31 pubmed publisher
	Bloemberg D, Quadrilatero J. Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis. PLoS ONE. 2012;7:e35273 pubmed publisher
	Bakkar N, Ladner K, Canan B, Liyanarachchi S, Bal N, Pant M, et al. IKK? and alternative NF-?B regulate PGC-1? to promote oxidative muscle metabolism. J Cell Biol. 2012;196:497-511 pubmed publisher
	Moncman C, Andrade F. Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles. Exp Cell Res. 2010;316:1958-65 pubmed publisher
	Hyatt J, Roy R, Rugg S, Talmadge R. Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles. J Exp Zool A Ecol Genet Physiol. 2010;313:45-57 pubmed publisher
	Zhong W, Lucas C, Hoh J. Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion. J Comp Physiol B. 2008;178:47-55 pubmed
	Toniolo L, Maccatrozzo L, Patruno M, Pavan E, Caliaro F, Rossi R, et al. Fiber types in canine muscles: myosin isoform expression and functional characterization. Am J Physiol Cell Physiol. 2007;292:C1915-26 pubmed
	Bergrin M, Bicer S, Lucas C, Reiser P. Three-dimensional compartmentalization of myosin heavy chain and myosin light chain isoforms in dog thyroarytenoid muscle. Am J Physiol Cell Physiol. 2006;290:C1446-58 pubmed
	Lucas C, Kang L, Hoh J. Monospecific antibodies against the three mammalian fast limb myosin heavy chains. Biochem Biophys Res Commun. 2000;272:303-8 pubmed

product information

Internal ID :

3303

Name :

2F7

Depositor Name :

Lucas, C.

Depositor Institution :

University of Sydney

Date Deposited :

9/26/07

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

myosin heavy chain, fast, 2A (MYH2)

Antigen Species :

cat

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Baboon,Bandicoot,Canine,Cat,Cheetah,Guinea Pig,Human,Marsupial,Mouse,Opossum,Rabbit,Rat,Tiger

Species Tested (legacy) :

guinea pig, rat, mouse, rabbit and cat

Initial Publication Pubmed ID :

10872844

Depositor Notes (Special Instructions) :

2F7 reacts specifically with 2A myosin heavy chain from rat limb muscle by western blot, and with 2A muscle fibres on guinea pig, rat, mouse, rabbit and cat limb muscles by immunohistochemistry.

Collections :

Cytoskeleton,Human

Search Keywords :

Lucas, C., myosin heavy chain, fast, 2A (MYH2), cat, MIgG1, kappa light chain, Guinea Pig/Rat/Mouse/Rabbit/Cat/Baboon/Opossum/Marsupial/Bandicoot/Human/Canine/Tiger/Cheetah, MYH2, MyHC-2A, AB_1161647, monoclonal, Cytoskeletal elements/Human, Immunohistochemistry/Western Blot/Immunofluorescence

Antigen Molecular Weight :

Predicted: 223 kDa

Gene :

MYH2

Alternate Gene Name(s) :

MyHC-2A

Uniprot ID :

M3WDH9

Antibody Registry ID :

AB_1161647

Immunogen :

Denatured masseter myosin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

10872844 24832677 17703312 22351927 17251320 19768738

Immunofluorescence Pubmed IDs :

23174654 22530000 20350540 31346037 31346037

Western Blot Pubmed IDs :

10872844 24832677 17703312 16371441

Additional Information :

On western blots of dog myosin, 2F7 reacts with all myosin isoforms.

DSHB Growth Medium :

Iscove's

References (legacy) :

Biochem. Biophys. Res. Commun. 272, 303-308.

more info or order :

Developmental Studies Hybridoma Bank product webpage