product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
FMR1 (residues #1-204)
catalog :
2F5-1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2F5-1
reactivity :
human, mouse, rat
application :
immunohistochemistry, immunocytochemistry, western blot knockout validation, immunohistochemistry knockout validation
citations: 19
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2a
  • western blot knockout validation; mouse; loading ...; fig 1f
Hodges J, Yu X, Gilmore A, Bennett H, Tjia M, Perna J, et al. Astrocytic Contributions to Synaptic and Learning Abnormalities in a Mouse Model of Fragile X Syndrome. Biol Psychiatry. 2017;82:139-149 pubmed publisher
Jain S, Welshhans K. Netrin-1 induces local translation of down syndrome cell adhesion molecule in axonal growth cones. Dev Neurobiol. 2016;76:799-816 pubmed publisher
Kyle A, Baker J, Gandolfo M, Reinsberg S, Minchinton A. Tissue penetration and activity of camptothecins in solid tumor xenografts. Mol Cancer Ther. 2014;13:2727-37 pubmed publisher
Gholizadeh S, Arsenault J, Xuan I, Pacey L, Hampson D. Reduced phenotypic severity following adeno-associated virus-mediated Fmr1 gene delivery in fragile X mice. Neuropsychopharmacology. 2014;39:3100-11 pubmed publisher
Mannari T, Sawa H, Furube E, Fukushima S, Nishikawa K, Nakashimna T, et al. Antidepressant-induced vascular dynamics in the hippocampus of adult mouse brain. Cell Tissue Res. 2014;358:43-55 pubmed publisher
Manousiouthakis E, Mendez M, Garner M, Exertier P, Makita T. Venous endothelin guides sympathetic innervation of the developing mouse heart. Nat Commun. 2014;5:3918 pubmed publisher
Furube E, Mannari T, Morita S, Nishikawa K, Yoshida A, Itoh M, et al. VEGF-dependent and PDGF-dependent dynamic neurovascular reconstruction in the neurohypophysis of adult mice. J Endocrinol. 2014;222:161-79 pubmed publisher
Morita S, Tatsumi K, Makinodan M, Okuda H, Kishimoto T, Wanaka A. Geissoschizine methyl ether, an alkaloid from the Uncaria hook, improves remyelination after cuprizone-induced demyelination in medial prefrontal cortex of adult mice. Neurochem Res. 2014;39:59-67 pubmed publisher
Swinehart I, Schlientz A, Quintanilla C, Mortlock D, Wellik D. Hox11 genes are required for regional patterning and integration of muscle, tendon and bone. Development. 2013;140:4574-82 pubmed publisher
Majima T, Takeuchi K, Sano K, Hirashima M, Zankov D, Tanaka Okamoto M, et al. An Adaptor Molecule Afadin Regulates Lymphangiogenesis by Modulating RhoA Activity in the Developing Mouse Embryo. PLoS ONE. 2013;8:e68134 pubmed publisher
Pacey L, Xuan I, Guan S, Sussman D, Henkelman R, Chen Y, et al. Delayed myelination in a mouse model of fragile X syndrome. Hum Mol Genet. 2013;22:3920-30 pubmed publisher
Morita S, Hourai A, Miyata S. Changes in pericytic expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs of adult mouse by osmotic stimulation. Cell Biochem Funct. 2014;32:51-61 pubmed publisher
Higashimori H, Morel L, Huth J, Lindemann L, Dulla C, Taylor A, et al. Astroglial FMRP-dependent translational down-regulation of mGluR5 underlies glutamate transporter GLT1 dysregulation in the fragile X mouse. Hum Mol Genet. 2013;22:2041-54 pubmed publisher
Ma S, Kwon H, Huang Z. A functional requirement for astroglia in promoting blood vessel development in the early postnatal brain. PLoS ONE. 2012;7:e48001 pubmed publisher
Gross C, Nakamoto M, Yao X, Chan C, Yim S, Ye K, et al. Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome. J Neurosci. 2010;30:10624-38 pubmed publisher
Zang J, Nosyreva E, Spencer C, Volk L, Musunuru K, Zhong R, et al. A mouse model of the human Fragile X syndrome I304N mutation. PLoS Genet. 2009;5:e1000758 pubmed publisher
Pacey L, Heximer S, Hampson D. Increased GABA(B) receptor-mediated signaling reduces the susceptibility of fragile X knockout mice to audiogenic seizures. Mol Pharmacol. 2009;76:18-24 pubmed publisher
Christie S, Akins M, Schwob J, Fallon J. The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits. J Neurosci. 2009;29:1514-24 pubmed publisher
Gabel L, Won S, Kawai H, McKinney M, Tartakoff A, Fallon J. Visual experience regulates transient expression and dendritic localization of fragile X mental retardation protein. J Neurosci. 2004;24:10579-83 pubmed
product information
Internal ID :
6750
Name :
2F5-1
Depositor Name :
Tartakoff, A.M. / Fallon, J.R.
Depositor Institution :
Case Western Reserve University
Depositor Website :
http://www.case.edu/med/pathology/faculty/tartakoff.html
Date Deposited :
9/10/10
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
FMR1 (residues #1-204)
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Human,Mouse,Rat
Species Tested (legacy) :
human, rat, mouse
Initial Publication Pubmed ID :
15564573
Depositor Notes (Special Instructions) :
Recommended starting concentration of 5-10 ug/ml for immunocytochemistry and immunohistochemistry. Staining of aldehyde fixed tissue requires antigen retrieval (Christie et al., 2009).
Collections :
Human
Search Keywords :
Tartakoff, Alan M. / Fallon, J.R., FMRP, Human, MIgG2b, Human/Rat/Mouse, FMR1, FMRP, FRAXA, POF, POF1, AB_10805421, Monoclonal
Antigen Molecular Weight :
Predicted: 71KDa
Gene :
FMR1
Alternate Gene Name(s) :
FMRP, FRAXA, POF, POF1
Uniprot ID :
Q06787
Antibody Registry ID :
AB_10805421
Immunogen :
N-terminal fragment of human FMRP-6X-His-tagged fusion protein.
Immunogen Sequence :
(residues #1-204)
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
Yes
Epitope Location or Sequence :
a.a. 1–204 (N-terminus)
Epitope Map PubMed ID :
15564573
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
15564573 19193898 23396537 23740941 24998620 24875861 25143448 24190599 23629811 24962546 24860149 24154528 23110156 23840823
Immunofluorescence Pubmed IDs :
26518186 31364704
Western Blot Pubmed IDs :
20011099 23396537 19351745 20702695
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Neurosci. 24, 10579-10583.; J. Neurosci. 29, 1514-1524.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US