product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
laminin, gamma-1
catalog :
2E8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2E8
reactivity :
human, rat, dog
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 23
Reference |
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Li J, Zhou L, Tran H, Chen Y, Nguyen N, Karasek M, et al. Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions. J Invest Dermatol. 2006;126:432-40 pubmed
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McMillan J, Akiyama M, Nakamura H, Shimizu H. Colocalization of multiple laminin isoforms predominantly beneath hemidesmosomes in the upper lamina densa of the epidermal basement membrane. J Histochem Cytochem. 2006;54:109-18 pubmed
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Schaart G, Hesselink R, Keizer H, Van Kranenburg G, Drost M, Hesselink M. A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections. Histochem Cell Biol. 2004;122:161-9 pubmed
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Murase S, Horwitz A. Deleted in colorectal carcinoma and differentially expressed integrins mediate the directional migration of neural precursors in the rostral migratory stream. J Neurosci. 2002;22:3568-79 pubmed
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Geberhiwot T, Wondimu Z, Salo S, Pikkarainen T, Kortesmaa J, Tryggvason K, et al. Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains. Matrix Biol. 2000;19:163-7 pubmed
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Sunderland W, Son Y, Miner J, Sanes J, Carlson S. The presynaptic calcium channel is part of a transmembrane complex linking a synaptic laminin (alpha4beta2gamma1) with non-erythroid spectrin. J Neurosci. 2000;20:1009-19 pubmed
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Church H, Aplin J. BeWo choriocarcinoma cells produce laminin 10. Biochem J. 1998;332 ( Pt 2):491-8 pubmed
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Peterson P, Pow C, Wilson D, Hendrickx A. Distribution of extracellular matrix components during early embryonic development in the macaque. Acta Anat (Basel). 1993;146:3-13 pubmed
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Peterson P, Pow C, Wilson D, Hendrickx A. Characterization of the extracellular matrix during somitogenesis in the long-tailed monkey (Macaca fascicularis). Acta Anat (Basel). 1993;146:223-33 pubmed
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Ljubimov A, Burgeson R, Butkowski R, Michael A, Sun T, Kenney M. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest. 1995;72:461-73 pubmed
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van der Ven P, Jap P, Ter Laak H, Nonaka I, Barth P, Sengers R, et al. Immunophenotyping of congenital myopathies: disorganization of sarcomeric, cytoskeletal and extracellular matrix proteins. J Neurol Sci. 1995;129:199-213 pubmed
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Dillner L, Dickerson K, Manthorpe M, Ruoslahti E, Engvall E. The neurite-promoting domain of human laminin promotes attachment and induces characteristic morphology in non-neuronal cells. Exp Cell Res. 1988;177:186-98 pubmed
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Gehlsen K, Dickerson K, Argraves W, Engvall E, Ruoslahti E. Subunit structure of a laminin-binding integrin and localization of its binding site on laminin. J Biol Chem. 1989;264:19034-8 pubmed
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Engvall E, Davis G, Dickerson K, Ruoslahti E, Varon S, Manthorpe M. Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site. J Cell Biol. 1986;103:2457-65 pubmed
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Sanes J, Engvall E, Butkowski R, Hunter D. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol. 1990;111:1685-99 pubmed
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Engvall E, Earwicker D, Haaparanta T, Ruoslahti E, Sanes J. Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. Cell Regul. 1990;1:731-40 pubmed
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product information
Internal ID :
2475
Name :
2E8
Depositor Name :
Engvall, E.S.
Depositor Institution :
La Jolla Cancer Research Foundation
Date Deposited :
4/8/87
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
laminin, gamma-1
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG2a, kappa light chain
Isotype for catalog (legacy) :
IgG2a, kappa light chain
Positive Tested Species Reactivity :
Canine,Guinea Pig,Human,Rat
Species Tested (legacy) :
human, rat
Initial Publication Pubmed ID :
2430984
Collections :
Cell adhesion,Extracellular matrix proteins,Human
Search Keywords :
Engvall, laminin, mouse, anti Laminin B2, Chain Anti-Laminin _1, MIgG2a, Canine,Guinea Pig,Human,Rat, AB_2134060, Monoclonal, LAMC1, MGC87297 LAMB2, ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Antigen Molecular Weight :
Predicted: 174 kDa; Apparent: 200 kDa
Gene :
LAMC1
Alternate Gene Name(s) :
MGC87297 LAMB2
Uniprot ID :
P11047
Antibody Registry ID :
AB_2134060
Synonyms (Alternate Clone Names) :
Anti-Laminin ?1,anti Laminin B2 Chain, LM?1
Immunogen :
human laminin from term placenta
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
X63.Ag8.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
Close to the junction between domains II and III
Epitope Map PubMed ID :
2211832 3164684 10842099
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes, reacts with one beta chain
Immunohistochemistry Pubmed IDs :
19325816 22415307 11978833 22203849 10648706 3164684 8434503 8317198 31343947
Immunofluorescence Pubmed IDs :
2430984 7723285 7608737 15322861 2211832 2099832 9601079 27047655 26864869 27167365 31303547
Western Blot Pubmed IDs :
10648706 2430984 2211832 2808408 2099832 16374451 10842099
Immunoprecipitation Pubmed IDs :
2430984 9601079 3164684 2099832 10842099
ELISA Pubmed IDs :
2430984 3164684 2099832 24681327 10842099
Depositor Growth Medium :
15% fetal bovine serum in RPMI 1640 with 1x pen/strep
Additional Information :
Better western blot results using non-reducing conditions. 2E8 doesn't cross-react with mouse antigen. 2E8 doesn't appear to affect laminin gamma-1 function.
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell Biol. 103, 2457-2465.; J. Tiss. Cult. Meth. 14, 31-36.; Lab. Invest. 72, 461-473.; Histochem. Cell Biol. 122, 161-169.; J. Histochem. Cytochem. 54(1), 109-118.; Int. J. Mol. Sci. 9, 1472-1488.
more info or order :
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