product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Dynein heavy chain
catalog :
2C11-2
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2C11-2
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; fruit fly; 1:100
Li L, Tian X, Zhu M, Bulgari D, Böhme M, Goettfert F, et al. Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons. J Neurosci. 2014;34:8474-87 pubmed publisher
  • immunohistochemistry; fruit fly; 1:250; fig 1c
Williams L, Ganguly S, Loiseau P, Ng B, Palacios I. The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline. Development. 2014;141:176-86 pubmed publisher
Zheng J, Gao M, Huynh N, Tindell S, Vo H, McDonald W, et al. In vivo mapping of a dynamic ribonucleoprotein granule interactome in early Drosophila embryos. FEBS Open Bio. 2016;6:1248-1256 pubmed publisher
Kowanda M, Bergalet J, Wieczorek M, Brouhard G, Lecuyer E, Lasko P. Loss of function of the Drosophila Ninein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development. Biol Open. 2016;5:1040-51 pubmed publisher
Melkov A, Simchoni Y, Alcalay Y, Abdu U. Dynamic microtubule organization and mitochondrial transport are regulated by distinct Kinesin-1 pathways. Biol Open. 2015;4:1696-706 pubmed publisher
Otani T, Oshima K, Kimpara A, Takeda M, Abdu U, Hayashi S. A transport and retention mechanism for the sustained distal localization of Spn-F-IKKε during Drosophila bristle elongation. Development. 2015;142:2338-51 pubmed publisher
Hayashi R, Wainwright S, Liddell S, Pinchin S, Horswell S, Ish Horowicz D. A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila. G3 (Bethesda). 2014;4:749-60 pubmed publisher
Bartsch T, Longoria R, Florin E, Shubeita G. Lipid droplets purified from Drosophila embryos as an endogenous handle for precise motor transport measurements. Biophys J. 2013;105:1182-91 pubmed publisher
Dix C, Soundararajan H, Dzhindzhev N, Begum F, Suter B, Ohkura H, et al. Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs. J Cell Biol. 2013;202:479-94 pubmed publisher
Liu Y, Salter H, Holding A, Johnson C, Stephens E, Lukavsky P, et al. Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein. Genes Dev. 2013;27:1233-46 pubmed publisher
Amrute Nayak M, Bullock S. Single-molecule assays reveal that RNA localization signals regulate dynein-dynactin copy number on individual transcript cargoes. Nat Cell Biol. 2012;14:416-23 pubmed publisher
Darnell J, Van Driesche S, Zhang C, Hung K, Mele A, Fraser C, et al. FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism. Cell. 2011;146:247-61 pubmed publisher
Ally S, Larson A, Barlan K, Rice S, Gelfand V. Opposite-polarity motors activate one another to trigger cargo transport in live cells. J Cell Biol. 2009;187:1071-82 pubmed publisher
Kim H, Ling S, Rogers G, Kural C, Selvin P, Rogers S, et al. Microtubule binding by dynactin is required for microtubule organization but not cargo transport. J Cell Biol. 2007;176:641-51 pubmed
Papoulas O, Hays T, Sisson J. The golgin Lava lamp mediates dynein-based Golgi movements during Drosophila cellularization. Nat Cell Biol. 2005;7:612-8 pubmed
Sharp D, Rogers G, Scholey J. Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos. Nat Cell Biol. 2000;2:922-30 pubmed
Sharp D, Brown H, Kwon M, Rogers G, Holland G, Scholey J. Functional coordination of three mitotic motors in Drosophila embryos. Mol Biol Cell. 2000;11:241-53 pubmed
product information
Internal ID :
6308
Name :
2C11-2
Depositor Name :
Scholey, J.M.
Depositor Institution :
University of California, Davis
Depositor Website :
https://www.mcb.ucdavis.edu/faculty-labs/scholey/
Date Deposited :
2/3/10
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Dynein heavy chain
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
10637305
Collections :
Drosophila antigens
Search Keywords :
Scholey, Jonathan M., Dynein heavy chain, Drosophila, MIgG1, Drosophila, Dhc, dynein heavy chain, Dhc64C, AB_2091523, Monoclonal
Gene :
Dhc
Alternate Gene Name(s) :
dynein heavy chain, Dhc64C
Uniprot ID :
P37276
Antibody Registry ID :
AB_2091523
Synonyms (Alternate Clone Names) :
anti-DHC mAb alpha DHC mouse anti-Dhc
Immunogen :
preparation of Drosophila microtubule-associated proteins
Clonality :
Monoclonal
Myeloma Strain :
P3X63Ag8.653
Epitope Mapped :
No
Recommended Applications :
Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
10637305 11146657 15908943 24257625 24531791 24948803
Immunofluorescence Pubmed IDs :
24010661
Western Blot Pubmed IDs :
10637305 11146657 20038680 17325206 22366687 15908943 23918939 23723415
Immunoprecipitation Pubmed IDs :
21784246
Function Blocking Pubmed IDs :
10637305 11146657
DSHB Growth Medium :
Iscove's
References (legacy) :
Mole. Biol. Cell 11(1), 241-253.; Nat. Cell Biol. 2(12), 922-930.; J. Cell Biol. 187(7), 1071-1082.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US