hexokinase (Type I isozyme) | Developmental Studies Hybridoma Bank 2B6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

hexokinase (Type I isozyme)

catalog :

2B6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2B6

reactivity :

rat

application :

ELISA

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 7

Reference
Hashimoto M, Wilson J. Membrane potential-dependent conformational changes in mitochondrially bound hexokinase of brain. Arch Biochem Biophys. 2000;384:163-73 pubmed
Finney K, Messer J, DeWitt D, Wilson J. Monoclonal antibodies against rat brain hexokinase. Effects on catalytic function and binding to the outer mitochondrial membrane. J Biol Chem. 1984;259:8232-7 pubmed
Ureta T, Smith A, Wilson J. Hexokinase A from mammalian brain: comparative peptide mapping and immunological studies with monoclonal antibodies. Arch Biochem Biophys. 1986;246:419-27 pubmed
Smith A, Wilson J. A modified ELISA that selectively detects monoclonal antibodies recognizing native antigen. J Immunol Methods. 1986;94:31-5 pubmed
Wilson J, Smith A. Monoclonal antibodies against rat brain hexokinase. Utilization in epitope mapping studies and establishment of structure-function relationships. J Biol Chem. 1985;260:12838-43 pubmed
Smith A, Wilson J. Disposition of mitochondrially bound hexokinase at the membrane surface, deduced from reactivity with monoclonal antibodies recognizing epitopes of defined location. Arch Biochem Biophys. 1991;287:359-66 pubmed
Smith A, Wilson J. Epitopic regions recognized by monoclonal antibodies against rat brain hexokinase: association with catalytic and regulatory function. Arch Biochem Biophys. 1992;292:165-78 pubmed

product information

Internal ID :

1132

Name :

2B6

Depositor Name :

Wilson, J.E.

Depositor Institution :

Michigan State University, Dept. of Biochemistry & Molecular Biology

Date Deposited :

12/10/02

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

hexokinase (Type I isozyme)

Antigen Species :

rat

Host Species :

mouse

Isotype :

MIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Rat

Species Tested (legacy) :

rat

Initial Publication Pubmed ID :

6736033

Collections :

Enzymes,Neurodevelopment

Search Keywords :

Wilson, hexokinase (Type I isozyme), hk1, monoclonal, rat, IgG2a, ELISA

Gene :

HK1

Uniprot ID :

P05708

Antibody Registry ID :

AB_528281

Immunogen :

Type I isozyme of rat hexokinase

Clonality :

Monoclonal

Myeloma Strain :

SP2/0-Ag14

Epitope Mapped :

Recommended Applications :

ELISA

Immunoblotting (legacy) :

Pubmed IDs :

2413034 3963828 3782816 1716867 1370131 11147827

Additional Information :

The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. The functional effects of this antibody inhibits rat Type I hexokinase activity to >95%, the effect being on V max with no significant effect of Kms for either glucose or ATP [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178. RRID:AB_528281

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Biol. Chem. 259, 8232-8237.; J. Biol. Chem. 260, 12838-12843.; Arch. Biochem. Biophys. 246, 419-427.; J. Immunol. Meth. 94, 31-35.; Arch. Biochem. Biophys. 287, 359-366.; Arch. Biochem. Biophys. 292, 165-178.; Arch. Biochem. Biophys. 384, 163-173.

more info or order :

Developmental Studies Hybridoma Bank product webpage