product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Cut homeobox
catalog :
2B10
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2B10
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation
citations: 73
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; 1:20; loading ...; fig S3
Hessinger C, Technau G, Rogulja Ortmann A. The Drosophila Hox gene Ultrabithorax acts in both muscles and motoneurons to orchestrate formation of specific neuromuscular connections. Development. 2017;144:139-150 pubmed publisher
  • immunohistochemistry; fruit fly; 1:50; fig 2c
Huang D, Li X, Sun L, Huang P, Ying H, Wang H, et al. Regulation of Hippo signalling by p38 signalling. J Mol Cell Biol. 2016;8:328-37 pubmed publisher
  • immunohistochemistry; fruit fly; 1:100; fig 4
Tognon E, Kobia F, Busi I, Fumagalli A, De Masi F, Vaccari T. Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster. Autophagy. 2016;12:499-514 pubmed publisher
  • immunohistochemistry; fruit fly; 1:10; fig 7
Ho D, Pallavi S, Artavanis Tsakonas S. The Notch-mediated hyperplasia circuitry in Drosophila reveals a Src-JNK signaling axis. elife. 2015;4:e05996 pubmed publisher
  • immunohistochemistry; fruit fly; 1:50; loading ...; fig 4a
Pflanz R, Voigt A, Yakulov T, Jäckle H. Drosophila gene tao-1 encodes proteins with and without a Ste20 kinase domain that affect cytoskeletal architecture and cell migration differently. Open Biol. 2015;5:140161 pubmed publisher
Zhu J, Ordway A, Weber L, Buddika K, Kumar J. Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye. Development. 2018;145: pubmed publisher
Chen J, Xu N, Wang C, Huang P, Huang H, Jin Z, et al. Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells. Nat Cell Biol. 2018;20:152-161 pubmed publisher
Morrison C, Chen H, Cook T, Brown S, Treisman J. Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye. PLoS Genet. 2018;14:e1007173 pubmed publisher
Muzzopappa M, Murcia L, Milan M. Feedback amplification loop drives malignant growth in epithelial tissues. Proc Natl Acad Sci U S A. 2017;114:E7291-E7300 pubmed publisher
Ewen Campen B, Yang Zhou D, Fernandes V, González D, Liu L, Tao R, et al. Optimized strategy for in vivo Cas9-activation in Drosophila. Proc Natl Acad Sci U S A. 2017;114:9409-9414 pubmed publisher
Zhou Q, Neal S, Pignoni F. Mutant analysis by rescue gene excision: New tools for mosaic studies in Drosophila. Genesis. 2016;54:589-592 pubmed publisher
Tsao C, Ku H, Lee Y, Huang Y, Sun Y. Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs. PLoS ONE. 2016;11:e0163744 pubmed publisher
Nakazawa M, Matsubara H, Matsushita Y, Watanabe M, Vo N, Yoshida H, et al. The Human Bcl-2 Family Member Bcl-rambo Localizes to Mitochondria and Induces Apoptosis and Morphological Aberrations in Drosophila. PLoS ONE. 2016;11:e0157823 pubmed publisher
Slaninova V, Krafcikova M, Perez Gomez R, Steffal P, Trantirek L, Bray S, et al. Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. Open Biol. 2016;6:150155 pubmed publisher
Pinto P, Espinosa Vázquez J, Rivas M, Hombria J. JAK/STAT and Hox Dynamic Interactions in an Organogenetic Gene Cascade. PLoS Genet. 2015;11:e1005412 pubmed publisher
Herboso L, Oliveira M, Talamillo A, Pérez C, González M, Martín D, et al. Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster. Sci Rep. 2015;5:12383 pubmed publisher
Chou M, Hsieh Y, Huang C, Chen P, Chan S, Tsao Y, et al. BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. PLoS ONE. 2015;10:e0130706 pubmed publisher
Troost T, Schneider M, Klein T. A re-examination of the selection of the sensory organ precursor of the bristle sensilla of Drosophila melanogaster. PLoS Genet. 2015;11:e1004911 pubmed publisher
Guo Y, Feng Y, Li Z, Lin X. Drosophila heparan sulfate 3-O sulfotransferase B null mutant is viable and exhibits no defects in Notch signaling. J Genet Genomics. 2014;41:369-78 pubmed publisher
Coumailleau F, Schweisguth F. Insensible is a novel nuclear inhibitor of Notch activity in Drosophila. PLoS ONE. 2014;9:e98213 pubmed publisher
Zacharioudaki E, Bray S. Tools and methods for studying Notch signaling in Drosophila melanogaster. Methods. 2014;68:173-82 pubmed publisher
Fan X, Huang Q, Ye X, Lin Y, Chen Y, Lin X, et al. Drosophila USP5 controls the activation of apoptosis and the Jun N-terminal kinase pathway during eye development. PLoS ONE. 2014;9:e92250 pubmed publisher
Hassel C, Zhang B, Dixon M, Calvi B. Induction of endocycles represses apoptosis independently of differentiation and predisposes cells to genome instability. Development. 2014;141:112-23 pubmed publisher
Sasamura T, Matsuno K, Fortini M. Disruption of Drosophila melanogaster lipid metabolism genes causes tissue overgrowth associated with altered developmental signaling. PLoS Genet. 2013;9:e1003917 pubmed publisher
Babaoglan A, Housden B, Furriols M, Bray S. Deadpan contributes to the robustness of the notch response. PLoS ONE. 2013;8:e75632 pubmed publisher
Baumgartner R, Stocker H, Hafen E. The RNA-binding proteins FMR1, rasputin and caprin act together with the UBA protein lingerer to restrict tissue growth in Drosophila melanogaster. PLoS Genet. 2013;9:e1003598 pubmed publisher
Groth C, Sasamura T, Khanna M, Whitley M, Fortini M. Protein trafficking abnormalities in Drosophila tissues with impaired activity of the ZIP7 zinc transporter Catsup. Development. 2013;140:3018-27 pubmed publisher
Khan S, Bajpai A, Alam M, Gupta R, Harsh S, Pandey R, et al. Epithelial neoplasia in Drosophila entails switch to primitive cell states. Proc Natl Acad Sci U S A. 2013;110:E2163-72 pubmed publisher
Upadhyay A, Kandachar V, Zitserman D, Tong X, Roegiers F. Sanpodo controls sensory organ precursor fate by directing Notch trafficking and binding γ-secretase. J Cell Biol. 2013;201:439-48 pubmed publisher
Fleming R, Hori K, Sen A, Filloramo G, Langer J, Obar R, et al. An extracellular region of Serrate is essential for ligand-induced cis-inhibition of Notch signaling. Development. 2013;140:2039-49 pubmed publisher
Rivas M, Espinosa Vázquez J, Sambrani N, Greig S, Merabet S, Graba Y, et al. Antagonism versus cooperativity with TALE cofactors at the base of the functional diversification of Hox protein function. PLoS Genet. 2013;9:e1003252 pubmed publisher
Denholm B, Hu N, Fauquier T, Caubit X, Fasano L, Skaer H. The tiptop/teashirt genes regulate cell differentiation and renal physiology in Drosophila. Development. 2013;140:1100-10 pubmed publisher
Masoner V, Das R, Pence L, Anand G, LaFerriere H, Zars T, et al. The kinase domain of Drosophila Tribbles is required for turnover of fly C/EBP during cell migration. Dev Biol. 2013;375:33-44 pubmed publisher
Zhang J, Liu M, Su Y, Du J, Zhu A. A targeted in vivo RNAi screen reveals deubiquitinases as new regulators of Notch signaling. G3 (Bethesda). 2012;2:1563-75 pubmed publisher
Dornier E, Coumailleau F, Ottavi J, Moretti J, Boucheix C, Mauduit P, et al. TspanC8 tetraspanins regulate ADAM10/Kuzbanian trafficking and promote Notch activation in flies and mammals. J Cell Biol. 2012;199:481-96 pubmed publisher
Peng Y, Han C, Axelrod J. Planar polarized protrusions break the symmetry of EGFR signaling during Drosophila bract cell fate induction. Dev Cell. 2012;23:507-18 pubmed publisher
Aikin R, Cervantes A, D Angelo G, Ruel L, Lacas Gervais S, Schaub S, et al. A genome-wide RNAi screen identifies regulators of cholesterol-modified hedgehog secretion in Drosophila. PLoS ONE. 2012;7:e33665 pubmed publisher
Couturier L, Vodovar N, Schweisguth F. Endocytosis by Numb breaks Notch symmetry at cytokinesis. Nat Cell Biol. 2012;14:131-9 pubmed publisher
Hori K, Sen A, Kirchhausen T, Artavanis Tsakonas S. Synergy between the ESCRT-III complex and Deltex defines a ligand-independent Notch signal. J Cell Biol. 2011;195:1005-15 pubmed publisher
Sulkowski M, Iyer S, Kurosawa M, Iyer E, Cox D. Turtle functions downstream of Cut in differentially regulating class specific dendrite morphogenesis in Drosophila. PLoS ONE. 2011;6:e22611 pubmed publisher
Tapadia M, Gautam N. Non-apoptotic function of apoptotic proteins in the development of Malpighian tubules of Drosophila melanogaster. J Biosci. 2011;36:531-44 pubmed
Esteve P, Sandonìs A, Cardozo M, Malapeira J, Ibañez C, Crespo I, et al. SFRPs act as negative modulators of ADAM10 to regulate retinal neurogenesis. Nat Neurosci. 2011;14:562-9 pubmed publisher
Yan Y, Denef N, Tang C, Schupbach T. Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling. Development. 2011;138:1697-703 pubmed publisher
Rebeiz M, Miller S, Posakony J. Notch regulates numb: integration of conditional and autonomous cell fate specification. Development. 2011;138:215-25 pubmed publisher
Quijano J, Stinchfield M, Zerlanko B, Gibbens Y, Takaesu N, Hyman Walsh C, et al. The Sno oncogene antagonizes Wingless signaling during wing development in Drosophila. PLoS ONE. 2010;5:e11619 pubmed publisher
Benhra N, Vignaux F, Dussert A, Schweisguth F, Le Borgne R. Neuralized promotes basal to apical transcytosis of delta in epithelial cells. Mol Biol Cell. 2010;21:2078-86 pubmed publisher
Maurel Zaffran C, Pradel J, Graba Y. Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis. Dev Biol. 2010;343:18-27 pubmed publisher
Hamel S, Fantini J, Schweisguth F. Notch ligand activity is modulated by glycosphingolipid membrane composition in Drosophila melanogaster. J Cell Biol. 2010;188:581-94 pubmed publisher
Fiuza U, Klein T, Martinez Arias A, Hayward P. Mechanisms of ligand-mediated inhibition in Notch signaling activity in Drosophila. Dev Dyn. 2010;239:798-805 pubmed publisher
Siddall N, Hime G, Pollock J, Batterham P. Ttk69-dependent repression of lozenge prevents the ectopic development of R7 cells in the Drosophila larval eye disc. BMC Dev Biol. 2009;9:64 pubmed publisher
Steele L, Sukhanova M, Xu J, Gordon G, Huang Y, Yu L, et al. Retinoblastoma family protein promotes normal R8-photoreceptor differentiation in the absence of rhinoceros by inhibiting dE2F1 activity. Dev Biol. 2009;335:228-36 pubmed publisher
Simon F, Fichelson P, Gho M, Audibert A. Notch and Prospero repress proliferation following cyclin E overexpression in the Drosophila bristle lineage. PLoS Genet. 2009;5:e1000594 pubmed publisher
Almudi I, Stocker H, Hafen E, Corominas M, Serras F. SOCS36E specifically interferes with Sevenless signaling during Drosophila eye development. Dev Biol. 2009;326:212-23 pubmed publisher
Kandachar V, Bai T, Chang H. The clathrin-binding motif and the J-domain of Drosophila Auxilin are essential for facilitating Notch ligand endocytosis. BMC Dev Biol. 2008;8:50 pubmed publisher
Wu X, Yamada Mabuchi M, Morris E, Tanwar P, Dobens L, Gluderer S, et al. The Drosophila homolog of human tumor suppressor TSC-22 promotes cellular growth, proliferation, and survival. Proc Natl Acad Sci U S A. 2008;105:5414-9 pubmed publisher
Villa Cuesta E, González Pérez E, Modolell J. Apposition of iroquois expressing and non-expressing cells leads to cell sorting and fold formation in the Drosophila imaginal wing disc. BMC Dev Biol. 2007;7:106 pubmed
Matthews B, Kim M, Flanagan J, Hattori D, Clemens J, Zipursky S, et al. Dendrite self-avoidance is controlled by Dscam. Cell. 2007;129:593-604 pubmed
Jones C, Reifegerste R, Moses K. Characterization of Drosophila mini-me, a gene required for cell proliferation and survival. Genetics. 2006;173:793-808 pubmed
Adamson A, Wright N, LaJeunesse D. Modeling early Epstein-Barr virus infection in Drosophila melanogaster: the BZLF1 protein. Genetics. 2005;171:1125-35 pubmed
Le Borgne R, Remaud S, Hamel S, Schweisguth F. Two distinct E3 ubiquitin ligases have complementary functions in the regulation of delta and serrate signaling in Drosophila. PLoS Biol. 2005;3:e96 pubmed
Mace K, Tugores A. The product of the split ends gene is required for the maintenance of positional information during Drosophila development. BMC Dev Biol. 2004;4:15 pubmed
Mistry H, Wilson B, Roberts I, O Kane C, Skeath J. Cullin-3 regulates pattern formation, external sensory organ development and cell survival during Drosophila development. Mech Dev. 2004;121:1495-507 pubmed
Kamimura K, Rhodes J, Ueda R, McNeely M, Shukla D, Kimata K, et al. Regulation of Notch signaling by Drosophila heparan sulfate 3-O sulfotransferase. J Cell Biol. 2004;166:1069-79 pubmed
Sugimura K, Satoh D, Estes P, Crews S, Uemura T. Development of morphological diversity of dendrites in Drosophila by the BTB-zinc finger protein abrupt. Neuron. 2004;43:809-22 pubmed
Voas M, Rebay I. The novel plant homeodomain protein rhinoceros antagonizes Ras signaling in the Drosophila eye. Genetics. 2003;165:1993-2006 pubmed
Grueber W, Jan L, Jan Y. Different levels of the homeodomain protein cut regulate distinct dendrite branching patterns of Drosophila multidendritic neurons. Cell. 2003;112:805-18 pubmed
Goto S, Taniguchi M, Muraoka M, Toyoda H, Sado Y, Kawakita M, et al. UDP-sugar transporter implicated in glycosylation and processing of Notch. Nat Cell Biol. 2001;3:816-22 pubmed
Nagaso H, Murata T, Day N, Yokoyama K. Simultaneous detection of RNA and protein by in situ hybridization and immunological staining. J Histochem Cytochem. 2001;49:1177-82 pubmed
Lee E, Yu S, Baker N. The scabrous protein can act as an extracellular antagonist of notch signaling in the Drosophila wing. Curr Biol. 2000;10:931-4 pubmed
Jacobsen T, Brennan K, Arias A, Muskavitch M. Cis-interactions between Delta and Notch modulate neurogenic signalling in Drosophila. Development. 1998;125:4531-40 pubmed
Neumann C, Cohen S. A hierarchy of cross-regulation involving Notch, wingless, vestigial and cut organizes the dorsal/ventral axis of the Drosophila wing. Development. 1996;122:3477-85 pubmed
Blochlinger K, Jan L, Jan Y. Postembryonic patterns of expression of cut, a locus regulating sensory organ identity in Drosophila. Development. 1993;117:441-50 pubmed
Blochlinger K, Bodmer R, Jan L, Jan Y. Patterns of expression of cut, a protein required for external sensory organ development in wild-type and cut mutant Drosophila embryos. Genes Dev. 1990;4:1322-31 pubmed
product information
Internal ID :
1547
Name :
2B10
Depositor Name :
Rubin, G.M.
Depositor Institution :
HHMI / Janelia Farm Research
Depositor Website :
http://www.janelia.org/lab/rubin-lab
Date Deposited :
12/19/96
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Cut homeobox
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
1977661
Depositor Notes (Special Instructions) :
This antibody is used to stain the tracheal system.
Collections :
Cell adhesion,Drosophila antigens,Neurodevelopment,Transcription factors
Search Keywords :
Rubin, Gerald M., Cut , Drosophila, MIgG1, Drosophila, Cut, Ct; CT; Cut; CUT; Dmel CG11387; , AB_528186, Monoclonal
Antigen Molecular Weight :
Predicted: 234 kDa
Gene :
Cut
Alternate Gene Name(s) :
Ct; CT; Cut; CUT; Dmel CG11387;
Uniprot ID :
P10180
Antibody Registry ID :
AB_528186
Immunogen :
F2 fusion protein
Immunogen Sequence :
amino acids 1616-1836 of Cut
Clonality :
Monoclonal
Myeloma Strain :
P3x63Ag8.653
Epitope Mapped :
No
Epitope Map PubMed ID :
1977661
Recommended Applications :
Chromatin Immunoprecipitation,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
1977661 8330519 18466624 23091066 10959842 11511686 15511641 23305818 22921201 25064676 12654247 15363392 10959842 23571220 21429988 20410139 19744473 11511686 11533661 9778511 24704358 16079238 21148185 24643212 19662164 20176925 17482551 23708122 23275879 20063416 24086596 21799264 20003234 19083999 15760269 23785054 24244188 21148185 23404107 22267085 14704181 21811639 17482551 21799264 20063416 8951063 23708122 23785054 24244188 21148185 23404107 22267085 14704181 16547096 18375761 24284207 21478884 27095495 31326540 31447264
Immunofluorescence Pubmed IDs :
24902027 20403348 27685172 27696669 29335529 29324767 29530880 27105580 31375669
Western Blot Pubmed IDs :
1977661
Chromatin Immunoprecipitation Pubmed IDs :
21811639
Pubmed IDs :
25064676
DSHB Growth Medium :
Iscove's
References (legacy) :
Genes Dev. 4, 1322-1331.; Development 117, 441-450.; Development 125, 4531-4540.; Curr. Biol. 10, 931-934.; Nature Cell Biol. 3(9), 816-822.; J. Histochem. Cytochem. 49(9), 1177-1182.; Neuron 43, 809-822.; Mech. Dev. 121, 1495-1507.; Dev. Biol. 335, 228-236.; Mole. Biol. Cell 21, 2078-2086.; Dev. Biol. 343, 18-27; Development 138, 1697-1703.; Dev. Cell 23, 507-518.; Dev. Biol. 375, 33-44.; Development 140, 2039-2049.; J. Genet. Genomics 41, 369-378.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US