product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
hexokinase (Type I isozyme)
catalog :
2A4
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2A4
reactivity :
rat
application :
ELISA
more info or order :
citations: 7
| Reference |
|---|
Hashimoto M, Wilson J. Membrane potential-dependent conformational changes in mitochondrially bound hexokinase of brain. Arch Biochem Biophys. 2000;384:163-73 pubmed
|
Finney K, Messer J, DeWitt D, Wilson J. Monoclonal antibodies against rat brain hexokinase. Effects on catalytic function and binding to the outer mitochondrial membrane. J Biol Chem. 1984;259:8232-7 pubmed
|
Ureta T, Smith A, Wilson J. Hexokinase A from mammalian brain: comparative peptide mapping and immunological studies with monoclonal antibodies. Arch Biochem Biophys. 1986;246:419-27 pubmed
|
Smith A, Wilson J. A modified ELISA that selectively detects monoclonal antibodies recognizing native antigen. J Immunol Methods. 1986;94:31-5 pubmed
|
Wilson J, Smith A. Monoclonal antibodies against rat brain hexokinase. Utilization in epitope mapping studies and establishment of structure-function relationships. J Biol Chem. 1985;260:12838-43 pubmed
|
Smith A, Wilson J. Disposition of mitochondrially bound hexokinase at the membrane surface, deduced from reactivity with monoclonal antibodies recognizing epitopes of defined location. Arch Biochem Biophys. 1991;287:359-66 pubmed
|
Smith A, Wilson J. Epitopic regions recognized by monoclonal antibodies against rat brain hexokinase: association with catalytic and regulatory function. Arch Biochem Biophys. 1992;292:165-78 pubmed
|
product information
Internal ID :
1126
Name :
2A4
Depositor Name :
Wilson, J.E.
Depositor Institution :
Michigan State University, Dept. of Biochemistry & Molecular Biology
Date Deposited :
12/10/02
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
hexokinase (Type I isozyme)
Antigen Species :
rat
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Rat
Species Tested (legacy) :
rat
Initial Publication Pubmed ID :
6736033
Depositor Notes (Special Instructions) :
Monoclonal 2A4 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. The epitope recognized is a conformational epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178].
Collections :
Enzymes,Neurodevelopment
Search Keywords :
Wilson, hexokinase (Type I isozyme), hk1, monoclonal, rat, IgG21, ELISA,Function Blocking
Gene :
HK1
Uniprot ID :
P05708
Antibody Registry ID :
AB_528280
Immunogen :
Type I isozyme of rat hexokinase
Clonality :
Monoclonal
Myeloma Strain :
SP2/0-Ag14
Epitope Mapped :
No
Recommended Applications :
ELISA,Function Blocking
Immunoblotting (legacy) :
no
Pubmed IDs :
2413034 3963828 3782816 1716867 1370131 11147827
Additional Information :
The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. The functional effects of this antibody inhibits rat Type I hexokinase activity to >95%, the effect being on V max with no significant effect of Kms for either glucose or ATP [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178. RRID:AB_528280
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Biol. Chem. 259, 8232-8237.; J. Biol. Chem. 260, 12838-12843.; Arch. Biochem. Biophys. 246, 419-427.; J. Immunol. Meth. 94, 31-35.; Arch. Biochem. Biophys. 287, 359-366.; Arch. Biochem. Biophys. 292, 165-178.; Arch. Biochem. Biophys. 384, 163-173.
more info or order :
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