GASP (Gene Analogous to Small Peritrophins) | Developmental Studies Hybridoma Bank 2A12 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

GASP (Gene Analogous to Small Peritrophins)

catalog :

2A12

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A12

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 46

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:2; fig 7j	Hanlon C, Andrew D. Drosophila FoxL1 non-autonomously coordinates organ placement during embryonic development. Dev Biol. 2016;419:273-284 pubmed publisher
	Lin H, Boatz J, Krabbendam I, Kodali R, Hou Z, Wetzel R, et al. Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core. Nat Commun. 2017;8:15462 pubmed publisher
	Sollier K, GaudÃ© H, Chartier F, Laprise P. Rac1 controls epithelial tube length through the apical secretion and polarity pathways. Biol Open. 2015;5:49-54 pubmed publisher
	Boukhatmi H, Schaub C, Bataillé L, Reim I, Frendo J, Frasch M, et al. An Org-1-Tup transcriptional cascade reveals different types of alary muscles connecting internal organs in Drosophila. Development. 2014;141:3761-71 pubmed publisher
	Robbins R, Gbur S, Beitel G. Non-canonical roles for Yorkie and Drosophila Inhibitor of Apoptosis 1 in epithelial tube size control. PLoS ONE. 2014;9:e101609 pubmed publisher
	Iordanou E, Chandran R, Yang Y, Essak M, Blackstone N, Jiang L. The novel Smad protein Expansion regulates the receptor tyrosine kinase pathway to control Drosophila tracheal tube size. Dev Biol. 2014;393:93-108 pubmed publisher
	Dong B, Hannezo E, Hayashi S. Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape. Cell Rep. 2014;7:941-50 pubmed publisher
	Buti E, Mesquita D, Araújo S. Hedgehog is a positive regulator of FGF signalling during embryonic tracheal cell migration. PLoS ONE. 2014;9:e92682 pubmed publisher
	Long S, Fulkerson E, Breese R, Hernández G, Davis C, Melton M, et al. A comparison of midline and tracheal gene regulation during Drosophila development. PLoS ONE. 2014;9:e85518 pubmed publisher
	Tiklová K, Tsarouhas V, Samakovlis C. Control of airway tube diameter and integrity by secreted chitin-binding proteins in Drosophila. PLoS ONE. 2013;8:e67415 pubmed publisher
	Ismat A, Cheshire A, Andrew D. The secreted AdamTS-A metalloprotease is required for collective cell migration. Development. 2013;140:1981-93 pubmed publisher
	Dong B, Kakihara K, Otani T, Wada H, Hayashi S. Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control. Nat Commun. 2013;4:1358 pubmed publisher
	Jaspers M, Nolde K, Behr M, Joo S, Plessmann U, Nikolov M, et al. The claudin Megatrachea protein complex. J Biol Chem. 2012;287:36756-65 pubmed publisher
	Syed Z, Bouge A, Byri S, Chavoshi T, Tång E, Bouhin H, et al. A luminal glycoprotein drives dose-dependent diameter expansion of the Drosophila melanogaster hindgut tube. PLoS Genet. 2012;8:e1002850 pubmed publisher
	Nelson K, Khan Z, Molnar I, Mihaly J, Kaschube M, Beitel G. Drosophila Src regulates anisotropic apical surface growth to control epithelial tube size. Nat Cell Biol. 2012;14:518-25 pubmed publisher
	Sexton T, Yaffe E, Kenigsberg E, Bantignies F, Leblanc B, Hoichman M, et al. Three-dimensional folding and functional organization principles of the Drosophila genome. Cell. 2012;148:458-72 pubmed publisher
	Sakaidani Y, Nomura T, Matsuura A, Ito M, Suzuki E, Murakami K, et al. O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions. Nat Commun. 2011;2:583 pubmed publisher
	Araújo S, Casanova J. Sequoia establishes tip-cell number in Drosophila trachea by regulating FGF levels. J Cell Sci. 2011;124:2335-40 pubmed publisher
	Zhan Y, Maung S, Shao B, Myat M. The bHLH transcription factor, hairy, refines the terminal cell fate in the Drosophila embryonic trachea. PLoS ONE. 2010;5:e14134 pubmed publisher
	Morozova T, Hackett J, Sedaghat Y, Sonnenfeld M. The Drosophila jing gene is a downstream target in the Trachealess/Tango tracheal pathway. Dev Genes Evol. 2010;220:191-206 pubmed publisher
	Tiklová K, Senti K, Wang S, Gräslund A, Samakovlis C. Epithelial septate junction assembly relies on melanotransferrin iron binding and endocytosis in Drosophila. Nat Cell Biol. 2010;12:1071-7 pubmed publisher
	Greenberg L, Hatini V. Systematic expression and loss-of-function analysis defines spatially restricted requirements for Drosophila RhoGEFs and RhoGAPs in leg morphogenesis. Mech Dev. 2011;128:5-17 pubmed publisher
	Laplante C, Paul S, Beitel G, Nilson L. Echinoid regulates tracheal morphology and fusion cell fate in Drosophila. Dev Dyn. 2010;239:2509-19 pubmed publisher
	Nilton A, Oshima K, Zare F, Byri S, Nannmark U, Nyberg K, et al. Crooked, coiled and crimpled are three Ly6-like proteins required for proper localization of septate junction components. Development. 2010;137:2427-37 pubmed publisher
	Norum M, TÃ¥ng E, Chavoshi T, Schwarz H, Linke D, Uv A, et al. Trafficking through COPII stabilises cell polarity and drives secretion during Drosophila epidermal differentiation. PLoS ONE. 2010;5:e10802 pubmed publisher
	Chavoshi T, Moussian B, Uv A. Tissue-autonomous EcR functions are required for concurrent organ morphogenesis in the Drosophila embryo. Mech Dev. 2010;127:308-19 pubmed publisher
	Chanana B, Steigemann P, Jackle H, Vorbrüggen G. Reception of Slit requires only the chondroitin-sulphate-modified extracellular domain of Syndecan at the target cell surface. Proc Natl Acad Sci U S A. 2009;106:11984-8 pubmed publisher
	Massarwa R, Schejter E, Shilo B. Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous. Dev Cell. 2009;16:877-88 pubmed publisher
	Harder B, Schomburg A, Pflanz R, Küstner K, Gerlach N, Schuh R. TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms. Biotechniques. 2008;44:765-72 pubmed publisher
	Jayaram S, Senti K, Tiklová K, Tsarouhas V, Hemphälä J, Samakovlis C. COPI vesicle transport is a common requirement for tube expansion in Drosophila. PLoS ONE. 2008;3:e1964 pubmed publisher
	Tsarouhas V, Senti K, Jayaram S, Tiklová K, Hemphälä J, Adler J, et al. Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila. Dev Cell. 2007;13:214-25 pubmed
	Hsouna A, Lawal H, Izevbaye I, Hsu T, O Donnell J. Drosophila dopamine synthesis pathway genes regulate tracheal morphogenesis. Dev Biol. 2007;308:30-43 pubmed
	Ghabrial A, Krasnow M. Social interactions among epithelial cells during tracheal branching morphogenesis. Nature. 2006;441:746-9 pubmed
	Wang S, Jayaram S, Hemphälä J, Senti K, Tsarouhas V, Jin H, et al. Septate-junction-dependent luminal deposition of chitin deacetylases restricts tube elongation in the Drosophila trachea. Curr Biol. 2006;16:180-5 pubmed
	Merabet S, Ebner A, Affolter M. The Drosophila Extradenticle and Homothorax selector proteins control branchless/FGF expression in mesodermal bridge-cells. EMBO Rep. 2005;6:762-8 pubmed
	Han Z, Li X, Wu J, Olson E. A myocardin-related transcription factor regulates activity of serum response factor in Drosophila. Proc Natl Acad Sci U S A. 2004;101:12567-72 pubmed
	Wu V, Schulte J, Hirschi A, Tepass U, Beitel G. Sinuous is a Drosophila claudin required for septate junction organization and epithelial tube size control. J Cell Biol. 2004;164:313-23 pubmed
	Sedaghat Y, Miranda W, Sonnenfeld M. The jing Zn-finger transcription factor is a mediator of cellular differentiation in the Drosophila CNS midline and trachea. Development. 2002;129:2591-606 pubmed
	Dossenbach C, Rock S, Affolter M. Specificity of FGF signaling in cell migration in Drosophila. Development. 2001;128:4563-72 pubmed
	Boube M, Martin Bermudo M, Brown N, Casanova J. Specific tracheal migration is mediated by complementary expression of cell surface proteins. Genes Dev. 2001;15:1554-62 pubmed
	Chihara T, Hayashi S. Control of tracheal tubulogenesis by Wingless signaling. Development. 2000;127:4433-42 pubmed
	Ikeya T, Hayashi S. Interplay of Notch and FGF signaling restricts cell fate and MAPK activation in the Drosophila trachea. Development. 1999;126:4455-63 pubmed
	Rørth P. Gal4 in the Drosophila female germline. Mech Dev. 1998;78:113-8 pubmed
	Sonnenfeld M, Ward M, Nystrom G, Mosher J, Stahl S, Crews S. The Drosophila tango gene encodes a bHLH-PAS protein that is orthologous to mammalian Arnt and controls CNS midline and tracheal development. Development. 1997;124:4571-82 pubmed
	Samakovlis C, Hacohen N, Manning G, Sutherland D, Guillemin K, Krasnow M. Development of the Drosophila tracheal system occurs by a series of morphologically distinct but genetically coupled branching events. Development. 1996;122:1395-407 pubmed
	Patel N. Imaging neuronal subsets and other cell types in whole-mount Drosophila embryos and larvae using antibody probes. Methods Cell Biol. 1994;44:445-87 pubmed

product information

Internal ID :

2911

Name :

2A12

Depositor Name :

Krasnow, M.A.

Depositor Institution :

Stanford University

Depositor Website :

http://cmgm.stanford.edu/krasnow/

Date Deposited :

11/28/95

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

GASP (Gene Analogous to Small Peritrophins)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7707967

Depositor Notes (Special Instructions) :

2A12 stains from stages 14/15 to 17. Fix the embryos about 20-30 minutes in fix solution described in the initial publication [7707967].

Collections :

Cell markers,Drosophila antigens

Search Keywords :

Krasnow, Mark A., GASP (Gene Analogous to Small Peritrophins) , Drosophila, MIgM, Drosophila, GASP, , AB_528492, Monoclonal

Antigen Molecular Weight :

Predicted: 24 kDa

Gene :

GASP

Uniprot ID :

O97377

Antibody Registry ID :

AB_528492

Clonality :

Monoclonal

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

7707967 22265598 8625828 24651658 11410535 11003842 10498681 17585895 21693579 16760977 9858703 15314239 12015288 18476830 22446737 20935638 19574454 16007069 23826295 25209244 17681133 23536567 22158438 20570942 22930751 20730906 20093179 18398480 16431370 25036253 19531358 9409674 11714681

Immunofluorescence Pubmed IDs :

20851182 21061019 23322046 24794438 24973580 30185423

Western Blot Pubmed IDs :

23826295

Pubmed IDs :

11410535 16760977 24651658 23826295 28537272

Additional Information :

The antigen protein recognized by 2A12 was identified as GASP in 2013 [PMID: 23826295].

DSHB Growth Medium :

Iscove's

References (legacy) :

In "The Development of Drosophila melanogaster" pp. 609-686. Cold Spring Harbor Press.; In "Methods in Cell Biology", Vol. 44: Drosophila melanogaster: practical uses in cell biology. pp. 445-487.; Development 126, 4455-4463.; Development 127, 4433-4442.; Genes Dev. 15, 1554-1562.; Nature 441, 746-749.; PLoS ONE 8(6): e67415. doi:10.1371/journal.pone.0067415.; PLoS ONE 9(3): e92682. doi:10.1371/journal.pone.0092682.

more info or order :

Developmental Studies Hybridoma Bank product webpage