Cubitus interruptus (Ci) full length | Developmental Studies Hybridoma Bank 2A1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Cubitus interruptus (Ci) full length

catalog :

2A1

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

2A1

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:3; fig 5fs1d	Dey N, Ramesh P, Chugh M, Mandal S, Mandal L. Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila. elife. 2016;5: pubmed publisher
immunohistochemistry; fruit fly; 1:20; fig 7p	Zhang Y, Wang X, Matakatsu H, Fehon R, Blair S. The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs. elife. 2016;5: pubmed publisher
immunohistochemistry; fruit fly; 1:250; loading ...; fig 1e	Wang C, Yin M, Wu W, Dong L, Wang S, Lu Y, et al. Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration. Cell Discov. 2016;2:16006 pubmed publisher
immunohistochemistry; fruit fly; loading ...; fig s2a	Jiang K, Liu Y, Fan J, Zhang J, Li X, Evers B, et al. PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail. PLoS Biol. 2016;14:e1002375 pubmed publisher
immunohistochemistry; fruit fly; 1:50; fig 4	Zhou Z, Xu C, Chen P, Liu C, Pang S, Yao X, et al. Stability of HIB-Cul3 E3 ligase adaptor HIB Is Regulated by Self-degradation and Availability of Its Substrates. Sci Rep. 2015;5:12709 pubmed publisher
	Muzzopappa M, Murcia L, Milan M. Feedback amplification loop drives malignant growth in epithelial tissues. Proc Natl Acad Sci U S A. 2017;114:E7291-E7300 pubmed publisher
	Lai C, Lin K, Kao S, Chen Y, Huang F, Hsu H. Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion. J Cell Biol. 2017;216:1439-1453 pubmed publisher
	Bras Pereira C, Potier D, Jacobs J, Aerts S, Casares F, Janody F. dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. PLoS Genet. 2016;12:e1006204 pubmed publisher
	O Keefe L, Lee C, Choo A, Richards R. Tumor Suppressor WWOX Contributes to the Elimination of Tumorigenic Cells in Drosophila melanogaster. PLoS ONE. 2015;10:e0136356 pubmed publisher
	Guo Y, Feng Y, Li Z, Lin X. Drosophila heparan sulfate 3-O sulfotransferase B null mutant is viable and exhibits no defects in Notch signaling. J Genet Genomics. 2014;41:369-78 pubmed publisher
	Herrera S, Morata G. Transgressions of compartment boundaries and cell reprogramming during regeneration in Drosophila. elife. 2014;3:e01831 pubmed publisher
	Clemente Ruiz M, Muzzopappa M, Milan M. Tumor suppressor roles of CENP-E and Nsl1 in Drosophila epithelial tissues. Cell Cycle. 2014;13:1450-5 pubmed publisher
	Zhang J, Du J, Lei C, Liu M, Zhu A. Ubpy controls the stability of the ESCRT-0 subunit Hrs in development. Development. 2014;141:1473-9 pubmed publisher
	Sasamura T, Matsuno K, Fortini M. Disruption of Drosophila melanogaster lipid metabolism genes causes tissue overgrowth associated with altered developmental signaling. PLoS Genet. 2013;9:e1003917 pubmed publisher
	Degoutin J, Milton C, Yu E, Tipping M, Bosveld F, Yang L, et al. Riquiqui and minibrain are regulators of the hippo pathway downstream of Dachsous. Nat Cell Biol. 2013;15:1176-85 pubmed publisher
	Aguilar Hidalgo D, Domínguez Cejudo M, Amore G, Brockmann A, Lemos M, Cordoba A, et al. A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes. Development. 2013;140:82-92 pubmed publisher
	Wu J, Lin W, Chen W, Huang Y, Tang C, Ho M, et al. CSN-mediated deneddylation differentially modulates Ci(155) proteolysis to promote Hedgehog signalling responses. Nat Commun. 2011;2:182 pubmed publisher
	Seong K, Akimaru H, Dai P, Nomura T, Okada M, Ishii S. Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal. PLoS ONE. 2010;5:e15365 pubmed publisher
	Kugler S, Nagel A. A novel Pzg-NURF complex regulates Notch target gene activity. Mol Biol Cell. 2010;21:3443-8 pubmed publisher
	Ruel L, Gallet A, Raisin S, Truchi A, Staccini Lavenant L, Cervantes A, et al. Phosphorylation of the atypical kinesin Costal2 by the kinase Fused induces the partial disassembly of the Smoothened-Fused-Costal2-Cubitus interruptus complex in Hedgehog signalling. Development. 2007;134:3677-89 pubmed
	Dawber R, Hebbes S, Herpers B, Docquier F, van den Heuvel M. Differential range and activity of various forms of the Hedgehog protein. BMC Dev Biol. 2005;5:21 pubmed
	Ogden S, Ascano M, Stegman M, Robbins D. Regulation of Hedgehog signaling: a complex story. Biochem Pharmacol. 2004;67:805-14 pubmed
	Lum L, Zhang C, Oh S, Mann R, von Kessler D, Taipale J, et al. Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2. Mol Cell. 2003;12:1261-74 pubmed
	Hooper J. Smoothened translates Hedgehog levels into distinct responses. Development. 2003;130:3951-63 pubmed
	Noureddine M, Donaldson T, Thacker S, Duronio R. Drosophila Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligase. Dev Cell. 2002;2:757-70 pubmed
	Methot N, Basler K. Hedgehog controls limb development by regulating the activities of distinct transcriptional activator and repressor forms of Cubitus interruptus. Cell. 1999;96:819-31 pubmed
	Ohlmeyer J, Kalderon D. Hedgehog stimulates maturation of Cubitus interruptus into a labile transcriptional activator. Nature. 1998;396:749-53 pubmed
	Aza Blanc P, Ramirez Weber F, Laget M, Schwartz C, Kornberg T. Proteolysis that is inhibited by hedgehog targets Cubitus interruptus protein to the nucleus and converts it to a repressor. Cell. 1997;89:1043-53 pubmed
	Blahovec J. Stress relaxation in cherry fruit. Biorheology. 1996;33:451-62 pubmed
	Motzny C, Holmgren R. The Drosophila cubitus interruptus protein and its role in the wingless and hedgehog signal transduction pathways. Mech Dev. 1995;52:137-50 pubmed

product information

Internal ID :

6233

Name :

2A1

Depositor Name :

Holmgren, R.

Depositor Institution :

Northwestern University

Depositor Website :

http://groups.molbiosci.northwestern.edu/holmgren/

Date Deposited :

9/27/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Cubitus interruptus (Ci) full length

Antigen Species :

Drosophila

Host Species :

rat

Isotype :

RIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7577671

Collections :

Drosophila antigens,Transcription factors

Search Keywords :

Holmgren, Robert, Cubitus interruptus (Ci) full length , Drosophila, RIgG2a, Drosophila, Ci, Ci; ci-D; Ci/Gli; Ci155; ciD; Gli; Siah, AB_2109711, Monoclonal

Antigen Molecular Weight :

Predicted: 155 kDa Apparent:180 kDa,

Gene :

Alternate Gene Name(s) :

Ci; ci-D; Ci/Gli; Ci155; ciD; Gli; Siah

Uniprot ID :

P19538

Antibody Registry ID :

AB_2109711

Immunogen :

Drosophila protein expressed in E. coli and isolated from inclusion bodies.

Clonality :

Monoclonal

Myeloma Strain :

NSO-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

a.a. 700-850 at the C-terminus of the Zn finger domain

Epitope Map PubMed ID :

7577671 9215627

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

7577671 9215627 10102270 21179535 9874371 24626182 24244188 23955303 14636583 12062088 15104233 17881487 21304511 12874118 16197551

Immunofluorescence Pubmed IDs :

23154412 25064676 24755288 24574010 27442438 9093440 29670104 26887950 29844017 31010679

Western Blot Pubmed IDs :

7577671 9215627 14636583

Immunoprecipitation Pubmed IDs :

7577671

Pubmed IDs :

25064676

Additional Information :

Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. The full-length ci form (ciFL), acts as an activator (ciA) while ciR, its C-terminally truncated form, acts as a repressor. Involved in segment polarity. Required for the normal development of the posterior half of each embryonic segment.

DSHB Growth Medium :

Iscove's

References (legacy) :

Mech. Dev. 52, 137-150.; Development 140(1), 82-92.; J. Genet. Genomics 41, 369-378.

more info or order :

Developmental Studies Hybridoma Bank product webpage