product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
chaoptin
catalog :
24B10
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
24B10
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 88
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:20; loading ...; fig 1f
Menon K, Kulkarni V, Takemura S, Anaya M, Zinn K. Interactions between Dpr11 and DIP-γ control selection of amacrine neurons in Drosophila color vision circuits. elife. 2019;8: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; loading ...; fig 8
Cloud V, Thapa A, Morales Sosa P, Miller T, Miller S, Holsapple D, et al. Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization. elife. 2019;8: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:10; fig 1
Ma J, Brennan K, D Aloia M, Pascuzzi P, Weake V. Transcriptome Profiling Identifies Multiplexin as a Target of SAGA Deubiquitinase Activity in Glia Required for Precise Axon Guidance During Drosophila Visual Development. G3 (Bethesda). 2016;6:2435-45 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:200; fig 3C
Lee S, Bang S, Hong Y, Lee J, Jeong H, Park S, et al. The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease. Dis Model Mech. 2016;9:295-306 pubmed publisher
Schlichting M, Weidner P, Diaz M, Menegazzi P, Dalla Benetta E, Helfrich Forster C, et al. Light-Mediated Circuit Switching in the Drosophila Neuronal Clock Network. Curr Biol. 2019;29:3266-3276.e3 pubmed publisher
Plazaola Sasieta H, Zhu Q, Gaitán Peñas H, Rios M, Estévez R, Morey M. Drosophila ClC-a is required in glia of the stem cell niche for proper neurogenesis and wiring of neural circuits. Glia. 2019;67:2374-2398 pubmed publisher
Ojelade S, Lee T, Giagtzoglou N, Yu L, Ugur B, Li Y, et al. cindr, the Drosophila Homolog of the CD2AP Alzheimer's Disease Risk Gene, Is Required for Synaptic Transmission and Proteostasis. Cell Rep. 2019;28:1799-1813.e5 pubmed publisher
Fritsch C, Bernardo Garcia F, Humberg T, Mishra A, Miellet S, Almeida S, et al. Multilevel regulation of the glass locus during Drosophila eye development. PLoS Genet. 2019;15:e1008269 pubmed publisher
Senturk M, Lin G, Zuo Z, Mao D, Watson E, Mikos A, et al. Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidification. Nat Cell Biol. 2019;21:384-396 pubmed publisher
Xu S, Xiao Q, Cosmanescu F, Sergeeva A, Yoo J, Lin Y, et al. Interactions between the Ig-Superfamily Proteins DIP-α and Dpr6/10 Regulate Assembly of Neural Circuits. Neuron. 2018;100:1369-1384.e6 pubmed publisher
Contreras E, Palominos T, Glavic A, Brand A, Sierralta J, Oliva C. The transcription factor SoxD controls neuronal guidance in the Drosophila visual system. Sci Rep. 2018;8:13332 pubmed publisher
Iyer J, Singh M, Jensen M, Patel P, Pizzo L, Huber E, et al. Pervasive genetic interactions modulate neurodevelopmental defects of the autism-associated 16p11.2 deletion in Drosophila melanogaster. Nat Commun. 2018;9:2548 pubmed publisher
Riddiford L, Truman J, Nern A. Juvenile hormone reveals mosaic developmental programs in the metamorphosing optic lobe of Drosophila melanogaster. Biol Open. 2018;7: pubmed publisher
Jin E, Kiral F, Ozel M, Burchardt L, Osterland M, Epstein D, et al. Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals. Curr Biol. 2018;28:1027-1038.e4 pubmed publisher
Peng J, Santiago I, Ahn C, Gur B, Tsui C, Su Z, et al. Drosophila Fezf coordinates laminar-specific connectivity through cell-intrinsic and cell-extrinsic mechanisms. elife. 2018;7: pubmed publisher
Morrison C, Chen H, Cook T, Brown S, Treisman J. Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye. PLoS Genet. 2018;14:e1007173 pubmed publisher
Daniele J, Baqri R, Kunes S. Analysis of axonal trafficking via a novel live-imaging technique reveals distinct hedgehog transport kinetics. Biol Open. 2017;6:714-721 pubmed publisher
Daniele J, Chu T, Kunes S. A novel proteolytic event controls Hedgehog intracellular sorting and distribution to receptive fields. Biol Open. 2017;6:540-550 pubmed publisher
Kremer M, Jung C, Batelli S, Rubin G, Gaul U. The glia of the adult Drosophila nervous system. Glia. 2017;65:606-638 pubmed publisher
Zhou Q, Yu L, Friedrich M, Pignoni F. Distinct regulation of atonal in a visual organ of Drosophila: Organ-specific enhancer and lack of autoregulation in the larval eye. Dev Biol. 2017;421:67-76 pubmed publisher
Liang X, Mahato S, Hemmerich C, Zelhof A. Two temporal functions of Glass: Ommatidium patterning and photoreceptor differentiation. Dev Biol. 2016;414:4-20 pubmed publisher
Ruan W, Srinivasan A, Lin S, Kara k, Barker P. Eiger-induced cell death relies on Rac1-dependent endocytosis. Cell Death Dis. 2016;7:e2181 pubmed publisher
Macpherson L, Zaharieva E, Kearney P, Alpert M, Lin T, Turan Z, et al. Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation. Nat Commun. 2015;6:10024 pubmed publisher
Wernet M, Klovstad M, Clandinin T. A Drosophila toolkit for the visualization and quantification of viral replication launched from transgenic genomes. PLoS ONE. 2014;9:e112092 pubmed publisher
Nie J, Mahato S, Zelhof A. The actomyosin machinery is required for Drosophila retinal lumen formation. PLoS Genet. 2014;10:e1004608 pubmed publisher
Chen X, Squarr A, Stephan R, Chen B, Higgins T, Barry D, et al. Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton. Dev Cell. 2014;30:569-84 pubmed publisher
Lah G, Li J, Millard S. Cell-specific alternative splicing of Drosophila Dscam2 is crucial for proper neuronal wiring. Neuron. 2014;83:1376-88 pubmed publisher
Bertet C, Li X, Erclik T, Cavey M, Wells B, Desplan C. Temporal patterning of neuroblasts controls Notch-mediated cell survival through regulation of Hid or Reaper. Cell. 2014;158:1173-1186 pubmed publisher
Kannan R, Kuzina I, Wincovitch S, Nowotarski S, Giniger E. The Abl/enabled signaling pathway regulates Golgi architecture in Drosophila photoreceptor neurons. Mol Biol Cell. 2014;25:2993-3005 pubmed publisher
Hsieh H, Chang W, Yu L, Rao Y. Control of axon-axon attraction by Semaphorin reverse signaling. Proc Natl Acad Sci U S A. 2014;111:11383-8 pubmed publisher
Bolkan B, Kretzschmar D. Loss of Tau results in defects in photoreceptor development and progressive neuronal degeneration in Drosophila. Dev Neurobiol. 2014;74:1210-25 pubmed publisher
Zschätzsch M, Oliva C, Langen M, De Geest N, Ozel M, Williamson W, et al. Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling. elife. 2014;3:e01699 pubmed publisher
Wernet M, Meier K, Baumann Klausener F, Dorfman R, Weihe U, Labhart T, et al. Genetic dissection of photoreceptor subtype specification by the Drosophila melanogaster zinc finger proteins elbow and no ocelli. PLoS Genet. 2014;10:e1004210 pubmed publisher
Steffensmeier A, Tare M, Puli O, Modi R, Nainaparampil J, Kango Singh M, et al. Novel neuroprotective function of apical-basal polarity gene crumbs in amyloid beta 42 (aβ42) mediated neurodegeneration. PLoS ONE. 2013;8:e78717 pubmed publisher
Shaw J, Chang K. Nebula/DSCR1 upregulation delays neurodegeneration and protects against APP-induced axonal transport defects by restoring calcineurin and GSK-3? signaling. PLoS Genet. 2013;9:e1003792 pubmed publisher
Hein I, Suzuki T, Grunwald Kadow I. Gogo receptor contributes to retinotopic map formation and prevents R1-6 photoreceptor axon bundling. PLoS ONE. 2013;8:e66868 pubmed publisher
Li X, Erclik T, Bertet C, Chen Z, Voutev R, Venkatesh S, et al. Temporal patterning of Drosophila medulla neuroblasts controls neural fates. Nature. 2013;498:456-62 pubmed publisher
Jin M, Jusiak B, Bai Z, Mardon G. Eyes absent tyrosine phosphatase activity is not required for Drosophila development or survival. PLoS ONE. 2013;8:e58818 pubmed publisher
Jusiak B, Abulimiti A, Haelterman N, Chen R, Mardon G. MAPK target sites of eyes absent are not required for eye development or survival in Drosophila. PLoS ONE. 2012;7:e50776 pubmed publisher
Satoh T, Inagaki T, Liu Z, Watanabe R, Satoh A. GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors. Development. 2013;140:385-94 pubmed publisher
Metzger T, Gache V, Xu M, Cadot B, Folker E, Richardson B, et al. MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. Nature. 2012;484:120-4 pubmed publisher
Yano H, Yamamoto Hino M, Awano W, Aoki Kinoshita K, Tsuda Sakurai K, Okano H, et al. Identification of proteasome components required for apical localization of Chaoptin using functional genomics. J Neurogenet. 2012;26:53-63 pubmed publisher
Li Y, Padgett R. bantam is required for optic lobe development and glial cell proliferation. PLoS ONE. 2012;7:e32910 pubmed publisher
Edwards T, Nuschke A, Nern A, Meinertzhagen I. Organization and metamorphosis of glia in the Drosophila visual system. J Comp Neurol. 2012;520:2067-85 pubmed publisher
Dimitroff B, Howe K, Watson A, Campion B, Lee H, Zhao N, et al. Diet and energy-sensing inputs affect TorC1-mediated axon misrouting but not TorC2-directed synapse growth in a Drosophila model of tuberous sclerosis. PLoS ONE. 2012;7:e30722 pubmed publisher
Takemura S, Karuppudurai T, Ting C, Lu Z, Lee C, Meinertzhagen I. Cholinergic circuits integrate neighboring visual signals in a Drosophila motion detection pathway. Curr Biol. 2011;21:2077-84 pubmed publisher
Marrone A, Kucherenko M, Rishko V, Shcherbata H. New dystrophin/dystroglycan interactors control neuron behavior in Drosophila eye. BMC Neurosci. 2011;12:93 pubmed publisher
Stephan R, Gohl C, Fleige A, Klämbt C, Bogdan S. Membrane-targeted WAVE mediates photoreceptor axon targeting in the absence of the WAVE complex in Drosophila. Mol Biol Cell. 2011;22:4079-92 pubmed publisher
Hakeda Suzuki S, Berger Müller S, Tomasi T, Usui T, Horiuchi S, Uemura T, et al. Golden Goal collaborates with Flamingo in conferring synaptic-layer specificity in the visual system. Nat Neurosci. 2011;14:314-23 pubmed publisher
Morante J, Erclik T, Desplan C. Cell migration in Drosophila optic lobe neurons is controlled by eyeless/Pax6. Development. 2011;138:687-93 pubmed publisher
Astigarraga S, Hofmeyer K, Farajian R, Treisman J. Three Drosophila liprins interact to control synapse formation. J Neurosci. 2010;30:15358-68 pubmed publisher
Houalla T, Shi L, van Meyel D, Rao Y. Rab-mediated vesicular transport is required for neuronal positioning in the developing Drosophila visual system. Mol Brain. 2010;3:19 pubmed publisher
Zhan Y, Melian N, Pantoja M, Haines N, Ruohola Baker H, Bourque C, et al. Dystroglycan and mitochondrial ribosomal protein L34 regulate differentiation in the Drosophila eye. PLoS ONE. 2010;5:e10488 pubmed publisher
Walrad P, Hang S, Joseph G, Salas J, Gergen J. Distinct contributions of conserved modules to Runt transcription factor activity. Mol Biol Cell. 2010;21:2315-26 pubmed publisher
Higashi Kovtun M, Mosca T, Dickman D, Meinertzhagen I, Schwarz T. Importin-beta11 regulates synaptic phosphorylated mothers against decapentaplegic, and thereby influences synaptic development and function at the Drosophila neuromuscular junction. J Neurosci. 2010;30:5253-68 pubmed publisher
Malartre M, Ayaz D, Amador F, Martín Bermudo M. The guanine exchange factor vav controls axon growth and guidance during Drosophila development. J Neurosci. 2010;30:2257-67 pubmed publisher
Ferguson K, Long H, Cameron S, Chang W, Rao Y. The conserved Ig superfamily member Turtle mediates axonal tiling in Drosophila. J Neurosci. 2009;29:14151-9 pubmed publisher
Al Anzi B, Wyman R. The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding. Neural Dev. 2009;4:31 pubmed publisher
Zhang Y, Chen D, Wang Z. Analyses of mental dysfunction-related ACSl4 in Drosophila reveal its requirement for Dpp/BMP production and visual wiring in the brain. Hum Mol Genet. 2009;18:3894-905 pubmed publisher
Kanie Y, Yamamoto Hino M, Karino Y, Yokozawa H, Nishihara S, Ueda R, et al. Insight into the regulation of glycan synthesis in Drosophila chaoptin based on mass spectrometry. PLoS ONE. 2009;4:e5434 pubmed publisher
Giagtzoglou N, Lin Y, Haueter C, Bellen H. Importin 13 regulates neurotransmitter release at the Drosophila neuromuscular junction. J Neurosci. 2009;29:5628-39 pubmed publisher
Tamura T, Sone M, Yamashita M, Wanker E, Okazawa H. Glial cell lineage expression of mutant ataxin-1 and huntingtin induces developmental and late-onset neuronal pathologies in Drosophila models. PLoS ONE. 2009;4:e4262 pubmed publisher
Edwards T, Meinertzhagen I. Photoreceptor neurons find new synaptic targets when misdirected by overexpressing runt in Drosophila. J Neurosci. 2009;29:828-41 pubmed publisher
Simpson C, Lemmens R, Miskiewicz K, Broom W, Hansen V, Van Vught P, et al. Variants of the elongator protein 3 (ELP3) gene are associated with motor neuron degeneration. Hum Mol Genet. 2009;18:472-81 pubmed publisher
Petrovic M, Hummel T. Temporal identity in axonal target layer recognition. Nature. 2008;456:800-3 pubmed publisher
Hirai Fujita Y, Yamamoto Hino M, Kanie O, Goto S. N-Glycosylation of the Drosophila neural protein Chaoptin is essential for its stability, cell surface transport and adhesive activity. FEBS Lett. 2008;582:2572-6 pubmed publisher
Kucherenko M, Pantoja M, Yatsenko A, Shcherbata H, Fischer K, Maksymiv D, et al. Genetic modifier screens reveal new components that interact with the Drosophila dystroglycan-dystrophin complex. PLoS ONE. 2008;3:e2418 pubmed publisher
Banerjee S, Bainton R, Mayer N, Beckstead R, Bhat M. Septate junctions are required for ommatidial integrity and blood-eye barrier function in Drosophila. Dev Biol. 2008;317:585-99 pubmed publisher
Morante J, Desplan C. The color-vision circuit in the medulla of Drosophila. Curr Biol. 2008;18:553-65 pubmed publisher
Zhu C, Boone J, Jensen P, Hanna S, Podemski L, Locke J, et al. Drosophila Activin- and the Activin-like product Dawdle function redundantly to regulate proliferation in the larval brain. Development. 2008;135:513-21 pubmed publisher
Sprecher S, Pichaud F, Desplan C. Adult and larval photoreceptors use different mechanisms to specify the same Rhodopsin fates. Genes Dev. 2007;21:2182-95 pubmed
Mindorff E, O Keefe D, Labbe A, Yang J, Ou Y, Yoshikawa S, et al. A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting. Genetics. 2007;176:2247-63 pubmed
Wagner S, Heseding C, Szlachta K, True J, Prinz H, Hovemann B. Drosophila photoreceptors express cysteine peptidase tan. J Comp Neurol. 2007;500:601-11 pubmed
Verdier V, Guang Chao Chen -, Settleman J. Rho-kinase regulates tissue morphogenesis via non-muscle myosin and LIM-kinase during Drosophila development. BMC Dev Biol. 2006;6:38 pubmed
Glater E, Megeath L, Stowers R, Schwarz T. Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent. J Cell Biol. 2006;173:545-57 pubmed
Pramatarova A, Ochalski P, Lee C, Howell B. Mouse disabled 1 regulates the nuclear position of neurons in a Drosophila eye model. Mol Cell Biol. 2006;26:1510-7 pubmed
Umetsu D, Murakami S, Sato M, Tabata T. The highly ordered assembly of retinal axons and their synaptic partners is regulated by Hedgehog/Single-minded in the Drosophila visual system. Development. 2006;133:791-800 pubmed
Long H, Cameron S, Yu L, Rao Y. De novo GMP synthesis is required for axon guidance in Drosophila. Genetics. 2006;172:1633-42 pubmed
Lampel J, Briscoe A, Wasserthal L. Expression of UV-, blue-, long-wavelength-sensitive opsins and melatonin in extraretinal photoreceptors of the optic lobes of hawk moths. Cell Tissue Res. 2005;321:443-58 pubmed
Cafferty P, Yu L, Rao Y. The receptor tyrosine kinase Off-track is required for layer-specific neuronal connectivity in Drosophila. Development. 2004;131:5287-95 pubmed
Takamatsu Y, Nakagoshi H, Rachidi M, Lopes C, Nishida Y, Ohsako S. Characterization of the dCaMKII-GAL4 driver line whose expression is controlled by the Drosophila Ca2+/calmodulin-dependent protein kinase II promoter. Cell Tissue Res. 2002;310:237-52 pubmed
Hiesinger P, Scholz M, Meinertzhagen I, Fischbach K, Obermayer K. Visualization of synaptic markers in the optic neuropils of Drosophila using a new constrained deconvolution method. J Comp Neurol. 2001;429:277-88 pubmed
Fujita S, Zipursky S, Benzer S, Ferrus A, Shotwell S. Monoclonal antibodies against the Drosophila nervous system. Proc Natl Acad Sci U S A. 1982;79:7929-33 pubmed
Zipursky S, Venkatesh T, Teplow D, Benzer S. Neuronal development in the Drosophila retina: monoclonal antibodies as molecular probes. Cell. 1984;36:15-26 pubmed
Zipursky S, Venkatesh T, Benzer S. From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila. Proc Natl Acad Sci U S A. 1985;82:1855-9 pubmed
Reinke R, Krantz D, Yen D, Zipursky S. Chaoptin, a cell surface glycoprotein required for Drosophila photoreceptor cell morphogenesis, contains a repeat motif found in yeast and human. Cell. 1988;52:291-301 pubmed
Van Vactor D, Krantz D, Reinke R, Zipursky S. Analysis of mutants in chaoptin, a photoreceptor cell-specific glycoprotein in Drosophila, reveals its role in cellular morphogenesis. Cell. 1988;52:281-90 pubmed
Krantz D, Zipursky S. Drosophila chaoptin, a member of the leucine-rich repeat family, is a photoreceptor cell-specific adhesion molecule. EMBO J. 1990;9:1969-77 pubmed
product information
Internal ID :
791
Name :
24B10
Depositor Name :
Benzer, S. / Colley, N.
Depositor Institution :
California Institute of Technology
Date Deposited :
3/27/98
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
chaoptin
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila,Insect
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
6818557
Collections :
Cell adhesion,Drosophila antigens,Neurodevelopment
Search Keywords :
Benzer, chaoptin, sensory neurons, mouse, AB_528161, MIgG1, kappa light chain, Monoclonal, Drosophila, Insect, chp, 516; BSH; CG1744; Chaoptin; Chp; CHP; CT5040; Dmel CG1744; mAb 24B10; MAb 24B10; mAb24B10; mAB24B10; Mab24B10; MAb24B10; Nsp, Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Antigen Molecular Weight :
Apparent: 160 kDa
Gene :
chp
Alternate Gene Name(s) :
24B10; 516; BSH; CG1744; Chaoptin; Chp; CHP; CT5040; Dmel CG1744; mAb 24B10; MAb 24B10; mAb24B10; mAB24B10; Mab24B10; MAb24B10; Nsp
Uniprot ID :
P12024
Antibody Registry ID :
AB_528161
Immunogen :
Adult fly heads homogenates
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
Epitope lies within the first 19 aa at the N-terminus
Epitope Map PubMed ID :
6420071 2449286 3920657
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunohistochemistry Pubmed IDs :
11116220 24909306 20392948 2449286 16034628 19158307 6420071 25049408 2189727 25175881 21900504 24755286 25171415 24260128 23250212 23826162 23783517 22351615 22425998 22412948 22137471 21208993 21317905 20540751 20147552 20463973 19617635 21084592 19906964 19403829 16449660 18978776 17603113 18403201 16322525 16882341 18171686 17785526 18407259 16439478 15456725 12397378 31412248
Immunofluorescence Pubmed IDs :
20392948 16034628 18588887 3920657 25203209 21900504 24755286 24260128 23826162 23783517 24086147 22417167 22425998 22412948 22137471 21208993 21317905 20540751 20147552 20463973 21084592 19906964 16449660 18403201 18171686 17785526 18407259 16439478 27693434 29324767 27105580 30190506
Western Blot Pubmed IDs :
6420071 2449286 16034628 3920657 3124963 2189727 25203209
Immunoprecipitation Pubmed IDs :
19415110
Depositor Growth Medium :
15% fetal bovine serum in RPMI 1640 with 1x pen/strep
DSHB Growth Medium :
RPMI
References (legacy) :
Proc. Nati. Acad. Sci. USA 79, 7929-7933.; Cell 36, 15-26.; Cell 52, 281-290.; J. Comp. Neurol. 429, 277-288.; Cell Tissue Res. 321, 443-458.; J. Neurosci. 29(3), 828-841.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US