product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
mannose 6-phosphate receptor, cation-dependent (M6PR)
catalog :
22d4
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
22d4
reactivity :
human, cow
application :
western blot, immunocytochemistry, immunoprecipitation
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:100; loading ...; fig 4e
Wong M, Gillingham A, Munro S. The golgin coiled-coil proteins capture different types of transport carriers via distinct N-terminal motifs. BMC Biol. 2017;15:3 pubmed publisher
Sobajima T, Yoshimura S, Maeda T, Miyata H, Miyoshi E, Harada A. The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution. J Cell Biol. 2018;217:1777-1796 pubmed publisher
Laura R, Dong D, Reynolds W, Maki R. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation. PLoS ONE. 2016;11:e0149391 pubmed publisher
Wong M, Munro S. Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science. 2014;346:1256898 pubmed publisher
Li K, Yang L, Zhang C, Niu Y, Li W, Liu J. HPS6 interacts with dynactin p150Glued to mediate retrograde trafficking and maturation of lysosomes. J Cell Sci. 2014;127:4574-88 pubmed publisher
Porter K, Nallathambi J, Lin Y, Liton P. Lysosomal basification and decreased autophagic flux in oxidatively stressed trabecular meshwork cells: implications for glaucoma pathogenesis. Autophagy. 2013;9:581-94 pubmed publisher
Cepeda V, Esteban M, Fraile Ramos A. Human cytomegalovirus final envelopment on membranes containing both trans-Golgi network and endosomal markers. Cell Microbiol. 2010;12:386-404 pubmed publisher
Joshi A, Nagashima K, Freed E. Defects in cellular sorting and retroviral assembly induced by GGA overexpression. BMC Cell Biol. 2009;10:72 pubmed publisher
Hida T, Ikeda H, Kametaka S, Akazawa C, Kohsaka S, Ebisu S, et al. Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells. Arch Histol Cytol. 2007;70:303-12 pubmed
Stöckli J, Honing S, Rohrer J. The acidic cluster of the CK2 site of the cation-dependent mannose 6-phosphate receptor (CD-MPR) but not its phosphorylation is required for GGA1 and AP-1 binding. J Biol Chem. 2004;279:23542-9 pubmed
Nair P, Schaub B, Rohrer J. Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor. J Biol Chem. 2003;278:24753-8 pubmed
Mathews P, Guerra C, Jiang Y, Grbovic O, Kao B, Schmidt S, et al. Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis. J Biol Chem. 2002;277:5299-307 pubmed
Schweizer A, Stahl P, Rohrer J. A di-aromatic motif in the cytosolic tail of the mannose receptor mediates endosomal sorting. J Biol Chem. 2000;275:29694-700 pubmed
Schweizer A, Kornfeld S, Rohrer J. Proper sorting of the cation-dependent mannose 6-phosphate receptor in endosomes depends on a pair of aromatic amino acids in its cytoplasmic tail. Proc Natl Acad Sci U S A. 1997;94:14471-6 pubmed
Schweizer A, Kornfeld S, Rohrer J. Cysteine34 of the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor is reversibly palmitoylated and required for normal trafficking and lysosomal enzyme sorting. J Cell Biol. 1996;132:577-84 pubmed
Messner D. The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver. Arch Biochem Biophys. 1993;306:391-401 pubmed
Rohrer J, Schweizer A, Johnson K, Kornfeld S. A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes. J Cell Biol. 1995;130:1297-306 pubmed
product information
Internal ID :
3439
Name :
22d4
Depositor Name :
Messner, D.
Depositor Institution :
Bastyr University
Date Deposited :
10/21/04
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
mannose 6-phosphate receptor, cation-dependent (M6PR)
Antigen Species :
bovine
Host Species :
mouse
Isotype :
MIgG2b, kappa light chain
Isotype for catalog (legacy) :
IgG2b, kappa light chain
Positive Tested Species Reactivity :
Bovine,Human
Species Tested (legacy) :
bovine, human
Initial Publication Pubmed ID :
8215441
Depositor Notes (Special Instructions) :
Use non-reducing conditions for western blotting. 22d4 doesn't recognize the murine M6PR.
Collections :
Cell adhesion,Cell signaling,Human
Search Keywords :
Messner, mannose 6-phosphate receptor, cation-dependent, M6PR, CD-MPR, MPR 46, monoclonal, AB_528347, IgG2b, Bovine,Human, Immunofluorescence,Immunoprecipitation,Western Blot
Antigen Molecular Weight :
Predicted: 28 kDa; Apparent: 46 kDa
Gene :
M6PR
Alternate Gene Name(s) :
CD-MPR, MPR 46
Uniprot ID :
P11456
Antibody Registry ID :
AB_528347
Immunogen :
Mannose 6-phosphate receptors mix (MPR) purified from bovine calf liver
Clonality :
Monoclonal
Myeloma Strain :
P3-X63-Ag8.653.3
Epitope Mapped :
Yes
Epitope Location or Sequence :
Extracellular domain
Epitope Map PubMed ID :
11551970
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
8215441 25359980 7559753 12697764 15044437 18431031 25189619 31314175
Western Blot Pubmed IDs :
8215441 26890638 7559753 9405637 11551970 12697764 15044437 10896932 8647889
Immunoprecipitation Pubmed IDs :
8215441 7559753 8647889
Pubmed IDs :
19888988
Additional Information :
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes.
DSHB Growth Medium :
RPMI
References (legacy) :
Arch. Biochem. Biophys. 306(2), 391-401.; J. Cell Biol. 130(6), 1297-1306.; Proc. Natl. Acad. Sci. USA 94(26), 14471-14476.; J. Biol. Chem. 277(7), 5299-5307.; J. Biol. Chem. 278(27), 24753-24758.; Cell. Microbiol. 12(3), 386-404.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US