product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
actin
catalog :
224-236-1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
224-236-1
reactivity :
Dictyostelium discoideum, chicken
application :
western blot, immunocytochemistry
citations: 7
Published Application/Species/Sample/DilutionReference
  • western blot; chicken; 1:2000; fig 5
Zhang S, Li Y, Tan R, Tsoi B, Huang W, Huang Y, et al. A new gestational diabetes mellitus model: hyperglycemia-induced eye malformation via inhibition of Pax6 in the chick embryo. Dis Model Mech. 2016;9:177-86 pubmed publisher
  • western blot; Dictyostelium discoideum; 1:1000; fig 3
Noratel E, Petty C, Kelsey J, Cost H, Basappa N, Blumberg D. The adhesion modulation protein, AmpA localizes to an endocytic compartment and influences substrate adhesion, actin polymerization and endocytosis in vegetative Dictyostelium cells. BMC Cell Biol. 2012;13:29 pubmed publisher
  • immunocytochemistry; Dictyostelium discoideum; 1:1000; fig 7
  • western blot; Dictyostelium discoideum; 1:500; fig 8
Kelsey J, Fastman N, Noratel E, Blumberg D. Ndm, a coiled-coil domain protein that suppresses macropinocytosis and has effects on cell migration. Mol Biol Cell. 2012;23:3407-19 pubmed publisher
CurrĂ  C, McMillan P, Spanos L, Mollard V, Deligianni E, McFadden G, et al. Structured illumination microscopy reveals actin I localization in discreet foci in Plasmodium berghei gametocytes. Exp Parasitol. 2017;181:82-87 pubmed publisher
Charette S, Cosson P. Altered composition and secretion of lysosome-derived compartments in Dictyostelium AP-3 mutant cells. Traffic. 2008;9:588-96 pubmed publisher
Faix J, Clougherty C, Konzok A, Mintert U, Murphy J, Albrecht R, et al. The IQGAP-related protein DGAP1 interacts with Rac and is involved in the modulation of the F-actin cytoskeleton and control of cell motility. J Cell Sci. 1998;111 ( Pt 20):3059-71 pubmed
Westphal M, Jungbluth A, Heidecker M, Muhlbauer B, Heizer C, Schwartz J, et al. Microfilament dynamics during cell movement and chemotaxis monitored using a GFP-actin fusion protein. Curr Biol. 1997;7:176-83 pubmed
product information
Internal ID :
5783
Name :
224-236-1
Depositor Name :
Gerisch, G.
Depositor Institution :
Cell-Dynamics, Max-Plank-Institut fuer Biochemie
Date Deposited :
2/19/09
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
actin
Antigen Species :
Dictyostelium discoideum
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Dictyostelium
Species Tested (legacy) :
Dictyostelium discoideum
Initial Publication Pubmed ID :
9276758
Collections :
Dictyostelium antigens
Search Keywords :
Western Blot Dictyostelium discoideum actin Gerisch, G.
Antigen Molecular Weight :
45 kDa
Antibody Registry ID :
AB_10571933
Immunogen :
actin
Clonality :
Monoclonal
Myeloma Strain :
PAI
Epitope Mapped :
No
Recommended Applications :
Western Blot
Immunoblotting (legacy) :
yes
Western Blot Pubmed IDs :
28803903
Pubmed IDs :
9739079 18194410
Additional Information :
The immunization in 1990 used adjuvant alugel S pertussis. RRID:AB_10571933
DSHB Growth Medium :
Iscove's
References (legacy) :
Curr. Biol. 7, 176-183.; J. Cell Sci. 111, 3059-3071.; Traffic 9, 588-596.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US