product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
blastemal regeneration cell marker of the newt
catalog :
22/18
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
22/18
application :
immunohistochemistry
more info or order :
citations: 5
| Reference |
|---|
Kintner C, Brockes J. Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration. Nature. 1984;308:67-9 pubmed
|
Kintner C, Brockes J. Monoclonal antibodies to the cells of a regenerating limb. J Embryol Exp Morphol. 1985;89:37-55 pubmed
|
Brockes J, Kintner C. Glial growth factor and nerve-dependent proliferation in the regeneration blastema of Urodele amphibians. Cell. 1986;45:301-6 pubmed
|
Fekete D, Brockes J. A monoclonal antibody detects a difference in the cellular composition of developing and regenerating limbs of newts. Development. 1987;99:589-602 pubmed
|
Griffin K, Fekete D, Carlson B. A monoclonal antibody stains myogenic cells in regenerating newt muscle. Development. 1987;101:267-77 pubmed
|
product information
Internal ID :
1346
Name :
22/18
Depositor Name :
Brockes, J.P.
Depositor Institution :
Ludwig Institute for Cancer Research, Middlesex Hospital, University College Branch
Date Deposited :
1/1/87
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
blastemal regeneration cell marker of the newt
Antigen Species :
newt
Host Species :
mouse
Isotype :
MIgM
Isotype for catalog (legacy) :
IgM
Positive Tested Species Reactivity :
Axolotl,Newt
Species Tested (legacy) :
Notopthalmus and Pleurodeles. Axolotl is weak. Triturus is weak. Nothing in higher vertebrates.
Initial Publication Pubmed ID :
6366572
Depositor Notes (Special Instructions) :
Western blot was unsuccessful. For immunohistology see papers of developing and regenerating limbs and normal embryos. The antibody recognizes a cytoplasmic component of a population of blastemal cells that is lost when the cells differentiate into mature tissues. The growth of this population of cells is nerve dependent. (Intermediate filament protein or intermediate filament associated protein when staining fixed cultured blastemal cells.)
Search Keywords :
Immunohistochemistry Axolotl Newt blastemal regeneration cell marker of the newt Brockes, J.P.
Antibody Registry ID :
AB_531815
Immunogen :
early to mid-bud stage blastemas
Clonality :
Monoclonal
Myeloma Strain :
NS1/SP2
Epitope Mapped :
No
Recommended Applications :
Immunohistochemistry
Immunoblotting (legacy) :
unsuccessful
Pubmed IDs :
3912459 3698099 3665773 3446476
Additional Information :
The immunization used 50-100 micrograms of protein for priming and for several boost for sacrificing. The origin of the antibody is notophthalmus viridescens. Works with Notopthalmus and Pleurodeles. Axolotl is weak. Triturus is weak. Nothing in higher vertebrates. RRID:AB_531815
DSHB Growth Medium :
RPMI
References (legacy) :
Nature 308, 67-69.; J. Embryol. Exp. Morph. 89, 37-55.; Cell 45, 301-306.; Development 99, 589-602.; Development 101, 267-277.; J. Tiss. Cult. Meth. 14, 31-36.
more info or order :
company information
Developmental Studies Hybridoma Bank
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