Cdk7, recombinant | Developmental Studies Hybridoma Bank 20H5 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Cdk7, recombinant

catalog :

20H5

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

20H5

application :

western blot, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Seixas C, Cruto T, Tavares A, Gaertig J, Soares H. CCTalpha and CCTdelta chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena. PLoS ONE. 2010;5:e10704 pubmed publisher
Li X, Urwyler O, Suter B. Drosophila Xpd regulates Cdk7 localization, mitotic kinase activity, spindle dynamics, and chromosome segregation. PLoS Genet. 2010;6:e1000876 pubmed publisher
Sharma N, Bryant J, Wloga D, Donaldson R, Davis R, Jerka Dziadosz M, et al. Katanin regulates dynamics of microtubules and biogenesis of motile cilia. J Cell Biol. 2007;178:1065-79 pubmed
Schwartz B, Larochelle S, Suter B, Lis J. Cdk7 is required for full activation of Drosophila heat shock genes and RNA polymerase II phosphorylation in vivo. Mol Cell Biol. 2003;23:6876-86 pubmed
Larochelle S, Chen J, Knights R, Pandur J, Morcillo P, Erdjument Bromage H, et al. T-loop phosphorylation stabilizes the CDK7-cyclin H-MAT1 complex in vivo and regulates its CTD kinase activity. EMBO J. 2001;20:3749-59 pubmed
Larochelle S, Pandur J, Fisher R, Salz H, Suter B. Cdk7 is essential for mitosis and for in vivo Cdk-activating kinase activity. Genes Dev. 1998;12:370-81 pubmed

product information

Internal ID :

8186

Name :

20H5

Depositor Name :

Suter, B.

Depositor Institution :

Institute of Cell Biology, University of Bern, Balzerstrasse

Date Deposited :

4/9/12

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Cdk7, recombinant

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

9450931

Depositor Notes (Special Instructions) :

Fixation of the immunostaining used 4% paraformaldehyde, epitope is methanol sensitive.

Collections :

Drosophila antigens

Search Keywords :

Western Blot Immunoprecipitation Drosophila Cdk7, recombinant Suter, B.

Antigen Molecular Weight :

40 kDa

Antibody Registry ID :

AB_2619579

Immunogen :

Cdk7 (mo15), recombinant Cdk7 expressed in E. coli with TiterMax¨ adjuvant

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Recommended Applications :

Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Pubmed IDs :

11447116 12972606 20300654

Depositor Growth Medium :

IMDM + 10% FBS

Additional Information :

RRID:AB_2619579

DSHB Growth Medium :

Iscove's

References (legacy) :

Genes Dev. 12, 370-381.; EMBO J. 20, 3749-3759.; Mol. Cell Biol. 23(1), 6876-6886.; PLoS Genet. 6(3), e1000876. doi:10.1371/journal.pgen.1000876.

more info or order :

Developmental Studies Hybridoma Bank product webpage