product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Megalin; gp330; Low-density lipoprotein receptor-related protein 2
catalog :
20B megalin
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
20B megalin
reactivity :
rat
application :
ELISA, immunocytochemistry, immunoprecipitation
more info or order :
citations: 10
Reference |
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Nagai M, Meerloo T, Takeda T, Farquhar M. The adaptor protein ARH escorts megalin to and through endosomes. Mol Biol Cell. 2003;14:4984-96 pubmed
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Saito A, Nagai R, Tanuma A, Hama H, Cho K, Takeda T, et al. Role of megalin in endocytosis of advanced glycation end products: implications for a novel protein binding to both megalin and advanced glycation end products. J Am Soc Nephrol. 2003;14:1123-31 pubmed
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Lou X, McQuistan T, Orlando R, Farquhar M. GAIP, GIPC and Galphai3 are concentrated in endocytic compartments of proximal tubule cells: putative role in regulating megalin's function. J Am Soc Nephrol. 2002;13:918-27 pubmed
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Orlando R, Takeda T, Zak B, Schmieder S, Benoit V, McQuistan T, et al. The glomerular epithelial cell anti-adhesin podocalyxin associates with the actin cytoskeleton through interactions with ezrin. J Am Soc Nephrol. 2001;12:1589-98 pubmed
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Rader K, Orlando R, Lou X, Farquhar M. Characterization of ANKRA, a novel ankyrin repeat protein that interacts with the cytoplasmic domain of megalin. J Am Soc Nephrol. 2000;11:2167-78 pubmed
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Orlando R, Rader K, Authier F, Yamazaki H, Posner B, Bergeron J, et al. Megalin is an endocytic receptor for insulin. J Am Soc Nephrol. 1998;9:1759-66 pubmed
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Kerjaschki D, Exner M, Ullrich R, Susani M, Curtiss L, Witztum J, et al. Pathogenic antibodies inhibit the binding of apolipoproteins to megalin/gp330 in passive Heymann nephritis. J Clin Invest. 1997;100:2303-9 pubmed
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Miettinen A, Dekan G, Farquhar M. Monoclonal antibodies against membrane proteins of the rat glomerulus. Immunochemical specificity and immunofluorescence distribution of the antigens. Am J Pathol. 1990;137:929-44 pubmed
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product information
Internal ID :
30412
Name :
20B megalin
Depositor Name :
Farquhar, M.G.
Depositor Institution :
University of California at San Diego
Date Deposited :
2/14/18
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Megalin; gp330; Low-density lipoprotein receptor-related protein 2
Antigen Species :
Rat
Host Species :
mouse
Isotype :
MIgG1
Positive Tested Species Reactivity :
Rat
Initial Publication Pubmed ID :
1699424
Depositor Notes (Special Instructions) :
This antibody recognizes the ectodomain of megalin (gp330) and is useful for endocytic uptake studies.
Search Keywords :
Farquhar, M.G., Megalin; gp330; Low-density lipoprotein receptor-related protein 2, Rat, MIgG1, Rat, Lrp2, , , Monoclonal, , Immunoprecipitation/Immunofluorescence/Function blocking/ELISA
Antigen Molecular Weight :
330 kDa; predicted 519 kDa
Gene :
Lrp2
Uniprot ID :
P98158
Immunogen :
Rat glomeruli membrane extract
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
X63/Ag8.653
Epitope Mapped :
No
Epitope Location or Sequence :
Ectodomain of megalin
Recommended Applications :
ELISA,Function Blocking,Immunofluorescence,Immunoprecipitation
Immunohistochemistry Pubmed IDs :
1699424
Immunofluorescence Pubmed IDs :
1699424 23836931 14528014 11912251 11095640
Immunoprecipitation Pubmed IDs :
1699424 23836931 19340093 9773776 12707383 11461930
ELISA Pubmed IDs :
9410908
Function Blocking Pubmed IDs :
1699424 23836931 14528014
Depositor Growth Medium :
RPMI 1640 with 2mM l-glutamine, hypoxanthine, thymidine plus 10%FCS
DSHB Growth Medium :
Iscove's
more info or order :
company information
Developmental Studies Hybridoma Bank
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