fibronectin, canine, heparin binding region (Hep2) | Developmental Studies Hybridoma Bank 1H9 Fibronectin Hep2 / 1H9B2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

fibronectin, canine, heparin binding region (Hep2)

catalog :

1H9 Fibronectin Hep2 / 1H9B2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H9 Fibronectin Hep2 / 1H9B2

reactivity :

mouse, dog

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 4

Reference
Ben Shoham A, Rot C, Stern T, Krief S, Akiva A, Dadosh T, et al. Deposition of collagen type I onto skeletal endothelium reveals a new role for blood vessels in regulating bone morphology. Development. 2016;143:3933-3943 pubmed
Burton Wurster N, Chen H, Gendelman R, Jackson M, Gagliardo L, Gu D, et al. Specific immunological detection of the (V+C)(-) fibronectin isoform. Matrix Biol. 2002;21:393-8 pubmed
Burton Wurster N, Gendelman R, Chen H, Gu D, Tetreault J, Lust G, et al. The cartilage-specific (V+C)- fibronectin isoform exists primarily in homodimeric and monomeric configurations. Biochem J. 1999;341 ( Pt 3):555-61 pubmed
Burton Wurster N, Lust G. Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma. Arch Biochem Biophys. 1989;269:32-45 pubmed

product information

Internal ID :

1582

Name :

1H9 Fibronectin Hep2 / 1H9B2

Depositor Name :

Burton-Wurster, N.

Depositor Institution :

Baker Institute for Animal Health, College of Veterinary Medicine

Date Deposited :

4/27/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

fibronectin, canine, heparin binding region (Hep2)

Antigen Species :

canine

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Mouse

Species Tested (legacy) :

canine

Initial Publication Pubmed ID :

2916846

Depositor Notes (Special Instructions) :

Specific for canine fibronectin.

Collections :

Extracellular matrix proteins

Search Keywords :

Burton-Wurster, N., fibronectin, canine, heparin binding region (Hep2), FN1, FN, monoclonal, AB_2231928, IgG1, Canine,Mouse, ELISA,Immunofluorescence,Western Blot

Antigen Molecular Weight :

Predicted (multiple isoforms):up to 265 kDa; Apparent: 205, 215, 225 and 230 kDa (multiple isoforms)

Gene :

FN1

Alternate Gene Name(s) :

Uniprot ID :

F1P6H7

Antibody Registry ID :

AB_2231928

Synonyms (Alternate Clone Names) :

1H9A4, 1H9

Immunogen :

Purified canine cartilage fibronectin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP 2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

Type III repeat 14 of canine fibronectin (heparin binding region Hep2)

Epitope Map PubMed ID :

2916846

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

27621060

Western Blot Pubmed IDs :

2916846 10417317

ELISA Pubmed IDs :

2916846

Additional Information :

1H9 was deemed to be canine-specific by the depositor because it doesn't appear to cross-react with human fibronectin. It has since been reported to work on mice fibronectin [PMID: 27621060].

DSHB Growth Medium :

Iscove's

References (legacy) :

Arch. Biochem. Biophys. 269, 32-45.; Biochem. J. 341, 555-561.; Matrix Biol. 21, 393-398.

more info or order :

Developmental Studies Hybridoma Bank product webpage