hindsight: zinc-finger nuclear protein hindsight | Developmental Studies Hybridoma Bank 1G9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

hindsight: zinc-finger nuclear protein hindsight

catalog :

1G9

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1G9

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 42

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:100; loading ...; tbl 3	Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
immunohistochemistry; fruit fly; 1:20; fig 5	Campbell K, Casanova J. A role for E-cadherin in ensuring cohesive migration of a heterogeneous population of non-epithelial cells. Nat Commun. 2015;6:7998 pubmed publisher
	Ewen Campen B, Yang Zhou D, Fernandes V, González D, Liu L, Tao R, et al. Optimized strategy for in vivo Cas9-activation in Drosophila. Proc Natl Acad Sci U S A. 2017;114:9409-9414 pubmed publisher
	Ng B, Selvaraj G, Santa Cruz Mateos C, Grosheva I, Alvarez Garcia I, MartÃn Bermudo M, et al. Î±-Spectrin and integrins act together to regulate actomyosin and columnarization, and to maintain a monolayered follicular epithelium. Development. 2016;143:1388-99 pubmed publisher
	Tognon E, Kobia F, Busi I, Fumagalli A, De Masi F, Vaccari T. Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster. Autophagy. 2016;12:499-514 pubmed publisher
	Troost T, Schneider M, Klein T. A re-examination of the selection of the sensory organ precursor of the bristle sensilla of Drosophila melanogaster. PLoS Genet. 2015;11:e1004911 pubmed publisher
	Jouandin P, Ghiglione C, Noselli S. Starvation induces FoxO-dependent mitotic-to-endocycle switch pausing during Drosophila oogenesis. Development. 2014;141:3013-21 pubmed publisher
	Wisotzkey R, Quijano J, Stinchfield M, Newfeld S. New gene evolution in the bonus-TIF1-?/TRIM33 family impacted the architecture of the vertebrate dorsal-ventral patterning network. Mol Biol Evol. 2014;31:2309-21 pubmed publisher
	Ignesti M, Barraco M, Nallamothu G, Woolworth J, Duchi S, Gargiulo G, et al. Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23. BMC Biol. 2014;12:12 pubmed publisher
	Ming L, Wilk R, Reed B, Lipshitz H. Drosophila Hindsight and mammalian RREB-1 are evolutionarily conserved DNA-binding transcriptional attenuators. Differentiation. 2013;86:159-70 pubmed publisher
	Giagtzoglou N, Li T, Yamamoto S, Bellen H. Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition. J Cell Sci. 2013;126:3686-96 pubmed publisher
	Huang Y, Smith L, Poulton J, Deng W. The microRNA miR-7 regulates Tramtrack69 in a developmental switch in Drosophila follicle cells. Development. 2013;140:897-905 pubmed publisher
	Zhang J, Liu M, Su Y, Du J, Zhu A. A targeted in vivo RNAi screen reveals deubiquitinases as new regulators of Notch signaling. G3 (Bethesda). 2012;2:1563-75 pubmed publisher
	Heck B, Zhang B, Tong X, Pan Z, Deng W, Tsai C. The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophila development. Biol Open. 2012;1:182-96 pubmed publisher
	Schneider M, Troost T, Grawe F, Martinez Arias A, Klein T. Activation of Notch in lgd mutant cells requires the fusion of late endosomes with the lysosome. J Cell Sci. 2013;126:645-56 pubmed publisher
	Stinchfield M, Takaesu N, Quijano J, Castillo A, Tiusanen N, Shimmi O, et al. Fat facets deubiquitylation of Medea/Smad4 modulates interpretation of a Dpp morphogen gradient. Development. 2012;139:2721-9 pubmed publisher
	Domanitskaya E, Schupbach T. CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster. J Cell Sci. 2012;125:399-410 pubmed publisher
	PELUSO C, Umulis D, Kim Y, O Connor M, Serpe M. Shaping BMP morphogen gradients through enzyme-substrate interactions. Dev Cell. 2011;21:375-83 pubmed publisher
	Sun Y, Yan Y, Denef N, Schupbach T. Regulation of somatic myosin activity by protein phosphatase 1β controls Drosophila oocyte polarization. Development. 2011;138:1991-2001 pubmed publisher
	Yan Y, Denef N, Tang C, Schupbach T. Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling. Development. 2011;138:1697-703 pubmed publisher
	Pitsouli C, Perrimon N. Embryonic multipotent progenitors remodel the Drosophila airways during metamorphosis. Development. 2010;137:3615-24 pubmed publisher
	Genevet A, Wehr M, Brain R, Thompson B, Tapon N. Kibra is a regulator of the Salvador/Warts/Hippo signaling network. Dev Cell. 2010;18:300-8 pubmed publisher
	Li Q, Shen L, Xin T, Xiang W, Chen W, Gao Y, et al. Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis. BMC Dev Biol. 2009;9:60 pubmed publisher
	Zúñiga A, Hodar C, Hanna P, Ibáñez F, Moreno P, Pulgar R, et al. Genes encoding novel secreted and transmembrane proteins are temporally and spatially regulated during Drosophila melanogaster embryogenesis. BMC Biol. 2009;7:61 pubmed publisher
	Rajan A, Tien A, Haueter C, Schulze K, Bellen H. The Arp2/3 complex and WASp are required for apical trafficking of Delta into microvilli during cell fate specification of sensory organ precursors. Nat Cell Biol. 2009;11:815-24 pubmed publisher
	Pickup A, Ming L, Lipshitz H. Hindsight modulates Delta expression during Drosophila cone cell induction. Development. 2009;136:975-82 pubmed publisher
	Sun J, Smith L, Armento A, Deng W. Regulation of the endocycle/gene amplification switch by Notch and ecdysone signaling. J Cell Biol. 2008;182:885-96 pubmed publisher
	Vaccari T, Lu H, Kanwar R, Fortini M, Bilder D. Endosomal entry regulates Notch receptor activation in Drosophila melanogaster. J Cell Biol. 2008;180:755-62 pubmed publisher
	Stephan R, Grevelhörster A, Wenderdel S, Klämbt C, Bogdan S. Abi induces ectopic sensory organ formation by stimulating EGFR signaling. Mech Dev. 2008;125:183-95 pubmed publisher
	Fernández B, Arias A, Jacinto A. Dpp signalling orchestrates dorsal closure by regulating cell shape changes both in the amnioserosa and in the epidermis. Mech Dev. 2007;124:884-97 pubmed
	Sun J, Deng W. Hindsight mediates the role of notch in suppressing hedgehog signaling and cell proliferation. Dev Cell. 2007;12:431-42 pubmed
	Hayward P, Balayo T, Martinez Arias A. Notch synergizes with axin to regulate the activity of armadillo in Drosophila. Dev Dyn. 2006;235:2656-66 pubmed
	Reeves N, Posakony J. Genetic programs activated by proneural proteins in the developing Drosophila PNS. Dev Cell. 2005;8:413-25 pubmed
	Reim I, Lee H, Frasch M. The T-box-encoding Dorsocross genes function in amnioserosa development and the patterning of the dorsolateral germ band downstream of Dpp. Development. 2003;130:3187-204 pubmed
	Giraldez A, Copley R, Cohen S. HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient. Dev Cell. 2002;2:667-76 pubmed
	Pickup A, Lamka M, Sun Q, Yip M, Lipshitz H. Control of photoreceptor cell morphology, planar polarity and epithelial integrity during Drosophila eye development. Development. 2002;129:2247-58 pubmed
	Reed B, Wilk R, Lipshitz H. Downregulation of Jun kinase signaling in the amnioserosa is essential for dorsal closure of the Drosophila embryo. Curr Biol. 2001;11:1098-108 pubmed
	Lawrence N, Langdon T, Brennan K, Arias A. Notch signaling targets the Wingless responsiveness of a Ubx visceral mesoderm enhancer in Drosophila. Curr Biol. 2001;11:375-85 pubmed
	Boube M, Llimargas M, Casanova J. Cross-regulatory interactions among tracheal genes support a co-operative model for the induction of tracheal fates in the Drosophila embryo. Mech Dev. 2000;91:271-8 pubmed
	Martin Bermudo M, Alvarez Garcia I, Brown N. Migration of the Drosophila primordial midgut cells requires coordination of diverse PS integrin functions. Development. 1999;126:5161-9 pubmed
	Yip M, Lamka M, Lipshitz H. Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT. Development. 1997;124:2129-41 pubmed
	Dollinger A. [Letter: Idiopathic cyclic edema syndrome]. Nouv Presse Med. 1975;4:1292 pubmed

product information

Internal ID :

3261

Name :

1G9

Depositor Name :

Lipshitz, H.D.

Depositor Institution :

Hospital for Sick Children

Date Deposited :

12/2/97

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

hindsight: zinc-finger nuclear protein hindsight

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila (not grasshopper; other species not tested)

Initial Publication Pubmed ID :

9187140

Depositor Notes (Special Instructions) :

Does not recognize grasshopper hindsight protein

Collections :

Drosophila antigens,Transcription factors

Search Keywords :

Immunofluorescence, Immunohistochemistry, Western Blot, Drosophila hindsight: zinc-finger nuclear protein hindsight; Lipshitz, Howard D., peb, monoclonal, AB_528278, IgG1,Zinc-finger nuclear protein hindsight, CG12212, pebbledanon-4Cg; CG12212; Dmel CG12212; EG:66A1.1; EP55; Hin; Hind; hnd; hnt; Hnt; HNT; Peb; PEB

Antigen Molecular Weight :

Predicted: 209kDa, Apparent: ~250kDa

Gene :

peb

Alternate Gene Name(s) :

Zinc-finger nuclear protein hindsight, CG12212, pebbledanon-4Cg; CG12212; Dmel CG12212; EG:66A1.1; EP55; Hin; Hind; hnd; hnt; Hnt; HNT; Peb; PEB

Uniprot ID :

o46205

Antibody Registry ID :

AB_528278

Immunogen :

GST-Hindsight fusion protein expressed in E. coli

Immunogen Sequence :

aa. 824-1125

Clonality :

Monoclonal

Myeloma Strain :

HL-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 824 to 1125 of Peb/Hnt

Epitope Map PubMed ID :

9187140

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

9187140 24881051 24528630 24993942 21429988 11301248 22745309 20159599 22331351 21490061 20940225 17336908 15737936 19948068 19543274 19772636 10529432 10704851 21839924 19234063 24418439 12015973 1153291 18221859 11959832 11509232 18779369 12783790 23325762 23213409 17950580 31326540

Immunofluorescence Pubmed IDs :

21429988 11301248

Gel Supershift Pubmed IDs :

24418439

Pubmed IDs :

11301248 21429988

Depositor Growth Medium :

15% fetal bovine serum in RPMI 1640 with 1x pen/strep

DSHB Growth Medium :

RPMI

References (legacy) :

Development 124(11), 2129-2141.; Curr. Biol. 11, 375-385.; Development 138, 1697-1703.

more info or order :

Developmental Studies Hybridoma Bank product webpage