neurocan (N-terminal epitope) | Developmental Studies Hybridoma Bank 1F6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

neurocan (N-terminal epitope)

catalog :

1F6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F6

reactivity :

rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; 1:100; loading ...; tbl 1	RÃ¡cz Ã, GaÃ¡l B, Matesz C. Heterogeneous expression of extracellular matrix molecules in the red nucleus of the rat. Neuroscience. 2016;322:1-17 pubmed publisher
immunohistochemistry; rat; 1:100; fig 1	Kecskes S, GaÃ¡l B, RÃ¡cz Ã, Birinyi A, Hunyadi A, Matesz C. Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive. Neuroscience. 2015;284:412-21 pubmed publisher
	Margolis R, Margolis R. Chondroitin sulfate proteoglycans as mediators of axon growth and pathfinding. Cell Tissue Res. 1997;290:343-8 pubmed
	Margolis R, Rauch U, Maurel P, Margolis R. Neurocan and phosphacan: two major nervous tissue-specific chondroitin sulfate proteoglycans. Perspect Dev Neurobiol. 1996;3:273-90 pubmed
	Meyer Puttlitz B, Junker E, Margolis R, Margolis R. Chondroitin sulfate proteoglycans in the developing central nervous system. II. Immunocytochemical localization of neurocan and phosphacan. J Comp Neurol. 1996;366:44-54 pubmed
	Meyer Puttlitz B, Milev P, Junker E, Zimmer I, Margolis R, Margolis R. Chondroitin sulfate and chondroitin/keratan sulfate proteoglycans of nervous tissue: developmental changes of neurocan and phosphacan. J Neurochem. 1995;65:2327-37 pubmed
	Rauch U, Gao P, Janetzko A, Flaccus A, Hilgenberg L, Tekotte H, et al. Isolation and characterization of developmentally regulated chondroitin sulfate and chondroitin/keratan sulfate proteoglycans of brain identified with monoclonal antibodies. J Biol Chem. 1991;266:14785-801 pubmed
	Rauch U, Karthikeyan L, Maurel P, Margolis R, Margolis R. Cloning and primary structure of neurocan, a developmentally regulated, aggregating chondroitin sulfate proteoglycan of brain. J Biol Chem. 1992;267:19536-47 pubmed

product information

Internal ID :

3383

Name :

1F6

Depositor Name :

Margolis, R.K. / Margolis, R.U.

Depositor Institution :

Department of Pharmacology State University of New York

Date Deposited :

1/27/98

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

neurocan (N-terminal epitope)

Antigen Species :

rat

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Rat

Species Tested (legacy) :

rat

Initial Publication Pubmed ID :

1907283

Depositor Notes (Special Instructions) :

For immunocytochemistry have used ascites at dilutions of 1:100-1:500. Hybridoma supernatant is most economical for use with immunoblots.

Collections :

Extracellular matrix proteins,Neurodevelopment

Search Keywords :

Margolis, R.K. / Margolis, R.U., neurocan (N-terminal epitope), mouse, Rat, FFPE/Function Blocking/Immunofluorescence/Immunohistochemistry/Immunoprecipitation/Western Blot, mouse,

Antibody Registry ID :

AB_2149837

Immunogen :

PBS-soluble chondroitin sulfate proteoglycans

Clonality :

Monoclonal

Myeloma Strain :

Designation:PA1

Epitope Mapped :

Recommended Applications :

FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Pubmed IDs :

1326557 7595522 8866845 9117260 9321696

Additional Information :

See Georgadaki and Zagris (2014) Research Journal of Developmental Biology Vol. 1 Article 3. Chmical properties can be found See Rauch et al., 1991, 1992; Meyer-Puttlitz et al., 1995, 1996; Margolis et al., 1996; Margolis and Margolis, 1997. RRID:AB_2149837

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Biol. Chem. 266, 14785-14801.; J. Biol. Chem. 267, 19536-19547.; J. Neurochem. 65, 2327-2337.; J. Comp. Neurol. 366, 44-54.; Perspect. Dev. Neurobiol. 3, 273-290.; Cell Tissue Res. 290, 343-348.; See Georgadaki and Zagris (2014) Research Journal of Developmental Biology Vol. 1 Article 3.

more info or order :

Developmental Studies Hybridoma Bank product webpage