product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
alpha-actinin, smooth muscle-specific
catalog :
1E12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1E12
reactivity :
human, mouse, dog, chicken
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 4
Reference
Radzyukevich T, Neumann J, Rindler T, Oshiro N, Goldhamer D, Lingrel J, et al. Tissue-specific role of the Na,K-ATPase ?2 isozyme in skeletal muscle. J Biol Chem. 2013;288:1226-37 pubmed publisher
Perrot P, Rousseau J, Bouffaut A, Redini F, Cassagnau E, Deschaseaux F, et al. Safety concern between autologous fat graft, mesenchymal stem cell and osteosarcoma recurrence. PLoS ONE. 2010;5:e10999 pubmed publisher
Hungerford J, Hoeffler J, Bowers C, Dahm L, Falchetto R, Shabanowitz J, et al. Identification of a novel marker for primordial smooth muscle and its differential expression pattern in contractile vs noncontractile cells. J Cell Biol. 1997;137:925-37 pubmed
Hungerford J, Owens G, Argraves W, Little C. Development of the aortic vessel wall as defined by vascular smooth muscle and extracellular matrix markers. Dev Biol. 1996;178:375-92 pubmed
product information
Internal ID :
3273
Name :
1E12
Depositor Name :
Little, C.D.
Depositor Institution :
University of Kansas Medical Center
Date Deposited :
9/25/01
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
alpha-actinin, smooth muscle-specific
Antigen Species :
Quail
Host Species :
mouse
Isotype :
MIgM
Isotype for catalog (legacy) :
IgM
Positive Tested Species Reactivity :
Canine,Chicken,Dogfish,Human,Monkey,Mouse,Quail,Shark
Species Tested (legacy) :
quail, human, Chicken, dog, monkey, dogfish shark, mouse
Initial Publication Pubmed ID :
8812136
Depositor Notes (Special Instructions) :
Before western blotting IgM with 0.5% SDS was used. Amino acid sequence analysis is positive and purification is positive.
Collections :
Cytoskeleton,Human
Search Keywords :
Little, C.D., alpha-actinin, smooth muscle-specific, Quail, MIgM, Quail/human/Chicken/dog/Monkey/Dogfish shark/Mouse, ACTN1, , AB_2289065, Monoclonal, Human, Cytoskeleton, Western blot/FFPE/Immunohistochemistry/Immunofluorescence
Antigen Molecular Weight :
103 kDa
Gene :
ACTN1
Uniprot ID :
P12814
Antibody Registry ID :
AB_2289065
Immunogen :
Crude homogenate of embryonic dorsal aorta
Immunogen Sequence :
Full length sequence
Clonality :
Monoclonal
Epitope Mapped :
No
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
8812136 12004079 9151694 10661320 11479726 9264258 12154091 28513320 25631869
Immunofluorescence Pubmed IDs :
23192345 9151694 10397674 12384152 10641596 20544017 12154091
Western Blot Pubmed IDs :
9151694 8812136
Pubmed IDs :
9151694 23192345
Additional Information :
RRID:AB_2289065
DSHB Growth Medium :
Iscove's
References (legacy) :
Dev. Biol. 178(2), 375-392.; J. Cell. Biol. 137(4), 925-937.; J. Biol. Chem. 288(2), 1226-1237.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US