synaptotagmin B | Developmental Studies Hybridoma Bank 1E11 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

synaptotagmin B

catalog :

1E11

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E11

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 3

Reference
Lin M, Mita M, Egertová M, Zampronio C, Jones A, Elphick M. Cellular localization of relaxin-like gonad-stimulating peptide expression in Asterias rubens: New insights into neurohormonal control of spawning in starfish. J Comp Neurol. 2017;525:1599-1617 pubmed publisher
Burke R, Osborne L, Wang D, Murabe N, Yaguchi S, Nakajima Y. Neuron-specific expression of a synaptotagmin gene in the sea urchin Strongylocentrotus purpuratus. J Comp Neurol. 2006;496:244-51 pubmed
Nakajima Y, Kaneko H, Murray G, Burke R. Divergent patterns of neural development in larval echinoids and asteroids. Evol Dev. 2004;6:95-104 pubmed

product information

Internal ID :

8190

Name :

1E11

Depositor Name :

Burke, R.D.

Depositor Institution :

Department of Biochemistry & Microbiology, University of Victoria

Date Deposited :

6/22/12

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

synaptotagmin B

Antigen Species :

Asterina pectinifera

Host Species :

mouse

Isotype :

MIgG3

Isotype for catalog (legacy) :

IgG3

Positive Tested Species Reactivity :

Echinoderm,Invertebrate,Starfish

Species Tested (legacy) :

invertebrate, deuterostomes; ascidian, echinderms, hemichordates

Initial Publication Pubmed ID :

15009122

Depositor Notes (Special Instructions) :

Immunostaining fixation is used ice cold methanol, 4% paraformaldehyde (not longer than 15 minutes). The characterization of the antigen was Mass Spec immunoprecipitated material identify Sp-SynB, mammalian cells expressing SpSynB are immunoreactive, antibodies produced against Sp-SynB have identical patterns ofimmunoreactivity, Burke et al. 2006. Also works with deuterostomes, ascidian, echinderms, hemichordates.

Collections :

Neurodevelopment

Search Keywords :

Burke, R.D., synaptotagmin B, mouse, Invertebrate/Echinoderm , immunoprecipitation/ELISA/Western Blot/Immunofluorescence, mouse,

Antigen Molecular Weight :

65 kDa

Antibody Registry ID :

AB_2617214

Immunogen :

radial nerve lysate

Clonality :

Monoclonal

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

27806429

Pubmed IDs :

16538680

Depositor Growth Medium :

Frozen cells may require supplemented media (ClonaCell Medium E, or 10% EL4B5 PMA stimulated supernatant). 10% FBS DMEM

Additional Information :

We recommend addition of glycerol to 50% and storage at -20C for long term storage. RRID:AB_2617214

DSHB Growth Medium :

Iscove's

References (legacy) :

Evol. Dev. 6, 95-104.; J. Comp. Neurol. 496, 244-251.

more info or order :

Developmental Studies Hybridoma Bank product webpage