fasciclin II (Drosophila) | Developmental Studies Hybridoma Bank 1D4 anti-Fasciclin II product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

fasciclin II (Drosophila)

catalog :

1D4 anti-Fasciclin II

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D4 anti-Fasciclin II

reactivity :

fruit fly, common platanna

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 69

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; fruit fly; 1:100; loading ...; fig 1d	Ashley J, Sorrentino V, Lobb Rabe M, Nagarkar Jaiswal S, Tan L, Xu S, et al. Transsynaptic interactions between IgSF proteins DIP-α and Dpr10 are required for motor neuron targeting specificity. elife. 2019;8: pubmed publisher
immunohistochemistry; fruit fly; 1:20; loading ...; fig 2d	Hakes A, Otsuki L, Brand A. A newly discovered neural stem cell population is generated by the optic lobe neuroepithelium during embryogenesis in Drosophila melanogaster. Development. 2018;145: pubmed publisher
immunohistochemistry - paraffin section; fruit fly; 1:100; fig 2	Chanu S, Sarkar S. Targeted Downregulation of dMyc Suppresses Pathogenesis of Human Neuronal Tauopathies in Drosophila by Limiting Heterochromatin Relaxation and Tau Hyperphosphorylation. Mol Neurobiol. 2017;54:2706-2719 pubmed publisher
immunohistochemistry; fruit fly; 1:100; fig 1	Zwarts L, Goossens T, Clements J, Kang Y, Callaerts P. Axon Branch-Specific Semaphorin-1a Signaling in Drosophila Mushroom Body Development. Front Cell Neurosci. 2016;10:210 pubmed publisher
immunohistochemistry; fruit fly; 1:100; fig 1	Peng Q, Wang Y, Li M, Yuan D, Xu M, Li C, et al. cGMP-Dependent Protein Kinase Encoded by foraging Regulates Motor Axon Guidance in Drosophila by Suppressing Lola Function. J Neurosci. 2016;36:4635-46 pubmed publisher
immunohistochemistry; fruit fly; 1:80; fig s1	Halberg K, Rainey S, Veland I, Neuert H, Dornan A, Klämbt C, et al. The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules. Nat Commun. 2016;7:11266 pubmed publisher
immunohistochemistry; fruit fly; 1:5; fig s4	Carrasco Rando M, Atienza Manuel A, Martin P, Burke R, Ruiz Gomez M. Fear-of-intimacy-mediated zinc transport controls the function of zinc-finger transcription factors involved in myogenesis. Development. 2016;143:1948-57 pubmed publisher
immunohistochemistry - paraffin section; fruit fly; 1:200; fig 2d	Woichansky I, Beretta C, Berns N, Riechmann V. Three mechanisms control E-cadherin localization to the zonula adherens. Nat Commun. 2016;7:10834 pubmed publisher
immunocytochemistry; fruit fly; 1:900; loading ...; fig 6a western blot; fruit fly; 1:100; loading ...; fig 7g	Spring A, Brusich D, Frank C. C-terminal Src Kinase Gates Homeostatic Synaptic Plasticity and Regulates Fasciclin II Expression at the Drosophila Neuromuscular Junction. PLoS Genet. 2016;12:e1005886 pubmed publisher
western blot; fruit fly; 1:50	Marmor Kollet N, Schuldiner O. Contrasting developmental axon regrowth and neurite sprouting of Drosophila mushroom body neurons reveals shared and unique molecular mechanisms. Dev Neurobiol. 2016;76:262-76 pubmed publisher
immunohistochemistry; fruit fly; 1:100	Brown H, Reichert M, Evans T. Slit Binding via the Ig1 Domain Is Essential for Midline Repulsion by Drosophila Robo1 but Dispensable for Receptor Expression, Localization, and Regulation in Vivo. G3 (Bethesda). 2015;5:2429-39 pubmed publisher
immunohistochemistry; fruit fly; 1:100; fig 1	Chance R, Bashaw G. Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion. PLoS Genet. 2015;11:e1005402 pubmed publisher
immunohistochemistry; fruit fly; 1:75; fig 1j	Gresser A, Gutzwiller L, Gauck M, Hartenstein V, Cook T, Gebelein B. Rhomboid Enhancer Activity Defines a Subset of Drosophila Neural Precursors Required for Proper Feeding, Growth and Viability. PLoS ONE. 2015;10:e0134915 pubmed publisher
immunocytochemistry; fruit fly; 1:50	Rohwedder A, Selcho M, Chassot B, Thum A. Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae. J Comp Neurol. 2015;523:2637-64 pubmed publisher
immunohistochemistry; fruit fly; 1:100; fig 2	Evans T, Santiago C, Arbeille E, Bashaw G. Robo2 acts in trans to inhibit Slit-Robo1 repulsion in pre-crossing commissural axons. elife. 2015;4:e08407 pubmed publisher
immunohistochemistry; fruit fly; 1:40	Okumura M, Sakuma C, Miura M, Chihara T. Linking cell surface receptors to microtubules: tubulin folding cofactor D mediates Dscam functions during neuronal morphogenesis. J Neurosci. 2015;35:1979-90 pubmed publisher
immunohistochemistry; fruit fly; 1:15	Marchetti G, Reichardt I, Knoblich J, Besse F. The TRIM-NHL protein Brat promotes axon maintenance by repressing src64B expression. J Neurosci. 2014;34:13855-64 pubmed publisher
immunocytochemistry; fruit fly; 1:20	Li L, Tian X, Zhu M, Bulgari D, Böhme M, Goettfert F, et al. Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons. J Neurosci. 2014;34:8474-87 pubmed publisher
immunohistochemistry; fruit fly; 1:55	Selcho M, Pauls D, Huser A, Stocker R, Thum A. Characterization of the octopaminergic and tyraminergic neurons in the central brain of Drosophila larvae. J Comp Neurol. 2014;522:3485-500 pubmed publisher
immunohistochemistry; fruit fly; 1:50	Apostolopoulou A, Mazija L, Wüst A, Thum A. The neuronal and molecular basis of quinine-dependent bitter taste signaling in Drosophila larvae. Front Behav Neurosci. 2014;8:6 pubmed publisher
immunohistochemistry; common platanna; 1:100 immunohistochemistry; fruit fly; 1:100	Chen J, Condron B. Drosophila serotonergic varicosities are not distributed in a regular manner. J Comp Neurol. 2009;515:441-53 pubmed publisher
	Jantrapirom S, Lo Piccolo L, Yoshida H, Yamaguchi M. A new Drosophila model of Ubiquilin knockdown shows the effect of impaired proteostasis on locomotive and learning abilities. Exp Cell Res. 2018;362:461-471 pubmed publisher
	Baker R, Nakamura N, Chandel I, Howell B, Lyalin D, Panin V. Protein O-Mannosyltransferases Affect Sensory Axon Wiring and Dynamic Chirality of Body Posture in the Drosophila Embryo. J Neurosci. 2018;38:1850-1865 pubmed publisher
	Cheong H, VanBerkum M. Long disordered regions of the C-terminal domain of Abelson tyrosine kinase have specific and additive functions in regulation and axon localization. PLoS ONE. 2017;12:e0189338 pubmed publisher
	Lu C, Zhai B, Mauss A, Landgraf M, Gygi S, Van Vactor D. MicroRNA-8 promotes robust motor axon targeting by coordinate regulation of cell adhesion molecules during synapse development. Philos Trans R Soc Lond B Biol Sci. 2014;369: pubmed publisher
	Kallijärvi J, Stratoulias V, Virtanen K, Hietakangas V, Heino T, Saarma M. Characterization of Drosophila GDNF receptor-like and evidence for its evolutionarily conserved interaction with neural cell adhesion molecule (NCAM)/FasII. PLoS ONE. 2012;7:e51997 pubmed publisher
	Gómez J, Wang Y, Riechmann V. Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis. J Cell Biol. 2012;199:1131-43 pubmed publisher
	Dani N, Nahm M, Lee S, Broadie K. A targeted glycan-related gene screen reveals heparan sulfate proteoglycan sulfation regulates WNT and BMP trans-synaptic signaling. PLoS Genet. 2012;8:e1003031 pubmed publisher
	Selcho M, Pauls D, El Jundi B, Stocker R, Thum A. The role of octopamine and tyramine in Drosophila larval locomotion. J Comp Neurol. 2012;520:3764-85 pubmed publisher
	Beck E, Gasque G, Imlach W, Jiao W, Jiwon Choi B, Wu P, et al. Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag. J Neurosci. 2012;32:7058-73 pubmed publisher
	Kurusu M, Katsuki T, Zinn K, Suzuki E. Developmental changes in expression, subcellular distribution, and function of Drosophila N-cadherin, guided by a cell-intrinsic program during neuronal differentiation. Dev Biol. 2012;366:204-17 pubmed publisher
	Zheng L, Michelson Y, Freger V, Avraham Z, Venken K, Bellen H, et al. Drosophila Ten-m and filamin affect motor neuron growth cone guidance. PLoS ONE. 2011;6:e22956 pubmed publisher
	Sprecher S, Cardona A, Hartenstein V. The Drosophila larval visual system: high-resolution analysis of a simple visual neuropil. Dev Biol. 2011;358:33-43 pubmed publisher
	Goguel V, Belair A, Ayaz D, Lampin Saint Amaux A, Scaplehorn N, Hassan B, et al. Drosophila amyloid precursor protein-like is required for long-term memory. J Neurosci. 2011;31:1032-7 pubmed publisher
	Harumoto T, Ito M, Shimada Y, Kobayashi T, Ueda H, Lu B, et al. Atypical cadherins Dachsous and Fat control dynamics of noncentrosomal microtubules in planar cell polarity. Dev Cell. 2010;19:389-401 pubmed publisher
	Pauls D, Selcho M, Gendre N, Stocker R, Thum A. Drosophila larvae establish appetitive olfactory memories via mushroom body neurons of embryonic origin. J Neurosci. 2010;30:10655-66 pubmed publisher
	Evans I, Hu N, Skaer H, Wood W. Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos. Development. 2010;137:1625-33 pubmed publisher
	Defaye A, Evans I, Crozatier M, Wood W, Lemaitre B, Leulier F. Genetic ablation of Drosophila phagocytes reveals their contribution to both development and resistance to bacterial infection. J Innate Immun. 2009;1:322-34 pubmed publisher
	Kim M, Wen Y, Jan Y. Patterning and organization of motor neuron dendrites in the Drosophila larva. Dev Biol. 2009;336:213-21 pubmed publisher
	Kumar A, Fung S, Lichtneckert R, Reichert H, Hartenstein V. Arborization pattern of engrailed-positive neural lineages reveal neuromere boundaries in the Drosophila brain neuropil. J Comp Neurol. 2009;517:87-104 pubmed publisher
	Chanana B, Steigemann P, Jackle H, Vorbrüggen G. Reception of Slit requires only the chondroitin-sulphate-modified extracellular domain of Syndecan at the target cell surface. Proc Natl Acad Sci U S A. 2009;106:11984-8 pubmed publisher
	Edwards T, Meinertzhagen I. Photoreceptor neurons find new synaptic targets when misdirected by overexpressing runt in Drosophila. J Neurosci. 2009;29:828-41 pubmed publisher
	Lin Y, Reddy B, Irvine K. Requirement for a core 1 galactosyltransferase in the Drosophila nervous system. Dev Dyn. 2008;237:3703-14 pubmed publisher
	Sawaya M, Wojtowicz W, Andre I, Qian B, Wu W, Baker D, et al. A double S shape provides the structural basis for the extraordinary binding specificity of Dscam isoforms. Cell. 2008;134:1007-18 pubmed publisher
	Dorsten J, VanBerkum M. Frazzled cytoplasmic P-motifs are differentially required for axon pathway formation in the Drosophila embryonic CNS. Int J Dev Neurosci. 2008;26:753-61 pubmed publisher
	Daniels R, Gelfand M, Collins C, DiAntonio A. Visualizing glutamatergic cell bodies and synapses in Drosophila larval and adult CNS. J Comp Neurol. 2008;508:131-52 pubmed publisher
	Miller C, Page McCaw A, Broihier H. Matrix metalloproteinases promote motor axon fasciculation in the Drosophila embryo. Development. 2008;135:95-109 pubmed
	Vömel M, Wegener C. Neurotransmitter-induced changes in the intracellular calcium concentration suggest a differential central modulation of CCAP neuron subsets in Drosophila. Dev Neurobiol. 2007;67:792-808 pubmed
	Lee S, Nahm M, Lee M, Kwon M, Kim E, Zadeh A, et al. The F-actin-microtubule crosslinker Shot is a platform for Krasavietz-mediated translational regulation of midline axon repulsion. Development. 2007;134:1767-77 pubmed
	Wang X, Shaw W, Tsang H, Reid E, O Kane C. Drosophila spichthyin inhibits BMP signaling and regulates synaptic growth and axonal microtubules. Nat Neurosci. 2007;10:177-85 pubmed
	Santos J, Pollak E, Rexer K, Molnar L, Wegener C. Morphology and metamorphosis of the peptidergic Va neurons and the median nerve system of the fruit fly, Drosophila melanogaster. Cell Tissue Res. 2006;326:187-99 pubmed
	Araújo S, Aslam H, Tear G, Casanova J. mummy/cystic encodes an enzyme required for chitin and glycan synthesis, involved in trachea, embryonic cuticle and CNS development--analysis of its role in Drosophila tracheal morphogenesis. Dev Biol. 2005;288:179-93 pubmed
	Stewart B, McLean J. Population density regulates Drosophila synaptic morphology in a Fasciclin-II-dependent manner. J Neurobiol. 2004;61:392-9 pubmed
	Michel C, Kraft R, Restifo L. Defective neuronal development in the mushroom bodies of Drosophila fragile X mental retardation 1 mutants. J Neurosci. 2004;24:5798-809 pubmed
	Lee H, Engel U, Rusch J, Scherrer S, Sheard K, Van Vactor D. The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance. Neuron. 2004;42:913-26 pubmed
	Pascual A, Huang K, Neveu J, Preat T. Neuroanatomy: brain asymmetry and long-term memory. Nature. 2004;427:605-6 pubmed
	Rawlins E, Lovegrove B, Jarman A. Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta. Development. 2003;130:6475-84 pubmed
	Mathew D, Popescu A, Budnik V. Drosophila amphiphysin functions during synaptic Fasciclin II membrane cycling. J Neurosci. 2003;23:10710-6 pubmed
	Nassif C, Noveen A, Hartenstein V. Early development of the Drosophila brain: III. The pattern of neuropile founder tracts during the larval period. J Comp Neurol. 2003;455:417-34 pubmed
	Wang J, Zugates C, Liang I, Lee C, Lee T. Drosophila Dscam is required for divergent segregation of sister branches and suppresses ectopic bifurcation of axons. Neuron. 2002;33:559-71 pubmed
	Sharrow M, Tiemeyer M. Gliolectin-mediated carbohydrate binding at the Drosophila midline ensures the fidelity of axon pathfinding. Development. 2001;128:4585-95 pubmed
	Cheng Y, Endo K, Wu K, Rodan A, Heberlein U, Davis R. Drosophila fasciclinII is required for the formation of odor memories and for normal sensitivity to alcohol. Cell. 2001;105:757-68 pubmed
	Paradis S, Sweeney S, Davis G. Homeostatic control of presynaptic release is triggered by postsynaptic membrane depolarization. Neuron. 2001;30:737-49 pubmed
	Hummel T, Krukkert K, Roos J, Davis G, Klämbt C. Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development. Neuron. 2000;26:357-70 pubmed
	Zito K, Parnas D, Fetter R, Isacoff E, Goodman C. Watching a synapse grow: noninvasive confocal imaging of synaptic growth in Drosophila. Neuron. 1999;22:719-29 pubmed
	Davis G, Goodman C. Synapse-specific control of synaptic efficacy at the terminals of a single neuron. Nature. 1998;392:82-6 pubmed
	Zito K, Fetter R, Goodman C, Isacoff E. Synaptic clustering of Fascilin II and Shaker: essential targeting sequences and role of Dlg. Neuron. 1997;19:1007-16 pubmed
	Lin D, Fetter R, Kopczynski C, Grenningloh G, Goodman C. Genetic analysis of Fasciclin II in Drosophila: defasciculation, refasciculation, and altered fasciculation. Neuron. 1994;13:1055-69 pubmed
	Grenningloh G, Rehm E, Goodman C. Genetic analysis of growth cone guidance in Drosophila: fasciclin II functions as a neuronal recognition molecule. Cell. 1991;67:45-57 pubmed

product information

Internal ID :

3532

Name :

1D4 anti-Fasciclin II

Depositor Name :

Goodman, C.

Depositor Institution :

University of California, Berkeley

Date Deposited :

11/1/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

fasciclin II (Drosophila)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

10839355

Collections :

Cell adhesion,Drosophila antigens,Neurodevelopment

Search Keywords :

Goodman, Corey, fasciclin II (Drosophila), mouse, Drosophila, Immunofluorescence/Immunohistochemistry/Western Blot/ELISA, fas2, Ab 1D4; anon-EST:Liang-1.60; CG3665; clone 1.60; CT12301; Dmel CG3665; EG:EG0007.3; Fas; FAS 11; Fas 2; fas II; Fas II; FAS II; fas-II; Fas-II; fas2; FAS2; FascII; fasII; Fasii; FasII; FASII; l(1)G0032; l(1)G0048; l(1)G0081; l(1)G0293; l(1)G0336; mAb 1D4; mAb1D4; mAB1D4; MAB1D4, monoclonal, IgG1, AB_528235

Gene :

Fas2

Alternate Gene Name(s) :

1D4; Ab 1D4; anon-EST:Liang-1.60; CG3665; clone 1.60; CT12301; Dmel CG3665; EG:EG0007.3; Fas; FAS 11; Fas 2; fas II; Fas II; FAS II; fas-II; Fas-II; fas2; FAS2; FascII; fasII; Fasii; FasII; FASII; l(1)G0032; l(1)G0048; l(1)G0081; l(1)G0293; l(1)G0336; mAb 1D4; mAb1D4; mAB1D4; MAB1D4

Uniprot ID :

P34082

Antibody Registry ID :

AB_528235

Clonality :

Monoclonal

Myeloma Strain :

P3 x 63 Ag8.653

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

14627723 21781960 17409115 15207236 29232713 27095495 31327716

Immunofluorescence Pubmed IDs :

17220882 24752702 20702697 22627970 14627656 18985719 16277981 19574454 23144627 21857973 18302156 19711412 18045838 9390515 10230792 23284846 19818341 15490479 14961111 9510251 7946345 23266957 21248128 18805093 18674607 15215302 12508317 11856530 11714683 11430807 29167399 29247619 24752702 26234537 27098704 27095495 30190506 31295251

Western Blot Pubmed IDs :

17220882 24752702 22627970 1913818 19711412 11440718

Pubmed IDs :

25135978 17220882 17443825 19158307 20375589 20392742 20817616 22542600 22593074

Depositor Growth Medium :

15% fetal bovine serum in RPMI 1640 with 1x pen/strep

Additional Information :

For experiments involving live cells and tissue, 1D4 anti-Fasciclin II supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.

DSHB Growth Medium :

RPMI

References (legacy) :

Neuron 26, 357-370.; Cell Tissue Res. 326, 187-199.; Nat. Neurosci. 10(2), 177-185.; Dev. Neurobiol. 67(6), 792-808.; J. Neurosci. 29(3), 828-841.; J. Innate Immun. 1(4), 322-334.; Development 137(10), 1625-1633.; Dev. Cell 19, 389-401.; Dev. Biol. 366, 204-217.; J. Neurosci. 32(20), 7058-7073.; Phil. Trans. R. Soc. B doi:10.1098/rstb.2013.0517.

more info or order :

Developmental Studies Hybridoma Bank product webpage