product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Gurken protein
catalog :
1D12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1D12
reactivity :
Drosophila melanogaster
application :
immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 16
Published Application/Species/Sample/Dilution | Reference |
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Cáceres L, Nilson L. Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte. Development. 2005;132:2345-53 pubmed
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Wilhelm J, Hilton M, Amos Q, Henzel W. Cup is an eIF4E binding protein required for both the translational repression of oskar and the recruitment of Barentsz. J Cell Biol. 2003;163:1197-204 pubmed
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Queenan A, Barcelo G, Van Buskirk C, Schupbach T. The transmembrane region of Gurken is not required for biological activity, but is necessary for transport to the oocyte membrane in Drosophila. Mech Dev. 1999;89:35-42 pubmed
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product information
Internal ID :
2204
Name :
1D12
Depositor Name :
Schupbach, T.
Depositor Institution :
Dept. of Molecular Biology, Princeton University
Date Deposited :
1/25/01
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Gurken protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
10559478
Depositor Notes (Special Instructions) :
The Chemical properties of the antigen are Grk protein : 294 aa, predicted ~33 kDa (but from work with rat polyclonal anti-Grk, appears to
run at 47 kDa).
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Schupbach, T., Gurken protein, mouse, Drosophila, Immunohistochemistry, mouse, a TGF-alpha-like protein
Antigen Molecular Weight :
33 kDa
Antibody Registry ID :
AB_528273
Immunogen :
GST-gurken protein fusion, containing amino acids 53-185 of Grk (extracellular domain)
Clonality :
Monoclonal
Myeloma Strain :
Designation of SP2/0
Epitope Mapped :
No
Recommended Applications :
Immunohistochemistry
Immunoblotting (legacy) :
does not work
Immunofluorescence Pubmed IDs :
27104756 30389853
Pubmed IDs :
15829517 20434988 21429988
Additional Information :
The immunization in 1998 used 50 micrograms plus 50 micrograms plus 100 micrograms plus 25 micrograms. Using intraperitoneal route the adjuvant corixa MPL & TDM emulsion was utilized. RRID:AB_528273
DSHB Growth Medium :
RPMI
References (legacy) :
Mech. Dev. 89, 35-42.; Development 132, 2345-2353.; Cell 141, 509-523.; Development 138, 1697-1703.
more info or order :
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