product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Gurken protein
catalog :
1D12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1D12
reactivity :
Drosophila melanogaster
application :
immunohistochemistry, immunohistochemistry - paraffin section
citations: 16
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:10; fig 7a
Bence M, Jankovics F, Lukacsovich T, Erdelyi M. Combining the auxin-inducible degradation system with CRISPR/Cas9-based genome editing for the conditional depletion of endogenous Drosophila melanogaster proteins. FEBS J. 2017;284:1056-1069 pubmed publisher
  • immunohistochemistry - paraffin section; Drosophila melanogaster; 1:1000; fig s4
Tran M, Tsarouhas V, Kegel A. Early development of Drosophila embryos requires Smc5/6 function during oogenesis. Biol Open. 2016;5:928-41 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:10; fig s1
Williams L, Ganguly S, Loiseau P, Ng B, Palacios I. The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline. Development. 2014;141:176-86 pubmed publisher
Jankovics F, Bence M, Sinka R, Faragó A, Bodai L, Pettkó Szandtner A, et al. Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation. Development. 2018;145: pubmed publisher
Macdonald P, Kanke M, Kenny A. Community effects in regulation of translation. elife. 2016;5:e10965 pubmed publisher
Davidson A, Parton R, Rabouille C, Weil T, Davis I. Localized Translation of gurken/TGF-α mRNA during Axis Specification Is Controlled by Access to Orb/CPEB on Processing Bodies. Cell Rep. 2016;14:2451-62 pubmed publisher
Patil V, Anand A, Chakrabarti A, Kai T. The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster. BMC Biol. 2014;12:61 pubmed publisher
Ott K, Nguyen T, Navarro C. The DExH box helicase domain of spindle-E is necessary for retrotransposon silencing and axial patterning during Drosophila oogenesis. G3 (Bethesda). 2014;4:2247-57 pubmed publisher
Ghosh S, Obrdlik A, Marchand V, Ephrussi A. The EJC binding and dissociating activity of PYM is regulated in Drosophila. PLoS Genet. 2014;10:e1004455 pubmed publisher
Yan Y, Denef N, Tang C, Schupbach T. Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling. Development. 2011;138:1697-703 pubmed publisher
Krahn M, Bückers J, Kastrup L, Wodarz A. Formation of a Bazooka-Stardust complex is essential for plasma membrane polarity in epithelia. J Cell Biol. 2010;190:751-60 pubmed publisher
Morais de Sá E, Mirouse V, St Johnston D. aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells. Cell. 2010;141:509-23 pubmed publisher
Kelkar A, Dobberstein B. Sec61beta, a subunit of the Sec61 protein translocation channel at the endoplasmic reticulum, is involved in the transport of Gurken to the plasma membrane. BMC Cell Biol. 2009;10:11 pubmed publisher
Cáceres L, Nilson L. Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte. Development. 2005;132:2345-53 pubmed
Wilhelm J, Hilton M, Amos Q, Henzel W. Cup is an eIF4E binding protein required for both the translational repression of oskar and the recruitment of Barentsz. J Cell Biol. 2003;163:1197-204 pubmed
Queenan A, Barcelo G, Van Buskirk C, Schupbach T. The transmembrane region of Gurken is not required for biological activity, but is necessary for transport to the oocyte membrane in Drosophila. Mech Dev. 1999;89:35-42 pubmed
product information
Internal ID :
2204
Name :
1D12
Depositor Name :
Schupbach, T.
Depositor Institution :
Dept. of Molecular Biology, Princeton University
Date Deposited :
1/25/01
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Gurken protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
10559478
Depositor Notes (Special Instructions) :
The Chemical properties of the antigen are Grk protein : 294 aa, predicted ~33 kDa (but from work with rat polyclonal anti-Grk, appears to run at 47 kDa).
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Schupbach, T., Gurken protein, mouse, Drosophila, Immunohistochemistry, mouse, a TGF-alpha-like protein
Antigen Molecular Weight :
33 kDa
Antibody Registry ID :
AB_528273
Immunogen :
GST-gurken protein fusion, containing amino acids 53-185 of Grk (extracellular domain)
Clonality :
Monoclonal
Myeloma Strain :
Designation of SP2/0
Epitope Mapped :
No
Recommended Applications :
Immunohistochemistry
Immunoblotting (legacy) :
does not work
Immunofluorescence Pubmed IDs :
27104756 30389853
Pubmed IDs :
15829517 20434988 21429988
Additional Information :
The immunization in 1998 used 50 micrograms plus 50 micrograms plus 100 micrograms plus 25 micrograms. Using intraperitoneal route the adjuvant corixa MPL & TDM emulsion was utilized. RRID:AB_528273
DSHB Growth Medium :
RPMI
References (legacy) :
Mech. Dev. 89, 35-42.; Development 132, 2345-2353.; Cell 141, 509-523.; Development 138, 1697-1703.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US