integrin alphaIIb/beta-3 (CD61) complex, mouse | Developmental Studies Hybridoma Bank 1B5 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin alphaIIb/beta-3 (CD61) complex, mouse

catalog :

1B5

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

1B5

reactivity :

mouse

application :

ELISA, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 9

Reference
Shet A, Hoffmann T, Jirouskova M, Janczak C, Stevens J, Adamson A, et al. Morphological and functional platelet abnormalities in Berkeley sickle cell mice. Blood Cells Mol Dis. 2008;41:109-18 pubmed publisher
Wang Y, Sakuma M, Chen Z, Ustinov V, Shi C, Croce K, et al. Leukocyte engagement of platelet glycoprotein Ibalpha via the integrin Mac-1 is critical for the biological response to vascular injury. Circulation. 2005;112:2993-3000 pubmed
Patel D, Vaananen H, Jirouskova M, Hoffmann T, Bodian C, Coller B. Dynamics of GPIIb/IIIa-mediated platelet-platelet interactions in platelet adhesion/thrombus formation on collagen in vitro as revealed by videomicroscopy. Blood. 2003;101:929-36 pubmed
Eto K, Murphy R, Kerrigan S, Bertoni A, Stuhlmann H, Nakano T, et al. Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling. Proc Natl Acad Sci U S A. 2002;99:12819-24 pubmed
Bertoni A, Tadokoro S, Eto K, Pampori N, Parise L, White G, et al. Relationships between Rap1b, affinity modulation of integrin alpha IIbbeta 3, and the actin cytoskeleton. J Biol Chem. 2002;277:25715-21 pubmed
Zhang Z, Zhang L, Tsang W, Goussev A, Powers C, Ho K, et al. Dynamic platelet accumulation at the site of the occluded middle cerebral artery and in downstream microvessels is associated with loss of microvascular integrity after embolic middle cerebral artery occlusion. Brain Res. 2001;912:181-94 pubmed
Smyth S, Reis E, Vaananen H, Zhang W, Coller B. Variable protection of beta 3-integrin--deficient mice from thrombosis initiated by different mechanisms. Blood. 2001;98:1055-62 pubmed
Smyth S, Tsakiris D, Scudder L, Coller B. Structure and function of murine alphaIIbbeta3 (GPIIb/IIIa): studies using monoclonal antibodies and beta3-null mice,. Thromb Haemost. 2000;84:1103-8 pubmed
Lengweiler S, Smyth S, Jirouskova M, Scudder L, Park H, Moran T, et al. Preparation of monoclonal antibodies to murine platelet glycoprotein IIb/IIIa (alphaIIbbeta3) and other proteins from hamster-mouse interspecies hybridomas. Biochem Biophys Res Commun. 1999;262:167-73 pubmed

product information

Internal ID :

9117

Name :

1B5

Depositor Name :

Coller, B.S.

Depositor Institution :

Laboratory of Blood and Vascular Biology, Rockefeller University

Date Deposited :

5/2/13

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

integrin alphaIIb/beta-3 (CD61) complex, mouse

Antigen Species :

mouse

Host Species :

Armenian hamster

Isotype :

Armenian Hamster IgG

Isotype for catalog (legacy) :

IgG

Positive Tested Species Reactivity :

Mouse

Species Tested (legacy) :

mouse

Initial Publication Pubmed ID :

10448087

Depositor Notes (Special Instructions) :

This antibody doesn't appear to work for western blotting. Doesn't work on Human samples.

Collections :

Cell adhesion

Search Keywords :

Coller, B.S., integrin alphaIIb/beta-3 (CD61) complex, mouse, Itga2b/Itgb3, CD41, monoclonal, mouse, AB_2617390, Armenian hamster IgG, ELISA,FACS,Function Blocking,Immunofluorescence,Immunoprecipitation

Antigen Molecular Weight :

Predicted: 113 kDa (alphaIIb) and 87 kDa (beta3); Apparent: 140 kDa (alphaIIb) 90 kDa (beta3)

Gene :

Itga2b/Itgb3

Alternate Gene Name(s) :

CD41/CD61

Uniprot ID :

Q9QUM0 O54890

Antibody Registry ID :

AB_2617390

Immunogen :

mouse platelets

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0-Ag14

Epitope Mapped :

Recommended Applications :

ELISA,FACS,Function Blocking,Immunofluorescence,Immunoprecipitation

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

11532435

Immunoprecipitation Pubmed IDs :

10448087 11154120

ELISA Pubmed IDs :

10448087

Function Blocking Pubmed IDs :

10448087 12529292 12239348 11493451 16260637 11994301

FACS Pubmed IDs :

10448087 11493451 18374611

Additional Information :

1B5 blocks the alphaIIbbeta3 receptor but not the alphaVbeta3 receptor. In the original publication, the 1B5 isotype was determined to be Armenian hamster IgG3, kappa light chain.

DSHB Growth Medium :

Iscove's

References (legacy) :

Biochem. Biophys. Res. Commun. 262, 167-173.

more info or order :

Developmental Studies Hybridoma Bank product webpage