product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
hexokinase (Type I isozyme)
catalog :
1B2 JW
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1B2 JW
application :
western blot, ELISA
more info or order :
citations: 7
| Reference |
|---|
Hashimoto M, Wilson J. Membrane potential-dependent conformational changes in mitochondrially bound hexokinase of brain. Arch Biochem Biophys. 2000;384:163-73 pubmed
|
Finney K, Messer J, DeWitt D, Wilson J. Monoclonal antibodies against rat brain hexokinase. Effects on catalytic function and binding to the outer mitochondrial membrane. J Biol Chem. 1984;259:8232-7 pubmed
|
Ureta T, Smith A, Wilson J. Hexokinase A from mammalian brain: comparative peptide mapping and immunological studies with monoclonal antibodies. Arch Biochem Biophys. 1986;246:419-27 pubmed
|
Smith A, Wilson J. A modified ELISA that selectively detects monoclonal antibodies recognizing native antigen. J Immunol Methods. 1986;94:31-5 pubmed
|
Wilson J, Smith A. Monoclonal antibodies against rat brain hexokinase. Utilization in epitope mapping studies and establishment of structure-function relationships. J Biol Chem. 1985;260:12838-43 pubmed
|
Smith A, Wilson J. Disposition of mitochondrially bound hexokinase at the membrane surface, deduced from reactivity with monoclonal antibodies recognizing epitopes of defined location. Arch Biochem Biophys. 1991;287:359-66 pubmed
|
Smith A, Wilson J. Epitopic regions recognized by monoclonal antibodies against rat brain hexokinase: association with catalytic and regulatory function. Arch Biochem Biophys. 1992;292:165-78 pubmed
|
product information
Internal ID :
1120
Name :
1B2 JW
Depositor Name :
Wilson, J.E.
Depositor Institution :
301 Biochemistry, Dept. of Biochemistry & Molecular Biology, Michigan State University
Date Deposited :
12/10/02
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
hexokinase (Type I isozyme)
Antigen Species :
rat
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Rat
Species Tested (legacy) :
rat
Initial Publication Pubmed ID :
6736033
Depositor Notes (Special Instructions) :
The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. Binding of the antibody does not affect the kinetic properties of the rat Type I isozyme [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. The epitope recognized is a ÒconformationalÓ (or ÒdiscontinuousÓ) epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. rat; crossreactivity with Type I isozyme from other mammalian species has not been investigated. Monoclonal 1B2 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely.
Collections :
Enzymes
Search Keywords :
Wilson, J.E., hexokinase (Type I isozyme), mouse, Rat , Western Blot/ELISA, mouse,
Antibody Registry ID :
AB_528279
Immunogen :
Type I isozyme of rat hexokinase
Clonality :
Monoclonal
Myeloma Strain :
Designation: Sp2/O-Ag14
Epitope Mapped :
No
Recommended Applications :
ELISA,Western Blot
Immunoblotting (legacy) :
no
Pubmed IDs :
2413034 3963828 3782816 1716867 1370131 11147827
Additional Information :
The origin of the antibody is purified from rat brain. You can find details of the immunization and subsequent fusion protocol are found in Finney et al. (1984) J. Biol. Chem. 259: 8232-8237. Immunization of 100 micrograms of purified rat type I hexokinase was injected intraperitoneally and the adjuvant is freunds. RRID:AB_528279
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Biol. Chem. 259, 8232-8237.; J. Biol. Chem. 260, 12838-12843.; Arch. Biochem. Biophys. 246, 419-427.; J. Immunol. Meth. 94, 31-35.; Arch. Biochem. Biophys. 287, 359-366.; Arch. Biochem. Biophys. 292, 165-178.; Arch. Biochem. Biophys. 384, 163-173.
more info or order :
company information
Developmental Studies Hybridoma Bank