hu-li tai shao, not hts-PC | Developmental Studies Hybridoma Bank 1B1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

hu-li tai shao, not hts-PC

catalog :

1B1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1B1

reactivity :

Drosophila melanogaster

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 56

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; Drosophila melanogaster; loading ...; fig e3b, e7b	Lieber T, Jeedigunta S, Palozzi J, Lehmann R, Hurd T. Mitochondrial fragmentation drives selective removal of deleterious mtDNA in the germline. Nature. 2019;: pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:10; loading ...; fig 1a	Lai C, Lin K, Kao S, Chen Y, Huang F, Hsu H. Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion. J Cell Biol. 2017;216:1439-1453 pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:50; fig 1	Klein J, Qu C, Yang X, Fan Y, Tang C, Peng J. c-Fos Repression by Piwi Regulates Drosophila Ovarian Germline Formation and Tissue Morphogenesis. PLoS Genet. 2016;12:e1006281 pubmed publisher
immunohistochemistry; Drosophila melanogaster	Hashiyama K, Hayashi Y, Kobayashi S. Drosophila Sex lethal gene initiates female development in germline progenitors. Science. 2011;333:885-8 pubmed publisher
	Moon S, Cassani M, Lin Y, Wang L, Dou K, Zhang Z. A Robust Transposon-Endogenizing Response from Germline Stem Cells. Dev Cell. 2018;47:660-671.e3 pubmed publisher
	Jankovics F, Bence M, Sinka R, Faragó A, Bodai L, Pettkó Szandtner A, et al. Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation. Development. 2018;145: pubmed publisher
	Flora P, Schowalter S, Wong Deyrup S, Degennaro M, Nasrallah M, Rangan P. Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila. Dev Biol. 2018;434:84-95 pubmed publisher
	Lu K, Yamashita Y. Germ cell connectivity enhances cell death in response to DNA damage in the Drosophila testis. elife. 2017;6: pubmed publisher
	Tang Y, Geng Q, Chen D, Zhao S, Liu X, Wang Z. Germline Proliferation Is Regulated by Somatic Endocytic Genes via JNK and BMP Signaling in Drosophila. Genetics. 2017;206:189-197 pubmed publisher
	Ma X, Han Y, Song X, Do T, Yang Z, Ni J, et al. DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation. Development. 2016;143:4312-4323 pubmed
	Ameku T, Niwa R. Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila. PLoS Genet. 2016;12:e1006123 pubmed publisher
	Ables E, Hwang G, Finger D, Hinnant T, Drummond Barbosa D. A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis. G3 (Bethesda). 2016;6:2629-42 pubmed publisher
	Macdonald P, Kanke M, Kenny A. Community effects in regulation of translation. elife. 2016;5:e10965 pubmed publisher
	Andress A, Bei Y, Fonslow B, Giri R, Wu Y, Yates J, et al. Spindle-E cycling between nuage and cytoplasm is controlled by Qin and PIWI proteins. J Cell Biol. 2016;213:201-11 pubmed publisher
	Sun J, Wei H, Xu J, Chang J, Yang Z, Ren X, et al. Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation. Nat Commun. 2015;6:8856 pubmed publisher
	Tseng C, Kao S, Wan C, Cho Y, Tung S, Hsu H. Notch signaling mediates the age-associated decrease in adhesion of germline stem cells to the niche. PLoS Genet. 2014;10:e1004888 pubmed publisher
	Matsuoka S, Gupta S, Suzuki E, Hiromi Y, Asaoka M. gone early, a novel germline factor, ensures the proper size of the stem cell precursor pool in the Drosophila ovary. PLoS ONE. 2014;9:e113423 pubmed publisher
	Chen D, Wu C, Zhao S, Geng Q, Gao Y, Li X, et al. Three RNA binding proteins form a complex to promote differentiation of germline stem cell lineage in Drosophila. PLoS Genet. 2014;10:e1004797 pubmed publisher
	Ren X, Yang Z, Mao D, Chang Z, Qiao H, Wang X, et al. Performance of the Cas9 nickase system in Drosophila melanogaster. G3 (Bethesda). 2014;4:1955-62 pubmed publisher
	Herzig B, Yakulov T, Klinge K, GÃ¼nesdogan U, JÃ¤ckle H, Herzig A. BÃ¤llchen is required for self-renewal of germline stem cells in Drosophila melanogaster. Biol Open. 2014;3:510-21 pubmed publisher
	Pokrywka N, Zhang H, Raley Susman K. Distinct roles for hu li tai shao and swallow in cytoskeletal organization during Drosophila oogenesis. Dev Dyn. 2014;243:906-16 pubmed publisher
	König A, Shcherbata H. Visualization of adult stem cells within their niches using the Drosophila germline as a model system. Methods Mol Biol. 2013;1035:25-33 pubmed publisher
	Siddall N, Kalcina M, Johanson T, Monk A, Casagranda F, Been R, et al. Drosophila Rbp6 is an orthologue of vertebrate Msi-1 and Msi-2, but does not function redundantly with dMsi to regulate germline stem cell behaviour. PLoS ONE. 2012;7:e49810 pubmed publisher
	Amoyel M, Sanny J, Burel M, Bach E. Hedgehog is required for CySC self-renewal but does not contribute to the GSC niche in the Drosophila testis. Development. 2013;140:56-65 pubmed publisher
	Morris L, Spradling A. Steroid signaling within Drosophila ovarian epithelial cells sex-specifically modulates early germ cell development and meiotic entry. PLoS ONE. 2012;7:e46109 pubmed publisher
	Goulas S, Conder R, Knoblich J. The Par complex and integrins direct asymmetric cell division in adult intestinal stem cells. Cell Stem Cell. 2012;11:529-40 pubmed publisher
	Rojas Ríos P, Guerrero I, González Reyes A. Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila. PLoS Biol. 2012;10:e1001298 pubmed publisher
	Roth T, Chiang C, Inaba M, Yuan H, Salzmann V, Roth C, et al. Centrosome misorientation mediates slowing of the cell cycle under limited nutrient conditions in Drosophila male germline stem cells. Mol Biol Cell. 2012;23:1524-32 pubmed publisher
	Song Y, Lu B. Regulation of cell growth by Notch signaling and its differential requirement in normal vs. tumor-forming stem cells in Drosophila. Genes Dev. 2011;25:2644-58 pubmed publisher
	Gancz D, Lengil T, Gilboa L. Coordinated regulation of niche and stem cell precursors by hormonal signaling. PLoS Biol. 2011;9:e1001202 pubmed publisher
	Yuan H, Chiang C, Cheng J, Salzmann V, Yamashita Y. Regulation of cyclin A localization downstream of Par-1 function is critical for the centrosome orientation checkpoint in Drosophila male germline stem cells. Dev Biol. 2012;361:57-67 pubmed publisher
	Wang S, Yang J, Tsai A, Kuca T, Sanny J, Lee J, et al. Drosophila adducin regulates Dlg phosphorylation and targeting of Dlg to the synapse and epithelial membrane. Dev Biol. 2011;357:392-403 pubmed publisher
	Pielage J, Bulat V, Zuchero J, Fetter R, Davis G. Hts/Adducin controls synaptic elaboration and elimination. Neuron. 2011;69:1114-31 pubmed publisher
	Ohler S, Hakeda Suzuki S, Suzuki T. Hts, the Drosophila homologue of Adducin, physically interacts with the transmembrane receptor Golden goal to guide photoreceptor axons. Dev Dyn. 2011;240:135-48 pubmed publisher
	Wang Y, Mijares M, Gall M, Turan T, Javier A, Bornemann D, et al. Drosophila variable nurse cells encodes arrest defective 1 (ARD1), the catalytic subunit of the major N-terminal acetyltransferase complex. Dev Dyn. 2010;239:2813-27 pubmed publisher
	Inaba M, Yuan H, Salzmann V, Fuller M, Yamashita Y. E-cadherin is required for centrosome and spindle orientation in Drosophila male germline stem cells. PLoS ONE. 2010;5:e12473 pubmed publisher
	Kugler J, Lem C, Lasko P. Reduced cul-5 activity causes aberrant follicular morphogenesis and germ cell loss in Drosophila oogenesis. PLoS ONE. 2010;5:e9048 pubmed publisher
	Lee K, Yoon J, Park J, Kang Y. Drosophila G9a is implicated in germ cell development. Insect Mol Biol. 2010;19:131-9 pubmed publisher
	Yu J, Reynolds S, Hatfield S, Shcherbata H, Fischer K, Ward E, et al. Dicer-1-dependent Dacapo suppression acts downstream of Insulin receptor in regulating cell division of Drosophila germline stem cells. Development. 2009;136:1497-507 pubmed publisher
	Aruna S, Flores H, Barbash D. Reduced fertility of Drosophila melanogaster hybrid male rescue (Hmr) mutant females is partially complemented by Hmr orthologs from sibling species. Genetics. 2009;181:1437-50 pubmed publisher
	Neumüller R, Betschinger J, Fischer A, Bushati N, Poernbacher I, Mechtler K, et al. Mei-P26 regulates microRNAs and cell growth in the Drosophila ovarian stem cell lineage. Nature. 2008;454:241-5 pubmed publisher
	Lighthouse D, Buszczak M, Spradling A. New components of the Drosophila fusome suggest it plays novel roles in signaling and transport. Dev Biol. 2008;317:59-71 pubmed publisher
	Maines J, Park J, Williams M, McKearin D. Stonewalling Drosophila stem cell differentiation by epigenetic controls. Development. 2007;134:1471-9 pubmed
	Petrella L, Smith Leiker T, Cooley L. The Ovhts polyprotein is cleaved to produce fusome and ring canal proteins required for Drosophila oogenesis. Development. 2007;134:703-12 pubmed
	Terry N, Tulina N, Matunis E, Dinardo S. Novel regulators revealed by profiling Drosophila testis stem cells within their niche. Dev Biol. 2006;294:246-57 pubmed
	Narbonne Reveau K, Besse F, Lamour Isnard C, Busson D, Pret A. fused regulates germline cyst mitosis and differentiation during Drosophila oogenesis. Mech Dev. 2006;123:197-209 pubmed
	Decotto E, Spradling A. The Drosophila ovarian and testis stem cell niches: similar somatic stem cells and signals. Dev Cell. 2005;9:501-10 pubmed
	McNeil G, Smith F, Galioto R. The Drosophila RNA-binding protein Lark is required for the organization of the actin cytoskeleton and Hu-li tai shao localization during oogenesis. Genesis. 2004;40:90-100 pubmed
	Webber H, Howard L, Bickel S. The cohesion protein ORD is required for homologue bias during meiotic recombination. J Cell Biol. 2004;164:819-29 pubmed
	Kauffman T, Tran J, Dinardo S. Mutations in Nop60B, the Drosophila homolog of human dyskeratosis congenita 1, affect the maintenance of the germ-line stem cell lineage during spermatogenesis. Dev Biol. 2003;253:189-99 pubmed
	Cox R, Spradling A. A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis. Development. 2003;130:1579-90 pubmed
	Mathe E, Inoue Y, Palframan W, Brown G, Glover D. Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis. Development. 2003;130:901-15 pubmed
	Baumann O. Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia. Exp Cell Res. 2001;270:176-87 pubmed
	Cox D, Lu B, Sun T, Williams L, Jan Y. Drosophila par-1 is required for oocyte differentiation and microtubule organization. Curr Biol. 2001;11:75-87 pubmed
	Zaccai M, Lipshitz H. Role of Adducin-like (hu-li tai shao) mRNA and protein localization in regulating cytoskeletal structure and function during Drosophila Oogenesis and early embryogenesis. Dev Genet. 1996;19:249-57 pubmed
	Zaccai M, Lipshitz H. Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo. Zygote. 1996;4:159-66 pubmed

product information

Internal ID :

3255

Name :

1B1

Depositor Name :

Lipshitz, H.D.

Depositor Institution :

Hospital for Sick Children

Date Deposited :

12/2/97

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

hu-li tai shao, not hts-PC

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila (other species not tested)

Initial Publication Pubmed ID :

8913030

Depositor Notes (Special Instructions) :

This antigen is also known as adducin-like protein.

Collections :

Drosophila antigens

Search Keywords :

Lipshitz, Howard D., adducin-related protein, Drosophila, hts, Hu-li tai-shao, hts, Immunofluorescence/Immunohistochemistry/Immunoprecipitation/Western Blot, mouse, add; Add-hts; add-like; adducin; hu li tai shao; Ovhts; CG43443, monoclonal, AB_528070, IgG1,

Antigen Molecular Weight :

Multiple molecular weights due to multiple isoforms: the drosophila Adducin-like protein was initially reported to be 87 kDa

Gene :

hts

Alternate Gene Name(s) :

add; Add; Add-hts; add-like; adducin; hu li tai shao; Ovhts; CG43443

Uniprot ID :

Q02645

Antibody Registry ID :

AB_528070

Immunogen :

GST-adducin fusion protein expressed in E. coli

Immunogen Sequence :

aa. 465-903 Of N4 isoform [Uniprot ID Q02645-1]

Clonality :

Monoclonal

Myeloma Strain :

HL-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 472-658 of isoform N4 (see additional information)

Epitope Map PubMed ID :

8913030 11640882

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

11640882 22190460 27091448

Immunofluorescence Pubmed IDs :

12620983 23959979 22131903 23056242 22357619 22024320 20824213 15452872 24677508 23040479 16198292 16616121 12645924 19336466 20140218 23175633 23175633 23209605 18528333 20882681 11231123 16516445 19153254 12538517 17344229 18355804 24876388 30393075 28809158 27104756 30389853

Western Blot Pubmed IDs :

8913030 21128303 17215303 21791202 21435557 20002223

Immunoprecipitation Pubmed IDs :

21791202

Pubmed IDs :

8913030 8952067 11640882

Depositor Growth Medium :

15% fetal bovine serum in RPMI 1640 with 1x pen/strep

Additional Information :

1B1 recognizes all isoforms produced by alternative splicing except ShAdd (also known as hts-PC or N32/R2 [Uniprot ID Q02645-3]). By deduction, the 1B1 epitope is located in the region of N4 [Uniprot ID Q02645-1] common to all isoforms but absent in ShAdd, and corresponds to aa 472-658 of isoform N4. 1B1 recognizes only one fragment resulting from proteolysis of Ovhts (N4): Ovhts Fus is recognized but Ovhts-RC is not.

DSHB Growth Medium :

RPMI

References (legacy) :

Proc. Natl. Acad. Sci. USA 90, 2512-2516.; Zygote 4, 159-166.; Dev. Genet. 19, 249-257.; Exp. Cell Res. 270, 176-187.

more info or order :

Developmental Studies Hybridoma Bank product webpage