product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
ecdysone receptor (EcR) (common), Manduca sexta
catalog :
15C3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
15C3
application :
western blot, immunohistochemistry
more info or order :
citations: 2
| Reference |
|---|
Jindra M, Malone F, Hiruma K, Riddiford L. Developmental profiles and ecdysteroid regulation of the mRNAs for two ecdysone receptor isoforms in the epidermis and wings of the tobacco hornworm, Manduca sexta. Dev Biol. 1996;180:258-72 pubmed
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Fujiwara H, Jindra M, Newitt R, Palli S, Hiruma K, Riddiford L. Cloning of an ecdysone receptor homolog from Manduca sexta and the developmental profile of its mRNA in wings. Insect Biochem Mol Biol. 1995;25:845-56 pubmed
|
product information
Internal ID :
1517
Name :
15C3
Depositor Name :
Riddiford, L.M.
Depositor Institution :
Department of Zoology, University of Washington
Date Deposited :
2/11/98
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
ecdysone receptor (EcR) (common), Manduca sexta
Antigen Species :
Manduca sexta
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Bombyx mori,Drosophila,Locusta migratoria,Manduca
Species Tested (legacy) :
Manduca; Bombyx mori; Locusta migratoria (?); Drosophila melanogaster
Initial Publication Pubmed ID :
7633469
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Riddiford, L.M., ecdysone receptor (EcR) (common), Manduca sexta, MIgG2b, Manduca/Bombyx mori/Locusta Migratoria/drosophila, Monoclonal, Manduca sexta, Western Blot/Immunohistochemistry
Antigen Molecular Weight :
64 kDa (predicted); between 70-80 kDa by immunoblotting
Antibody Registry ID :
AB_528211
Immunogen :
Manduca ecdysone receptor, only the EcR-B1 portion of the fusion protein injected
Clonality :
Monoclonal
Myeloma Strain :
Designation of Fox-NY cells
Epitope Mapped :
No
Recommended Applications :
Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Pubmed IDs :
8948589
Additional Information :
Immunization of 100 micrograms on 3/10/95, 4/20/95, 5/3/95, 5/30/95 used complete Freund’s adjuvant (1st); boosts used incomplete Freund’s. RRID:AB_528211
DSHB Growth Medium :
Iscove's
References (legacy) :
Insect Biochem. Mol. Biol. 25, 845-856.; Dev. Biol. 180, 258-272.
more info or order :
company information
Developmental Studies Hybridoma Bank
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