vimentin (Xenopus) | Developmental Studies Hybridoma Bank 14h7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

vimentin (Xenopus)

catalog :

14h7

clonality :

monoclonal

host :

house mouse

conjugate :

nonconjugated

clone name :

14h7

reactivity :

Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
MARTINEZ DE LUNA R, Ku R, Aruck A, Santiago F, Viczian A, San Mauro D, et al. Müller glia reactivity follows retinal injury despite the absence of the glial fibrillary acidic protein gene in Xenopus. Dev Biol. 2017;426:219-235 pubmed publisher
Dominguez L, Schlosser G, Shen S. Expression of a novel serine/threonine kinase gene, Ulk4, in neural progenitors during Xenopus laevis forebrain development. Neuroscience. 2015;290:61-79 pubmed publisher
Bestman J, Lee Osbourne J, Cline H. In vivo time-lapse imaging of cell proliferation and differentiation in the optic tectum of Xenopus laevis tadpoles. J Comp Neurol. 2012;520:401-33 pubmed publisher
Huang Q, Zhao S, Gaudin A, Quennedey B, Gascuel J. Glial fibrillary acidic protein and vimentin expression in the frog olfactory system during metamorphosis. Neuroreport. 2005;16:1439-42 pubmed
Yoshida M. Intermediate filament proteins define different glial subpopulations. J Neurosci Res. 2001;63:284-9 pubmed
Sakaguchi D, Janick L, Reh T. Basic fibroblast growth factor (FGF-2) induced transdifferentiation of retinal pigment epithelium: generation of retinal neurons and glia. Dev Dyn. 1997;209:387-98 pubmed
Defoe D, Easterling K. Reattachment of retinas to cultured pigment epithelial monolayers from Xenopus laevis. Invest Ophthalmol Vis Sci. 1994;35:2466-76 pubmed
Dent J, Polson A, Klymkowsky M. A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus. Development. 1989;105:61-74 pubmed
Klymkowsky M, Maynell L, Polson A. Polar asymmetry in the organization of the cortical cytokeratin system of Xenopus laevis oocytes and embryos. Development. 1987;100:543-57 pubmed
Dent J, Cary R, Bachant J, Domingo A, Klymkowsky M. Host cell factors controlling vimentin organization in the Xenopus oocyte. J Cell Biol. 1992;119:855-66 pubmed

product information

Internal ID :

2875

Name :

14h7

Depositor Name :

Klymkowsky, M.

Depositor Institution :

Molecular, Cellular & Developmental Biology, University of Colorado

Date Deposited :

11/1/88

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

vimentin (Xenopus)

Antigen Species :

Xenopus

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Xenopus

Species Tested (legacy) :

does not cross react with mouse

Initial Publication Pubmed ID :

2443336

Depositor Notes (Special Instructions) :

This antibody appears to be Xenopus-specific and doesn't cross react with mouse. It recognizes the 55 and 57 kDa vimentin proteins encoded by the genes vim1 and vim4, respectively. 14h7 disrupts vimentin organization when injected into Xenopus A6 cells. 14H7 undergoes frequent class switching from IgG1 to IgG2a.

Collections :

Cytoskeleton

Search Keywords :

Klymkowsky, M., vimentin (Xenopus), , Xenopus, Monoclonal, Xenopus, Western Blot/Immunohistochemistry, function blocking, immunofluorescence, vim1, vim4, AB_528507, Igg1,

Antigen Molecular Weight :

Predicted: 52.8 (vim1) and 53.5 (vim4) kDa; Apparent: 55 (vim1) and 57 (vim4) kDa

Gene :

vim1 & vim4

Uniprot ID :

P24789 P24790

Antibody Registry ID :

AB_528507

Immunogen :

Cell insoluble residues derived from the Xenopus kidney epithelial cell line A6 (mainly containing intermediate filaments)

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

Epitope Mapped :

Recommended Applications :

Function Blocking,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

2806118 7512944

Immunofluorescence Pubmed IDs :

2806118 7512944 1429840 16110267 11170178 9264262 25637795 22113462

Western Blot Pubmed IDs :

2443336 2806118 1429840 26996101

Additional Information :

While this antibody has been reported to be Xenopus-specific, Arciniegas et al. [PMID: 10603448] and Gomes et al. [PMID: 22075471] have reported using it succesfully on chicken embryos and mouse cultured ectoplacental cones, respectively.

DSHB Growth Medium :

RPMI

References (legacy) :

Development 100, 543-557.; Development 105, 61-74.; Invest. Ophthal. & Vis. Sci. 35, 2466-2476.

more info or order :

Developmental Studies Hybridoma Bank product webpage