Robo (Drosophila) | Developmental Studies Hybridoma Bank 13C9 anti-Robo product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Robo (Drosophila)

catalog :

13C9 anti-Robo

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13C9 anti-Robo

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:30; loading ...; fig 4E	Raza Q, Vanderploeg J, Jacobs J. Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development. PLoS ONE. 2017;12:e0171905 pubmed publisher
immunohistochemistry; fruit fly; 1:20; fig 2p	Hanlon C, Andrew D. Drosophila FoxL1 non-autonomously coordinates organ placement during embryonic development. Dev Biol. 2016;419:273-284 pubmed publisher
immunohistochemistry; fruit fly; 1:100	Brown H, Reichert M, Evans T. Slit Binding via the Ig1 Domain Is Essential for Midline Repulsion by Drosophila Robo1 but Dispensable for Receptor Expression, Localization, and Regulation in Vivo. G3 (Bethesda). 2015;5:2429-39 pubmed publisher
western blot; fruit fly; 1:200	Lee J, Peng Y, Lin W, Parrish J. Coordinate control of terminal dendrite patterning and dynamics by the membrane protein Raw. Development. 2015;142:162-73 pubmed publisher
	Slováková J, Speicher S, Sanchez Soriano N, Prokop A, Carmena A. The actin-binding protein Canoe/AF-6 forms a complex with Robo and is required for Slit-Robo signaling during axon pathfinding at the CNS midline. J Neurosci. 2012;32:10035-44 pubmed publisher
	Soplop N, Cheng Y, Kramer S. Roundabout is required in the visceral mesoderm for proper microvillus length in the hindgut epithelium. Dev Dyn. 2012;241:759-69 pubmed publisher
	Smart A, Course M, Rawson J, Selleck S, Van Vactor D, Johnson K. Heparan sulfate proteoglycan specificity during axon pathway formation in the Drosophila embryo. Dev Neurobiol. 2011;71:608-18 pubmed publisher
	Weyers J, Milutinovich A, Takeda Y, Jemc J, Van Doren M. A genetic screen for mutations affecting gonad formation in Drosophila reveals a role for the slit/robo pathway. Dev Biol. 2011;353:217-28 pubmed publisher
	Banerjee S, Blauth K, Peters K, Rogers S, Fanning A, Bhat M. Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance. J Neurosci. 2010;30:5653-67 pubmed publisher
	Katsuki T, Ailani D, Hiramoto M, Hiromi Y. Intra-axonal patterning: intrinsic compartmentalization of the axonal membrane in Drosophila neurons. Neuron. 2009;64:188-99 pubmed publisher
	Berni J, Beckwith E, Fernández M, Ceriani M. The axon-guidance roundabout gene alters the pace of the Drosophila circadian clock. Eur J Neurosci. 2008;27:396-407 pubmed publisher
	Kolesnikov T, Beckendorf S. NETRIN and SLIT guide salivary gland migration. Dev Biol. 2005;284:102-11 pubmed
	Tayler T, Robichaux M, Garrity P. Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins. Development. 2004;131:5935-45 pubmed
	Kraut R, Zinn K. Roundabout 2 regulates migration of sensory neurons by signaling in trans. Curr Biol. 2004;14:1319-29 pubmed
	Kidd T, Brose K, Mitchell K, Fetter R, Tessier Lavigne M, Goodman C, et al. Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors. Cell. 1998;92:205-15 pubmed

product information

Internal ID :

3508

Name :

13C9 anti-Robo

Depositor Name :

Goodman, C.

Depositor Institution :

University of California, Berkeley

Date Deposited :

11/1/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Robo (Drosophila)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

9458045

Depositor Notes (Special Instructions) :

Does not cross-react with Robo2 or Robo3; Use fresh, not MeOH stored, embryo; Works well with with Tween, not Triton.

Collections :

Cell signaling,Drosophila antigens,Neurodevelopment

Search Keywords :

Goodman, Robo, mouse, Slit, neuron midline crossing, AB_2181861, MIgG1, Monoclonal, Drosophila, BEST:GM07780; CG13521; CT32892; D-Robo1; Dmel CG13521; dRobo; dRobo1; Robo; ROBO; Robo-1; robo1; Robo1, Immunofluorescence,Immunohistochemistry,Western Blot

Antigen Molecular Weight :

Predicted: 151.7 kDa; Apparent: 180kDa

Gene :

robo

Alternate Gene Name(s) :

BEST:GM07780; CG13521; CT32892; D-Robo1; Dmel CG13521; dRobo; dRobo1; Robo; ROBO; Robo-1; robo1; Robo1

Uniprot ID :

Q7KVK3

Antibody Registry ID :

AB_2181861

Immunogen :

extracellular portion (amino acids 404_725) of Robo turn from the commissure (Figure 5B) into a longitudinal

Immunogen Sequence :

9458045

Clonality :

Monoclonal

Myeloma Strain :

Epitope Mapped :

Yes

Epitope Location or Sequence :

Extracellular domain aa. 404_725

Epitope Map PubMed ID :

9458045

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

15950216 19874787 15296748 22815517 15525663 21500363

Immunofluorescence Pubmed IDs :

18215236 20410118 22815517 21377458 22334475

Western Blot Pubmed IDs :

20410118 22815517 15525663

Immunoprecipitation Pubmed IDs :

22815517

Depositor Growth Medium :

15% fetal bovine serum in RPMI 1640 with 1x pen/strep

DSHB Growth Medium :

RPMI

References (legacy) :

Cell 92(2), 205-215.

more info or order :

Developmental Studies Hybridoma Bank product webpage