product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
filament antigen
catalog :
12G9
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12G9
application :
western blot, immunohistochemistry
more info or order :
citations: 4
| Reference |
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Strzyzewska Jówko I, Jerka Dziadosz M, Frankel J. Effect of alteration in the global body plan on the deployment of morphogenesis-related protein epitopes labeled by the monoclonal antibody 12G9 in Tetrahymena thermophila. Protist. 2003;154:71-90 pubmed
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Jerka Dziadosz M, Strzyewska Jówko I, Wojsa Lugowska U, Krawczynska W, Krzywicka A. The dynamics of filamentous structures in the apical band, oral crescent, fission line and the postoral meridional filament in Tetrahymena thermophila revealed by monoclonal antibody 12G9. Protist. 2001;152:53-67 pubmed
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Jerka Dziadosz M, Muszyńska K, Krawczynska W. A novel healing filament in ciliate regeneration. J Eukaryot Microbiol. 1999;46:507-15 pubmed
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Jerka Dziadosz M, Jenkins L, Nelsen E, Williams N, Jaeckel Williams R, Frankel J. Cellular polarity in ciliates: persistence of global polarity in a disorganized mutant of Tetrahymena thermophila that disrupts cytoskeletal organization. Dev Biol. 1995;169:644-61 pubmed
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product information
Internal ID :
3190
Name :
12G9
Depositor Name :
Frankel, J./Nelsen, E.M./Jerka-Dziadosz, M.
Depositor Institution :
Cell Biology M. Nencki Institute of Experimental Biology
Date Deposited :
10/6/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
filament antigen
Antigen Species :
Tetrahymena thermophila
Host Species :
mouse
Isotype :
MIgM
Isotype for catalog (legacy) :
IgM
Positive Tested Species Reactivity :
Protozoa,Tetrahymena,Vertebrate
Species Tested (legacy) :
ciliates, some vertebrate cells, not Saccharomyces cerevisiae
Initial Publication Pubmed ID :
7781905
Collections :
Cell adhesion,Cytoskeleton,Microbe antibodies
Search Keywords :
Frankel, J./Nelsen, E.M./Jerka-Dziadosz, M., filament antigen, MIgM, Tetrahymena/Protozoa/vertebrate, Monoclonal, Tetrahymena thermophila, Immunohistochemistry/Western Blot
Antigen Molecular Weight :
140 kDa cortical protein in T. thermophila
Antibody Registry ID :
AB_531773
Immunogen :
Tetrahymena thermophila (a ciliated protozoan)
Clonality :
Monoclonal
Myeloma Strain :
Designation of NS-1
Epitope Mapped :
No
Recommended Applications :
Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Pubmed IDs :
7781905 10519219 11401037 12812371
Additional Information :
The origin of the antibody is deciliated plasma membrane & surface cytoskeleton of Tetrahymena thermophila, isolated by procedure of Nozawa & Thompson (J. Cell Biol. 49, 712-721). RRID:AB_531773
DSHB Growth Medium :
Iscove's
References (legacy) :
Dev. Biol. 169, 644-661.; Protoplasma 197, 241-257.; J. Euk. Microbiol. 46, 507-515.; Protist 152, 53-67.; Protist 154, 71-90.
more info or order :
company information
Developmental Studies Hybridoma Bank
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