product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
tubulin (alpha-)
catalog :
12G10 anti-alpha-tubulin
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12G10 anti-alpha-tubulin
reactivity :
Anomura, Pagurus, Dictyostelium discoideum, American cockroach, Neogonodactylus oerstedii, human, mouse, Drosophila melanogaster, Tetrahymena thermophila SB210
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 41
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Pagurus; 1:100; loading ...; fig 1b
Strausfeld N, Sayre M. Mushroom bodies in Reptantia reflect a major transition in crustacean brain evolution. J Comp Neurol. 2020;528:261-282 pubmed publisher
  • immunohistochemistry; Neogonodactylus oerstedii; 1:100; loading ...; fig 2a
Thoen H, Wolff G, Marshall J, Sayre M, Strausfeld N. The reniform body: An integrative lateral protocerebral neuropil complex of Eumalacostraca identified in Stomatopoda and Brachyura. J Comp Neurol. 2019;: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2c
Hariri B, McMahon D, Chen B, Freund J, Mansfield C, Doghramji L, et al. Flavones modulate respiratory epithelial innate immunity: Anti-inflammatory effects and activation of the T2R14 receptor. J Biol Chem. 2017;292:8484-8497 pubmed publisher
  • western blot; human; 1:5000; fig 6a
Kaukonen R, Mai A, Georgiadou M, Saari M, De Franceschi N, Betz T, et al. Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription. Nat Commun. 2016;7:12237 pubmed publisher
  • western blot; human; 1:2000; fig 3
Ogaki S, Morooka M, Otera K, Kume S. A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells. Sci Rep. 2015;5:17297 pubmed publisher
  • western blot; Dictyostelium discoideum; 1:1000; fig 4f
Santarriaga S, Petersen A, Ndukwe K, Brandt A, GERGES N, Bruns Scaglione J, et al. The Social Amoeba Dictyostelium discoideum Is Highly Resistant to Polyglutamine Aggregation. J Biol Chem. 2015;290:25571-8 pubmed publisher
  • western blot; mouse; 1:1000
Sun D, Buttitta L. Protein phosphatase 2A promotes the transition to G0 during terminal differentiation in Drosophila. Development. 2015;142:3033-45 pubmed publisher
  • western blot; Drosophila melanogaster; 1:2000
Lee J, Peng Y, Lin W, Parrish J. Coordinate control of terminal dendrite patterning and dynamics by the membrane protein Raw. Development. 2015;142:162-73 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100
  • western blot; mouse
Siggers P, Carré G, Bogani D, Warr N, Wells S, Hilton H, et al. A novel mouse Fgfr2 mutant, hobbyhorse (hob), exhibits complete XY gonadal sex reversal. PLoS ONE. 2014;9:e100447 pubmed publisher
  • western blot; human
Hauerslev S, Sveen M, Vissing J, Krag T. Protein turnover and cellular stress in mildly and severely affected muscles from patients with limb girdle muscular dystrophy type 2I. PLoS ONE. 2013;8:e66929 pubmed publisher
  • western blot; human
Pe er T, Lahmi R, Sharaby Y, Chorni E, Noach M, Vecsler M, et al. Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex. PLoS ONE. 2013;8:e57532 pubmed publisher
  • immunohistochemistry - paraffin section; American cockroach
  • immunohistochemistry - paraffin section; Anomura
Wolff G, Harzsch S, Hansson B, Brown S, STRAUSFELD N. Neuronal organization of the hemiellipsoid body of the land hermit crab, Coenobita clypeatus: correspondence with the mushroom body ground pattern. J Comp Neurol. 2012;520:2824-46 pubmed publisher
  • western blot; Drosophila melanogaster; 1:10
Koppen T, Weckmann A, Muller S, Staubach S, Bloch W, Dohmen R, et al. Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. Proteomics. 2011;11:4397-410 pubmed publisher
Yordanov T, Hipolito V, Liebscher G, Vogel G, Stasyk T, Herrmann C, et al. Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate. Traffic. 2019;20:674-696 pubmed publisher
Junker A, Soh A, O TOOLE E, Meehl J, Guha M, Winey M, et al. Microtubule glycylation promotes attachment of basal bodies to the cell cortex. J Cell Sci. 2019;132: pubmed publisher
Wan J, Block S, Scribano C, Thiry R, Esbona K, Audhya A, et al. Mad1 destabilizes p53 by preventing PML from sequestering MDM2. Nat Commun. 2019;10:1540 pubmed publisher
Dhanda A, Lulic K, Vogl A, Mc Gee M, CHIU R, Guttman J. Listeria Membrane Protrusion Collapse: Requirement of Cyclophilin A for Listeria Cell-to-Cell Spreading. J Infect Dis. 2019;219:145-153 pubmed publisher
Ogunsakin O, Sriyotha P, Burns T, Hottor T, McCaskill M. Diallyl Disulfide inhibits ethanol-induced pulmonary cell vitamin D and antimicrobial peptide Cathelicidin depletion. Alcohol. 2018;: pubmed publisher
Haque M, Mohibbullah M, Hong Y, Moon I. Calotropis gigantea Promotes Neuritogenesis and Synaptogenesis through Activation of NGF-TrkA-Erk1/2 Signaling in Rat Hippocampal Neurons. Am J Chin Med. 2018;46:1861-1877 pubmed publisher
Noree C. Extramitochondrial Assembly of Mitochondrial Targeting Signal Disrupted Mitochondrial Enzyme Aldehyde Dehydrogenase. Sci Rep. 2018;8:6186 pubmed publisher
Westlake H, Page L. Muscle and nerve net organization in stalked jellyfish (Medusozoa: Staurozoa). J Morphol. 2017;278:29-49 pubmed publisher
Koizumi M, Shigeno S, Mizunami M, Tanaka N. Three-dimensional brain atlas of pygmy squid, Idiosepius paradoxus, revealing the largest relative vertical lobe system volume among the cephalopods. J Comp Neurol. 2016;524:2142-57 pubmed publisher
Benbahouche N, Iliopoulos I, Török I, Marhold J, Henri J, Kajava A, et al. Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries. J Biol Chem. 2014;289:6236-47 pubmed publisher
Krag T, Hauerslev S, Dahlqvist J, Vissing J. Muscle biopsies off-set normal cellular signaling in surrounding musculature. Neuromuscul Disord. 2013;23:981-5 pubmed publisher
Alaeddine F, Hemphill A, Debache K, Guionaud C. Molecular cloning and characterization of NcROP2Fam-1, a member of the ROP2 family of rhoptry proteins in Neospora caninum that is targeted by antibodies neutralizing host cell invasion in vitro. Parasitology. 2013;140:1033-50 pubmed publisher
Fukui A, Inaki M, Tonoe G, Hamatani H, Homma M, Morimoto T, et al. Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction. Biol Open. 2012;1:362-75 pubmed publisher
Dickinson D, Robinson D, Nelson W, Weis W. ?-catenin and IQGAP regulate myosin localization to control epithelial tube morphogenesis in Dictyostelium. Dev Cell. 2012;23:533-46 pubmed publisher
Hannan M, Kang J, Hong Y, Lee H, Chowdhury M, Choi J, et al. A brown alga Sargassum fulvellum facilitates neuronal maturation and synaptogenesis. In Vitro Cell Dev Biol Anim. 2012;48:535-44 pubmed publisher
Brown S, Wolff G. Fine structural organization of the hemiellipsoid body of the land hermit crab, Coenobita clypeatus. J Comp Neurol. 2012;520:2847-63 pubmed publisher
Ritter L, Khattree N, Tam B, Moritz O, Schmitz F, Goldberg A. In situ visualization of protein interactions in sensory neurons: glutamic acid-rich proteins (GARPs) play differential roles for photoreceptor outer segment scaffolding. J Neurosci. 2011;31:11231-43 pubmed publisher
Drévillon L, Tanguy G, Hinzpeter A, Arous N, de Becdelievre A, Aissat A, et al. COMMD1-mediated ubiquitination regulates CFTR trafficking. PLoS ONE. 2011;6:e18334 pubmed publisher
Rogowski K, Van Dijk J, Magiera M, Bosc C, Deloulme J, Bosson A, et al. A family of protein-deglutamylating enzymes associated with neurodegeneration. Cell. 2010;143:564-78 pubmed publisher
Suryavanshi S, Edde B, Fox L, Guerrero S, Hard R, Hennessey T, et al. Tubulin glutamylation regulates ciliary motility by altering inner dynein arm activity. Curr Biol. 2010;20:435-40 pubmed publisher
Kanfi Y, Peshti V, Gil R, Naiman S, Nahum L, Levin E, et al. SIRT6 protects against pathological damage caused by diet-induced obesity. Aging Cell. 2010;9:162-73 pubmed publisher
Wloga D, Dave D, Meagley J, Rogowski K, Jerka Dziadosz M, Gaertig J. Hyperglutamylation of tubulin can either stabilize or destabilize microtubules in the same cell. Eukaryot Cell. 2010;9:184-93 pubmed publisher
Brauchle M, Kiontke K, MacMenamin P, Fitch D, Piano F. Evolution of early embryogenesis in rhabditid nematodes. Dev Biol. 2009;335:253-62 pubmed publisher
Rogowski K, Juge F, Van Dijk J, Wloga D, Strub J, Levilliers N, et al. Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation. Cell. 2009;137:1076-87 pubmed publisher
Mohammad M, Raftos D, Joss J. Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri. J Anat. 2009;214:140-52 pubmed publisher
Thazhath R, Liu C, Gaertig J. Polyglycylation domain of beta-tubulin maintains axonemal architecture and affects cytokinesis in Tetrahymena. Nat Cell Biol. 2002;4:256-9 pubmed
Breaz P, Dumitrascu D, Grigorescu M, Pascu O, Porr P, Goia A, et al. [Digestive tract involvement in collagenoses]. Rev Med Interna Neurol Psihiatr Neurochir Dermatovenerol Med Interna. 1987;39:513-20 pubmed
Alcalay J, David M. [Darker side of tanning from artificial radiation source]. Harefuah. 1989;117:273-4 pubmed
product information
Internal ID :
3184
Name :
12G10 anti-alpha-tubulin
Depositor Name :
Frankel, J. / Nelsen, E.M.
Depositor Institution :
University of Iowa
Date Deposited :
9/21/01
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
tubulin (alpha-)
Antigen Species :
Tetrahymena
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Algae,C. elegans,Cephalopod,Dictyostelium,Drosophila,Fish,Hermit Crab,Human,Medusa,Mouse,Protozoa,Saccharomyces,Tetrahymena,Xenopus
Species Tested (legacy) :
multiple species (Tetrhymena, Dictyostelium, human, Saccharomyces)
Initial Publication Pubmed ID :
11862218
Collections :
Cytoskeleton,Drosophila antigens,Human,Microbe antibodies,Neurodevelopment,Xenopus
Search Keywords :
Frankel, Nelsen, tubulin (alpha-), mouse, AB_1157911, MIgG1, Monoclonal, Tetrahymena, Dictyostelium, Human, Saccharomyces, Xenopus, Drosophila, Protozoa, Algae, Fish, C. elegans, Mouse, Hermit crab
Antigen Molecular Weight :
Predicted: 49.59 kDa
Gene :
ATU1
Uniprot ID :
P41351
Antibody Registry ID :
AB_1157911
Immunogen :
alpha tubulin, Tetrahymena thermophila and Tetrahymena pyriformis (mixture)
Immunogen Sequence :
alpha tubulin
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
No
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
20189389 19700636 23743240 22893212 21074048
Immunofluorescence Pubmed IDs :
19700636 2895946 23743240 21074048 19643102 22902739 27696494 26663197 26663197 30518234 31243050
Western Blot Pubmed IDs :
24148530 2613100 21813684 29670139 21483833 23213426 20047575 24394412 19524510 30580017 30948704 31314175 29733369
FFPE Pubmed IDs :
22318704 19166477 20047575
Depositor Growth Medium :
20% fetal bovine serum in Iscove's DMEM with 1x pen/strep
DSHB Growth Medium :
Iscove's
References (legacy) :
Nature Cell Biol. 4, 256-259.; Dev. Biol. 335, 253-262.; Dev. Cell 23, 533-546.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US