versican (hyaluronate-binding region) | Developmental Studies Hybridoma Bank 12C5 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

versican (hyaluronate-binding region)

catalog :

12C5

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12C5

reactivity :

giant panda, human, rat, dog, cow, pig

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 46

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; scFv; 1:2000; fig 2c	Sari A, Rufaut N, Jones L, Sinclair R. Characterization of Ovine Dermal Papilla Cell Aggregation. Int J Trichology. 2016;8:121-9 pubmed publisher
immunocytochemistry; human; 1:2000; fig 1f	Sari A, Rufaut N, Jones L, Sinclair R. The Effect of Ovine Secreted Soluble Factors on Human Dermal Papilla Cell Aggregation. Int J Trichology. 2016;8:103-10 pubmed publisher
immunohistochemistry - paraffin section; pig; 1:100; fig 4	Rai V, Dietz N, Dilisio M, Radwan M, Agrawal D. Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine. Arthritis Res Ther. 2016;18:203 pubmed publisher
western blot; human; fig 5	Yang Y, Sun Y, Acott T, Keller K. Effects of induction and inhibition of matrix cross-linking on remodeling of the aqueous outflow resistance by ocular trabecular meshwork cells. Sci Rep. 2016;6:30505 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:100; loading ...; tbl 1	RÃ¡cz Ã, GaÃ¡l B, Matesz C. Heterogeneous expression of extracellular matrix molecules in the red nucleus of the rat. Neuroscience. 2016;322:1-17 pubmed publisher
immunohistochemistry; giant panda; 1:10; fig 1	Prescott H, Manning C, Gardner A, Ritchie W, Pizzi R, Girling S, et al. Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells. PLoS ONE. 2015;10:e0138840 pubmed publisher
immunohistochemistry; rat; 1:100; fig 1	Kecskes S, GaÃ¡l B, RÃ¡cz Ã, Birinyi A, Hunyadi A, Matesz C. Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive. Neuroscience. 2015;284:412-21 pubmed publisher
immunocytochemistry; human; 1:5	Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed
	Damasceno K, Ferreira E, Estrela Lima A, Gamba C, Miranda F, Alves M, et al. HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland. PLoS ONE. 2016;11:e0160419 pubmed publisher
	Bukong T, Maurice S, Chahal B, Schaeffer D, Winwood P. Versican: a novel modulator of hepatic fibrosis. Lab Invest. 2016;96:361-74 pubmed publisher
	Desjardins M, Xie J, Gurler H, Muralidhar G, Sacks J, Burdette J, et al. Versican regulates metastasis of epithelial ovarian carcinoma cells and spheroids. J Ovarian Res. 2014;7:70 pubmed publisher
	Damasceno K, Bertagnolli A, Estrela Lima A, Rabelo B, Campos L, Ribeiro L, et al. Versican expression in myoepithelial cells from carcinomas in canine mixed mammary tumors. Vet J. 2014;200:146-51 pubmed publisher
	Ming L, Wilk R, Reed B, Lipshitz H. Drosophila Hindsight and mammalian RREB-1 are evolutionarily conserved DNA-binding transcriptional attenuators. Differentiation. 2013;86:159-70 pubmed publisher
	Hummitzsch K, Irving Rodgers H, Hatzirodos N, Bonner W, Sabatier L, Reinhardt D, et al. A new model of development of the mammalian ovary and follicles. PLoS ONE. 2013;8:e55578 pubmed publisher
	Keenan T, Clark S, Unwin R, Ridge L, Day A, Bishop P. Mapping the differential distribution of proteoglycan core proteins in the adult human retina, choroid, and sclera. Invest Ophthalmol Vis Sci. 2012;53:7528-38 pubmed publisher
	Kawase T, Shibata S, Katori Y, Ohtsuka A, Murakami G, Fujimiya M. Elastic fiber-mediated enthesis in the human middle ear. J Anat. 2012;221:331-40 pubmed publisher
	Said N, Sanchez Carbayo M, Smith S, Theodorescu D. RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. 2012;122:1503-18 pubmed publisher
	Hatzirodos N, Nigro J, Irving Rodgers H, Vashi A, Hummitzsch K, Caterson B, et al. Glycomic analyses of ovarian follicles during development and atresia. Matrix Biol. 2012;31:45-56 pubmed publisher
	Kischel P, Waltregny D, Greffe Y, Mazzucchelli G, De Pauw E, de Leval L, et al. Identification of stromal proteins overexpressed in nodular sclerosis Hodgkin lymphoma. Proteome Sci. 2011;9:63 pubmed publisher
	Kharaishvili G, Cizkova M, Bouchalova K, Mgebrishvili G, Kolar Z, Bouchal J. Collagen triple helix repeat containing 1 protein, periostin and versican in primary and metastatic breast cancer: an immunohistochemical study. J Clin Pathol. 2011;64:977-82 pubmed publisher
	Carrino D, Calabro A, Darr A, Dours Zimmermann M, Sandy J, Zimmermann D, et al. Age-related differences in human skin proteoglycans. Glycobiology. 2011;21:257-68 pubmed publisher
	Potter Perigo S, Johnson P, Evanko S, Chan C, Braun K, Wilkinson T, et al. Polyinosine-polycytidylic acid stimulates versican accumulation in the extracellular matrix promoting monocyte adhesion. Am J Respir Cell Mol Biol. 2010;43:109-20 pubmed publisher
	Bogen O, Joseph E, Chen X, Levine J. GDNF hyperalgesia is mediated by PLCgamma, MAPK/ERK, PI3K, CDK5 and Src family kinase signaling and dependent on the IB4-binding protein versican. Eur J Neurosci. 2008;28:12-9 pubmed publisher
	Melrose J, Smith S, Appleyard R, Little C. Aggrecan, versican and type VI collagen are components of annular translamellar crossbridges in the intervertebral disc. Eur Spine J. 2008;17:314-24 pubmed publisher
	Castronovo V, Kischel P, Guillonneau F, de Leval L, Defechereux T, De Pauw E, et al. Identification of specific reachable molecular targets in human breast cancer using a versatile ex vivo proteomic method. Proteomics. 2007;7:1188-96 pubmed
	Vitellaro Zuccarello L, Mazzetti S, Madaschi L, Bosisio P, Gorio A, De Biasi S. Erythropoietin-mediated preservation of the white matter in rat spinal cord injury. Neuroscience. 2007;144:865-77 pubmed
	Le Bellego F, Plante S, Chakir J, Hamid Q, Ludwig M. Differences in MAP kinase phosphorylation in response to mechanical strain in asthmatic fibroblasts. Respir Res. 2006;7:68 pubmed
	Lensen J, Rops A, Wijnhoven T, Hafmans T, Feitz W, Oosterwijk E, et al. Localization and functional characterization of glycosaminoglycan domains in the normal human kidney as revealed by phage display-derived single chain antibodies. J Am Soc Nephrol. 2005;16:1279-88 pubmed
	Bogen O, Dreger M, Gillen C, Schroder W, Hucho F. Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal cord tissue. FEBS J. 2005;272:1090-102 pubmed
	Young H. Existence of reserve quiescent stem cells in adults, from amphibians to humans. Curr Top Microbiol Immunol. 2004;280:71-109 pubmed
	Shi S, Ciurli C, Cartman A, Pidoux I, Poole A, Zhang Y. Experimental immunity to the G1 domain of the proteoglycan versican induces spondylitis and sacroiliitis, of a kind seen in human spondylarthropathies. Arthritis Rheum. 2003;48:2903-15 pubmed
	Tang X, Davies J, Davies S. Changes in distribution, cell associations, and protein expression levels of NG2, neurocan, phosphacan, brevican, versican V2, and tenascin-C during acute to chronic maturation of spinal cord scar tissue. J Neurosci Res. 2003;71:427-44 pubmed
	Cattaruzza S, Schiappacassi M, Ljungberg Rose A, Spessotto P, Perissinotto D, Morgelin M, et al. Distribution of PG-M/versican variants in human tissues and de novo expression of isoform V3 upon endothelial cell activation, migration, and neoangiogenesis in vitro. J Biol Chem. 2002;277:47626-35 pubmed
	Tischer T, Milz S, Maier M, Schieker M, Benjamin M. An immunohistochemical study of the rabbit suprapatella, a sesamoid fibrocartilage in the quadriceps tendon containing aggrecan. J Histochem Cytochem. 2002;50:955-60 pubmed
	Asher R, Morgenstern D, Shearer M, Adcock K, Pesheva P, Fawcett J. Versican is upregulated in CNS injury and is a product of oligodendrocyte lineage cells. J Neurosci. 2002;22:2225-36 pubmed
	Milz S, Schlüter T, Putz R, Moriggl B, Ralphs J, Benjamin M. Fibrocartilage in the transverse ligament of the human atlas. Spine (Phila Pa 1976). 2001;26:1765-71 pubmed
	Boszczyk B, Boszczyk A, Putz R, Büttner A, Benjamin M, Milz S. An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints. Spine (Phila Pa 1976). 2001;26:E338-43 pubmed
	Milz S, Valassis G, Büttner A, Maier M, Putz R, Ralphs J, et al. Fibrocartilage in the transverse ligament of the human acetabulum. J Anat. 2001;198:223-8 pubmed
	Fidler P, Schuette K, Asher R, Dobbertin A, Thornton S, Calle Patino Y, et al. Comparing astrocytic cell lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan is NG2. J Neurosci. 1999;19:8778-88 pubmed
	Reardon A, Heinegard D, McLeod D, Sheehan J, Bishop P. The large chondroitin sulphate proteoglycan versican in mammalian vitreous. Matrix Biol. 1998;17:325-33 pubmed
	Perides G, Asher R, Lark M, Lane W, Robinson R, Bignami A. Glial hyaluronate-binding protein: a product of metalloproteinase digestion of versican?. Biochem J. 1995;312 ( Pt 2):377-84 pubmed
	Asher R, Scheibe R, Keiser H, Bignami A. On the existence of a cartilage-like proteoglycan and link proteins in the central nervous system. Glia. 1995;13:294-308 pubmed
	Gauthier G. Differential distribution of myosin isoforms among the myofibrils of individual developing muscle fibers. J Cell Biol. 1990;110:693-701 pubmed
	Kwok D, Pillai G, Vaughan R, Axelson J, McErlane K. Preparative high-performance liquid chromatography and preparative thin-layer chromatography isolation of tocainide carbamoyl-O-beta-D-glucuronide: structural characterization by gas chromatography-mass spectrometry and fast atom bombardment-mass spe. J Pharm Sci. 1990;79:857-61 pubmed
	Asher R, Perides G, Vanderhaeghen J, Bignami A. Extracellular matrix of central nervous system white matter: demonstration of an hyaluronate-protein complex. J Neurosci Res. 1991;28:410-21 pubmed
	Perides G, Rahemtulla F, Lane W, Asher R, Bignami A. Isolation of a large aggregating proteoglycan from human brain. J Biol Chem. 1992;267:23883-7 pubmed

product information

Internal ID :

451

Name :

12C5

Depositor Name :

Asher, R.A.

Depositor Institution :

Cambridge Centre for Brain Repair

Date Deposited :

1/18/95

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

versican (hyaluronate-binding region)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,Canine,Human,Porcine,Rat

Species Tested (legacy) :

human, bovine, porcine, canine, rat

Initial Publication Pubmed ID :

1713274

Depositor Notes (Special Instructions) :

Blot: yes, but sensitive to reduction

Collections :

Cardiac development,Cell adhesion,Extracellular matrix proteins,Human,Neurodevelopment

Search Keywords :

Asher, versican (hyaluronate-binding region), AB_528503, MIgG1, Monoclonal, Human, Bovine, Porcine, Canine, Rat, VCAN, WGN; ERVR; GHAP; PG-M; WGN1; CSPG2, ELISA,FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Microarray,Western Blot

Antigen Molecular Weight :

Apparent: >250kD

Gene :

VCAN

Alternate Gene Name(s) :

WGN; ERVR; GHAP; PG-M; WGN1; CSPG2

Uniprot ID :

P13611

Antibody Registry ID :

AB_528503

Immunogen :

hyaluronate-binding region of versican (aka _GHAP_)

Immunogen Sequence :

same as antigen sequence.

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

G1 domain

Epitope Map PubMed ID :

12221092

Recommended Applications :

ELISA,FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Microarray,Western Blot

Immunoblotting (legacy) :

yes, sensitive to reduction

Immunohistochemistry Pubmed IDs :

1713274 11896162 12526031 2280351 12221092 17141961 10516297 19717812 21975223 15788473 22803514 11474365 11493848 11273046 14594208

Immunofluorescence Pubmed IDs :

22803514 2307704 23074202 1429726 12526031 11896162 24999371 7615338

Western Blot Pubmed IDs :

1713274 11896162 12526031 12221092 10516297 20947661 19717812 22406535 24999371 7615338

Immunoprecipitation Pubmed IDs :

8526845 10516297

ELISA Pubmed IDs :

10516297 9822199 14558097

FFPE Pubmed IDs :

21975223 17972112 12070274 14558097 24582132

Function Blocking Pubmed IDs :

24999371

Microarray Pubmed IDs :

17372937

Pubmed IDs :

24418439

Depositor Growth Medium :

20% fetal bovine serum in Iscove's DMEM with 1x pen/strep

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Neurosci. Res. 28, 410-421.; Glia 13, 294-308.; Spine 26(15), E338-E343.; Spine 26(16), 1765-1771.; J. Anat. 198, 223-228.; Invest. Ophthalmol. Vis. Sci. 43(5), 1330-1334.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.; FEBS J. 272, 1090-1102.; Eur. J. Neurosci. 28, 12-19.; J. Clin. Pathol. 64, 977-982.

more info or order :

Developmental Studies Hybridoma Bank product webpage