Frizzled2 | Developmental Studies Hybridoma Bank 12A7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Frizzled2

catalog :

12A7

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12A7

reactivity :

, human, Drosophila melanogaster

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; Drosophila melanogaster; 1:20	Liu M, Li Y, Liu A, Li R, Su Y, Du J, et al. The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development. elife. 2016;5: pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:20; fig 2	Agrawal T, Hasan G. Maturation of a central brain flight circuit in Drosophila requires Fz2/Ca²⁺ signaling. elife. 2015;4: pubmed publisher
	Arii J, Watanabe M, Maeda F, Tokai Nishizumi N, Chihara T, Miura M, et al. ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity. Nat Commun. 2018;9:3379 pubmed publisher
	Muller H, Samanta R, Wieschaus E. Wingless signaling in the Drosophila embryo: zygotic requirements and the role of the frizzled genes. Development. 1999;126:577-86 pubmed

product information

Internal ID :

3915

Name :

12A7

Depositor Name :

Nusse, R.

Depositor Institution :

Department of Developmental Biology/HHMI

Date Deposited :

12/10/04

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Frizzled2

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG2a, lambda light chain

Isotype for catalog (legacy) :

IgG2a, lambda light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

9876186

Collections :

Cell signaling,Drosophila antigens

Search Keywords :

Nusse, R., Frizzled2, MIgG2a, lambda light chain, Drosophila, Monoclonal, Drosophila, Western blot/Immunohistochemistry

Antibody Registry ID :

AB_528257

Immunogen :

a fusion protein expressed in E. coli

Clonality :

Monoclonal

Myeloma Strain :

Designation of SP2/0

Epitope Mapped :

Recommended Applications :

Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Pubmed IDs :

9876186

Additional Information :

The origin of the antibody is the cytoplasmic tail of fz2; the last 89 amino acids were fused to GST. Immunization used 30 µg/boost in 1997/1998. RRID:AB_528257

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 126, 577-586.

more info or order :

Developmental Studies Hybridoma Bank product webpage