Aggrecan core protein | Developmental Studies Hybridoma Bank 12/21/1-C-6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Aggrecan core protein

catalog :

12/21/1-C-6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12/21/1-C-6

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:100; fig 3a	Whitaker A, Berthet E, Cantu A, Laird D, Alliston T. Smad4 regulates growth plate matrix production and chondrocyte polarity. Biol Open. 2017;6:358-364 pubmed publisher
	Ouyang A, Cerchiari A, Tang X, Liebenberg E, Alliston T, Gartner Z, et al. Effects of cell type and configuration on anabolic and catabolic activity in 3D co-culture of mesenchymal stem cells and nucleus pulposus cells. J Orthop Res. 2017;35:61-73 pubmed publisher
	Beckmann R, Lippross S, Hartz C, Tohidnezhad M, Ferreira M, Neuss Stein S, et al. Abrasion arthroplasty increases mesenchymal stem cell content of postoperative joint effusions. BMC Musculoskelet Disord. 2015;16:250 pubmed publisher
	Liu J, Li Q, Kuehn M, Litingtung Y, Vokes S, Chiang C. Sonic hedgehog signaling directly targets Hyaluronic Acid Synthase 2, an essential regulator of phalangeal joint patterning. Dev Biol. 2013;375:160-71 pubmed publisher
	Young H, Black A. Adult stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2004;276:75-102 pubmed
	Young H. Existence of reserve quiescent stem cells in adults, from amphibians to humans. Curr Top Microbiol Immunol. 2004;280:71-109 pubmed
	Milz S, Regner F, Putz R, Benjamin M. Expression of a wide range of extracellular matrix molecules in the tendon and trochlea of the human superior oblique muscle. Invest Ophthalmol Vis Sci. 2002;43:1330-4 pubmed
	Milz S, Schlüter T, Putz R, Moriggl B, Ralphs J, Benjamin M. Fibrocartilage in the transverse ligament of the human atlas. Spine (Phila Pa 1976). 2001;26:1765-71 pubmed
	Boszczyk B, Boszczyk A, Putz R, Büttner A, Benjamin M, Milz S. An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints. Spine (Phila Pa 1976). 2001;26:E338-43 pubmed
	Milz S, Valassis G, Büttner A, Maier M, Putz R, Ralphs J, et al. Fibrocartilage in the transverse ligament of the human acetabulum. J Anat. 2001;198:223-8 pubmed
	Milz S, Putz R, Ralphs J, Benjamin M. Fibrocartilage in the extensor tendons of the human metacarpophalangeal joints. Anat Rec. 1999;256:139-45 pubmed
	Calabro A, Hascall V, Caterson B. Monoclonal antibodies directed against epitopes within the core protein structure of the large aggregating proteoglycan (aggrecan) from the swarm rat chondrosarcoma. Arch Biochem Biophys. 1992;298:349-60 pubmed

product information

Internal ID :

1724

Name :

12/21/1-C-6

Depositor Name :

Caterson, B.

Depositor Institution :

Nat. Institute of Dental Research, NIH/ Cardiff University

Date Deposited :

6/1/86

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Aggrecan core protein

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,Canine,Chicken,Fish,Human,Pig,Rat

Species Tested (legacy) :

cross-reacts with hyaluronic acid binding proteoglycan of rat, human, dog, bovine, pig, chicken and hagfish cartilage and non-cartilage tissue (e.g. muscle and aorta)

Initial Publication Pubmed ID :

1384430

Depositor Notes (Special Instructions) :

To expose the core protein epitope, the tissue must be reduced and alkylated. Treat the tissue with 10mM dithiothreitol in TBS pH 7.4 for 2 hr at 37ºC for 2 hr. Follow by alkalation with 40 mM iodoacetamide in PBS for 2 hr.

Collections :

Extracellular matrix proteins,Human

Search Keywords :

Caterson, Aggrecan core protein, Rat , MIgG1, Bovine/Canine/Chicken/Fish/Human/Pig/Rat, ACAN, AGC1, CSPG1, CSPGCP, MSK16, AB_531912, Monoclonal, Human/Extracellular Matrix proteins, Immunohistochemistry/Immunofluorescence/FFPE

Antigen Molecular Weight :

221.9kDa

Gene :

ACAN

Alternate Gene Name(s) :

AGC1, CSPG1, CSPGCP, MSK16

Uniprot ID :

Q61282

Antibody Registry ID :

AB_531912

Immunogen :

Reduced and alkylated G3 domain at the C-terminus

Immunogen Sequence :

Protein domain

Clonality :

Monoclonal

Myeloma Strain :

X63-Ag8.653

Epitope Mapped :

Recommended Applications :

FFPE,Immunofluorescence,Immunohistochemistry

Immunohistochemistry Pubmed IDs :

28167493 27699833 27340938 28919847 25736479 25497864 24320935 24441192 23313125 22674505 21086000 19537875 18539921 18399959 14699636 14594208 11980843 11493848 11474365 11273046 10486511 1384430 26364138

Immunofluorescence Pubmed IDs :

16115612 14983513

FFPE Pubmed IDs :

22674505 23313125 19537875 18539921 26364138

FACS Pubmed IDs :

26364138

Pubmed IDs :

10486511 11474365 11493848 11273046 11980843 14594208 14699636 23313125

Additional Information :

This antibody has been used successfully on native dot blots (PMID:7632088). RRID: AB_531912

DSHB Growth Medium :

RPMI

References (legacy) :

In: Articular Cartilage Biochemistry (K. Kuettner et al., editors), pp.59-73, Raven Press, New York.; Anat. Rec. 256, 139-145.; Spine 26(15), E338-E343.; Spine 26(16), 1765-1771.; J. Anat. 198, 223-228.; Invest. Ophthalmol. Vis. Sci. 43(5), 1330-1334.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.; Dev. Biol. 375, 160-171.

more info or order :

Developmental Studies Hybridoma Bank product webpage