product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
myosin heavy chain, fast, 2B
catalog :
10F5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10F5
reactivity :
mouse, rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 29
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:50; loading ...; fig 1d
  • western blot; mouse; 1:500; loading ...; fig 1b
Zhang Y, Li W, Zhu M, Li Y, Xu Z, Zuo B. FHL3 differentially regulates the expression of MyHC isoforms through interactions with MyoD and pCREB. Cell Signal. 2016;28:60-73 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:600; fig 9
Seaberg B, Henslee G, Wang S, Paez Colasante X, Landreth G, Rimer M. Muscle-derived extracellular signal-regulated kinases 1 and 2 are required for the maintenance of adult myofibers and their neuromuscular junctions. Mol Cell Biol. 2015;35:1238-53 pubmed publisher
Thomas M, Wang D, D Souza D, Krause M, Layne A, Criswell D, et al. Muscle-specific AMPK ?1?2-null mice display a myopathy due to loss of capillary density in nonpostural muscles. FASEB J. 2014;28:2098-107 pubmed publisher
Kim G, Ryu Y, Jeong J, Yang H, Joo S. Relationship between pork quality and characteristics of muscle fibers classified by the distribution of myosin heavy chain isoforms. J Anim Sci. 2013;91:5525-34 pubmed publisher
Sitnick M, Basantani M, Cai L, Schoiswohl G, Yazbeck C, Distefano G, et al. Skeletal muscle triacylglycerol hydrolysis does not influence metabolic complications of obesity. Diabetes. 2013;62:3350-61 pubmed publisher
Lee N, Spearry R, Leahy K, Robitz R, Trinh D, Mason C, et al. Muscle ciliary neurotrophic factor receptor α promotes axonal regeneration and functional recovery following peripheral nerve lesion. J Comp Neurol. 2013;521:2947-65 pubmed publisher
Kim G, Jeong J, Yang H, Joo S. Identification of myosin heavy chain isoforms in porcine longissimus dorsi muscle by electrophoresis and mass spectrometry. Electrophoresis. 2013;34:1255-61 pubmed publisher
Begue G, Douillard A, Galbes O, Rossano B, Vernus B, Candau R, et al. Early activation of rat skeletal muscle IL-6/STAT1/STAT3 dependent gene expression in resistance exercise linked to hypertrophy. PLoS ONE. 2013;8:e57141 pubmed publisher
Heller K, Montgomery C, Janssen P, Clark K, Mendell J, Rodino Klapac L. AAV-mediated overexpression of human ?7 integrin leads to histological and functional improvement in dystrophic mice. Mol Ther. 2013;21:520-5 pubmed publisher
Hazimihalis P, Gorvet M, Butcher M. Myosin isoform fiber type and fiber size in the tail of the Virginia opossum (Didelphis virginiana). Anat Rec (Hoboken). 2013;296:96-107 pubmed publisher
Curry J, Hohl R, Noakes T, Kohn T. High oxidative capacity and type IIx fibre content in springbok and fallow deer skeletal muscle suggest fast sprinters with a resistance to fatigue. J Exp Biol. 2012;215:3997-4005 pubmed publisher
Park K, Lim J, Sohn S, Oh S. Myosin heavy chain isoform expression in human extraocular muscles: longitudinal variation and patterns of expression in global and orbital layers. Muscle Nerve. 2012;45:713-20 pubmed publisher
Douillard A, Galbes O, Begue G, Rossano B, Levin J, Vernus B, et al. Calpastatin overexpression in the skeletal muscle of mice prevents clenbuterol-induced muscle hypertrophy and phenotypic shift. Clin Exp Pharmacol Physiol. 2012;39:364-72 pubmed publisher
Pearen M, Eriksson N, Fitzsimmons R, Goode J, Martel N, Andrikopoulos S, et al. The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue. Mol Endocrinol. 2012;26:372-84 pubmed publisher
Lee Y, Mikesh M, Smith I, Rimer M, Thompson W. Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons. Dev Biol. 2011;356:432-44 pubmed publisher
Kohn T, Burroughs R, Hartman M, Noakes T. Fiber type and metabolic characteristics of lion (Panthera leo), caracal (Caracal caracal) and human skeletal muscle. Comp Biochem Physiol A Mol Integr Physiol. 2011;159:125-33 pubmed publisher
Frese S, Velders M, Schleipen B, Schanzer W, Bloch W, Diel P. Myosin heavy chain expression pattern as a marker for anabolic potency: desoxymethyltestosterone (madol), norandrostenedione and testosterone repress MHC-IIb expression and stimulate MHC-IId/x expression in orchiectomized rat gastrocnemius muscle. Arch Toxicol. 2011;85:635-43 pubmed publisher
Rhee H, Hoh J. Immunohistochemical analysis of the effects of cross-innervation of murine thyroarytenoid and sternohyoid muscles. J Histochem Cytochem. 2010;58:1057-65 pubmed publisher
Moncman C, Andrade F. Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles. Exp Cell Res. 2010;316:1958-65 pubmed publisher
Yin X, Cuello F, Mayr U, Hao Z, Hornshaw M, Ehler E, et al. Proteomics analysis of the cardiac myofilament subproteome reveals dynamic alterations in phosphatase subunit distribution. Mol Cell Proteomics. 2010;9:497-509 pubmed publisher
Zhong W, Withers K, Hoh J. Effects of hypothyroidism on myosin heavy chain composition and fibre types of fast skeletal muscles in a small marsupial, Antechinus flavipes. J Comp Physiol B. 2010;180:531-44 pubmed publisher
Hyatt J, Roy R, Rugg S, Talmadge R. Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles. J Exp Zool A Ecol Genet Physiol. 2010;313:45-57 pubmed publisher
Stirn Kranjc B, Smerdu V, Erzen I. Histochemical and immunohistochemical profile of human and rat ocular medial rectus muscles. Graefes Arch Clin Exp Ophthalmol. 2009;247:1505-15 pubmed publisher
Rhee H, Steel C, Derksen F, Robinson N, Hoh J. Immunohistochemical analysis of laryngeal muscles in normal horses and horses with subclinical recurrent laryngeal neuropathy. J Histochem Cytochem. 2009;57:787-800 pubmed publisher
Hoh J, Li Z, Qin H, Hsu M, Rossmanith G. Cross-bridge kinetics of fast and slow fibres of cat jaw and limb muscles: correlations with myosin subunit composition. J Muscle Res Cell Motil. 2007;28:329-41 pubmed publisher
Zhong W, Lucas C, Hoh J. Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion. J Comp Physiol B. 2008;178:47-55 pubmed
Li Z, Lehar M, Nakagawa H, Hoh J, Flint P. Differential expression of myosin heavy chain isoforms between abductor and adductor muscles in the human larynx. Otolaryngol Head Neck Surg. 2004;130:217-22 pubmed
Zhong W, Lucas C, Kang L, Hoh J. Electrophoretic and immunochemical evidence showing that marsupial limb muscles express the same fast and slow myosin heavy chains as eutherians. Electrophoresis. 2001;22:1016-20 pubmed
Lucas C, Kang L, Hoh J. Monospecific antibodies against the three mammalian fast limb myosin heavy chains. Biochem Biophys Res Commun. 2000;272:303-8 pubmed
product information
Internal ID :
3291
Name :
10F5
Depositor Name :
Lucas, C.
Depositor Institution :
University of Sydney
Date Deposited :
9/26/07
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
myosin heavy chain, fast, 2B
Antigen Species :
Rabbit
Host Species :
mouse
Isotype :
MIgM, kappa light chain
Isotype for catalog (legacy) :
IgM, kappa light chain
Positive Tested Species Reactivity :
Antechinus,Bandicoot,Bettongia,Cheetah,Guinea Pig,Human,Kangaroo,Mouse,Porcine,Pseudocheirus,Rabbit,Rat,Trichosurus,Wallaby
Species Tested (legacy) :
guinea pig, rat, mouse, rabbit, horse and cat
Initial Publication Pubmed ID :
10872844
Collections :
Cytoskeleton,Human,Skeletal muscle
Search Keywords :
Lucas, myosin heavy chain, fast, 2B, mouse, MYH4, MHC, monoclonal, AB_1157896, IgM, Antechinus,Bandicoot,Bettongia,Cheetah,Guinea Pig,Human,Kangaroo,Mouse,Porcine,Pseudocheirus,Rabbit,Rat,Trichosurus,Wallaby, Immunofluorescence,Immunohistochemistry,Western Blot
Antigen Molecular Weight :
Predicted: 223kDa
Gene :
MYH4
Alternate Gene Name(s) :
MYH1, MHC
Uniprot ID :
Q28641
Antibody Registry ID :
AB_1157896
Immunogen :
rabbit retractor bulbi muscle
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
10872844 19609551 22499099 23451164 19768738 23989883 23504871 11358122 17703312 22300302 20713983 20012435
Immunofluorescence Pubmed IDs :
20037178 20350540 23835334 24522207 23319059 21658376 22282471
Western Blot Pubmed IDs :
10872844 14990919 23463416 20957349 11358122 17703312
Pubmed IDs :
23152195 21320626 19398607 22899533 18320334
Depositor Growth Medium :
Iscove's
DSHB Growth Medium :
Iscove's
References (legacy) :
Biochem. Biophys. Res. Commun. 272, 303-308.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US