product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
ecdysone receptor (EcR) (common), Manduca sexta
catalog :
10F1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10f1
reactivity :
squinting bush brown, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 6
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; squinting bush brown; 1:5; fig 4
Monteiro A, Tong X, Bear A, Liew S, Bhardwaj S, Wasik B, et al. Differential Expression of Ecdysone Receptor Leads to Variation in Phenotypic Plasticity across Serial Homologs. PLoS Genet. 2015;11:e1005529 pubmed publisher
Rajagopalan V, Zucker I, Jones J, Carlson M, MA Y. Cardiac ErbB-1/ErbB-2 mutant expression in young adult mice leads to cardiac dysfunction. Am J Physiol Heart Circ Physiol. 2008;295:H543-54 pubmed publisher
Vafopoulou X, Steel C, Terry K. Edysteroid receptor (EcR) shows marked differences in temporal patterns between tissues during larval-adult development in Rhodnius prolixus: correlations with haemolymph ecdysteroid titres. J Insect Physiol. 2005;51:27-38 pubmed
Li B, Yang Z, Hou J, McCracken A, Jennings M, Ma M. Compromised reproductive function in adult female mice selectively expressing mutant ErbB-1 tyrosine kinase receptors in astroglia. Mol Endocrinol. 2003;17:2365-76 pubmed
Ghbeish N, Tsai C, Schubiger M, Zhou J, Evans R, McKeown M. The dual role of ultraspiracle, the Drosophila retinoid X receptor, in the ecdysone response. Proc Natl Acad Sci U S A. 2001;98:3867-72 pubmed
Jindra M, Malone F, Hiruma K, Riddiford L. Developmental profiles and ecdysteroid regulation of the mRNAs for two ecdysone receptor isoforms in the epidermis and wings of the tobacco hornworm, Manduca sexta. Dev Biol. 1996;180:258-72 pubmed
product information
Internal ID :
1511
Name :
10F1
Depositor Name :
Riddiford, L.M.
Depositor Institution :
Janelia Farm Research Campus
Date Deposited :
2/11/98
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
ecdysone receptor (EcR) (common), Manduca sexta
Antigen Species :
Manduca sexta
Host Species :
mouse
Isotype :
MIgG2b, kappa light chain
Isotype for catalog (legacy) :
IgG2b, kappa light chain
Positive Tested Species Reactivity :
Bombyx mori,Drosophila,Locusta migratoria,Manduca,Rhodnius prolixus
Species Tested (legacy) :
Manduca; Bombyx mori; Locusta migratoria; Drosophila melanogaster
Initial Publication Pubmed ID :
8948589
Depositor Notes (Special Instructions) :
10F1 is used in the ecodysone-induced gene expression system
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Riddiford, ecdysone receptor, EcR, EcR, Bombyx mori, Drosophila, Locusta , migratoria, Manduca, Rhodnius prolixus, NR1H1, monoclonal, AB_528210, IgG2b, Immunofluorescence,Immunohistochemistry,Western Blot
Antigen Molecular Weight :
Predicted: 62.8kDa; Apparent: 70-80kDa
Gene :
EcR
Alternate Gene Name(s) :
NR1H1
Uniprot ID :
P49883
Antibody Registry ID :
AB_528210
Immunogen :
E. coli: fusion protein of EcR-B1 (amino acids 33-454) fused to glutathione-S-transferase; only the EcR-B1 portion of the fusion protein injected
Immunogen Sequence :
amino acids 33-454 of EcR-B1
Clonality :
Monoclonal
Myeloma Strain :
Fox-NY
Epitope Mapped :
Yes
Epitope Location or Sequence :
amino acids 127-354 of EcR-B1
Epitope Map PubMed ID :
8948589
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
8948589
Immunofluorescence Pubmed IDs :
11274407 15686643
Western Blot Pubmed IDs :
8948589 15686643 15686643 12907756
Depositor Growth Medium :
Iscove's
DSHB Growth Medium :
Iscove's
References (legacy) :
Insect Biochem. Mol. Biol. 25, 845-856.; Dev. Biol. 180, 258-272.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US