product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
integrin alphaIIb
catalog :
10E5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10E5
reactivity :
human
application :
ELISA, immunoprecipitation, FACS
citations: 10
Reference
Buitrago L, Rendon A, Liang Y, Simeoni I, Negri A, Filizola M, et al. αIIbβ3 variants defined by next-generation sequencing: predicting variants likely to cause Glanzmann thrombasthenia. Proc Natl Acad Sci U S A. 2015;112:E1898-907 pubmed publisher
Zhu J, Choi W, McCoy J, Negri A, Zhu J, Naini S, et al. Structure-guided design of a high-affinity platelet integrin ?IIb?3 receptor antagonist that disrupts Mg²? binding to the MIDAS. Sci Transl Med. 2012;4:125ra32 pubmed publisher
Xiao T, Takagi J, Coller B, Wang J, Springer T. Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics. Nature. 2004;432:59-67 pubmed
Artoni A, Li J, Mitchell B, Ruan J, Takagi J, Springer T, et al. Integrin beta3 regions controlling binding of murine mAb 7E3: implications for the mechanism of integrin alphaIIbbeta3 activation. Proc Natl Acad Sci U S A. 2004;101:13114-20 pubmed
Mekrache M, Kieffer N, Baruch D. Activation of recombinant alphaIIbbeta3 expressed in Chinese hamster ovary cells exposes different binding sites for fibrinogen or von Willebrand factor: evidence using monoclonal antibodies to alphaIIbbeta3. Br J Haematol. 2002;116:636-44 pubmed
Chen P, Melchior C, Brons N, Schlegel N, Caen J, Kieffer N. Probing conformational changes in the I-like domain and the cysteine-rich repeat of human beta 3 integrins following disulfide bond disruption by cysteine mutations: identification of cysteine 598 involved in alphaIIbbeta3 activation. J Biol Chem. 2001;276:38628-35 pubmed
Kaul D, Tsai H, Liu X, Nakada M, Nagel R, Coller B. Monoclonal antibodies to alphaVbeta3 (7E3 and LM609) inhibit sickle red blood cell-endothelium interactions induced by platelet-activating factor. Blood. 2000;95:368-74 pubmed
Depraetere H, Ajzenberg N, Girma J, Lacombe C, Meyer D, Deckmyn H, et al. Platelet aggregation induced by a monoclonal antibody to the A1 domain of von Willebrand factor. Blood. 1998;91:3792-9 pubmed
Coller B, Peerschke E, Scudder L, Sullivan C. A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa. J Clin Invest. 1983;72:325-38 pubmed
Coller B, Beer J, Scudder L, Steinberg M. Collagen-platelet interactions: evidence for a direct interaction of collagen with platelet GPIa/IIa and an indirect interaction with platelet GPIIb/IIIa mediated by adhesive proteins. Blood. 1989;74:182-92 pubmed
product information
Internal ID :
8416
Name :
10E5
Depositor Name :
Coller, B.S.
Depositor Institution :
Laboratory of Blood and Vascular Biology, Rockefeller University
Date Deposited :
3/3/12
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
integrin alphaIIb
Antigen Species :
human
Host Species :
mouse
Isotype :
MIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Human,Primate
Species Tested (legacy) :
human and primates
Initial Publication Pubmed ID :
6308050
Depositor Notes (Special Instructions) :
Inhibits ligand (fibrinogen-von Willebrand factor) binding to integrin alphaIIbbeta3 and platelet aggregation; does not react with platelets of patients with Glanzmann thrombasthenia. This antibody doesn't appear to work for western blotting.
Collections :
CD antigens,Cell adhesion,Cell signaling,Human
Search Keywords :
Coller, B.S., integrin alphaIIb, ITGA2B, CD41, GPIIb, GT, GTA, HPA3, CD41B, ITGAB, monoclonal, human, AB_2617389, IgG2a, Primate, ELISA,FACS,Function Blocking,Immunoprecipitation
Antigen Molecular Weight :
Predicted: 103, 110, and 113 kDa; Apparent: 140 kDa
Gene :
ITGA2B
Alternate Gene Name(s) :
CD41, GPIIb, GT, GTA, HPA3, CD41B, ITGAB
Uniprot ID :
P17301
Antibody Registry ID :
AB_2617389
Immunogen :
Human platelets
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
X63-Ag8.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
alphaIIb cap subdomain
Epitope Map PubMed ID :
15378069
Recommended Applications :
ELISA,FACS,Function Blocking,Immunoprecipitation
Immunoblotting (legacy) :
no
Immunoprecipitation Pubmed IDs :
6308050 15277669
Function Blocking Pubmed IDs :
6308050 2546619 22422993 9573016 11849224 15277669
FACS Pubmed IDs :
2546619 15277669 25827233
Pubmed IDs :
10627437 11507099
Depositor Growth Medium :
DMEM – 10% FBS
Additional Information :
10E5 recognizes the alphaIIb in its native conformation associated to beta3 integrin.
DSHB Growth Medium :
Iscove's
References (legacy) :
Blood 61, 99-110.; J. Clin. Invest. 72, 325-338.; Blood 95, 368-374.; J. Biol. Chem. 276, 38628-38635.; Br. J. Haematol. 116, 636-644.; Nature 432, 59-67.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US