product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Germ cell nuclear antigen 1; GCNA1
catalog :
10D9G11
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
10D9G11
reactivity :
mouse
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 14
Reference
Carmell M, Dokshin G, Skaletsky H, Hu Y, Van Wolfswinkel J, Igarashi K, et al. A widely employed germ cell marker is an ancient disordered protein with reproductive functions in diverse eukaryotes. elife. 2016;5: pubmed publisher
Itman C, Bielanowicz A, Goh H, Lee Q, Fulcher A, Moody S, et al. Murine Inhibin α-Subunit Haploinsufficiency Causes Transient Abnormalities in Prepubertal Testis Development Followed by Adult Testicular Decline. Endocrinology. 2015;156:2254-68 pubmed publisher
Moody S, Goh H, Bielanowicz A, Rippon P, Loveland K, Itman C. Prepubertal mouse testis growth and maturation and androgen production are acutely sensitive to di-n-butyl phthalate. Endocrinology. 2013;154:3460-75 pubmed publisher
Holloway J, Mohan S, Balmus G, Sun X, Modzelewski A, Borst P, et al. Mammalian BTBD12 (SLX4) protects against genomic instability during mammalian spermatogenesis. PLoS Genet. 2011;7:e1002094 pubmed publisher
Brown J, Bullwinkel J, Baron Luhr B, Billur M, Schneider P, Winking H, et al. HP1gamma function is required for male germ cell survival and spermatogenesis. Epigenetics Chromatin. 2010;3:9 pubmed publisher
Bristol Gould S, Kreeger P, Selkirk C, Kilen S, Cook R, Kipp J, et al. Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool. Dev Biol. 2006;298:132-48 pubmed
Maatouk D, Kellam L, Mann M, Lei H, Li E, Bartolomei M, et al. DNA methylation is a primary mechanism for silencing postmigratory primordial germ cell genes in both germ cell and somatic cell lineages. Development. 2006;133:3411-8 pubmed
Tay J, Hodgman R, Sarkissian M, Richter J. Regulated CPEB phosphorylation during meiotic progression suggests a mechanism for temporal control of maternal mRNA translation. Genes Dev. 2003;17:1457-62 pubmed
Tay J, Richter J. Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice. Dev Cell. 2001;1:201-13 pubmed
Rao D, Chang J, Kumar P, Mizukami I, Smithson G, Bradley S, et al. Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors. Mol Cell Biol. 2001;21:7796-806 pubmed
Enders G, May J. Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice. Dev Biol. 1994;163:331-40 pubmed
Hayward R, White J, Ead H, Baldwin A, Swanton H. A microcomputer based catheter laboratory system for analysis of coronary blood flow in man. J Biomed Eng. 1983;5:248-52 pubmed
Goyal R, Sharma R, Mathur G, Andleigh H. Pathogenicity of different strains of vaccinia virus. Indian J Med Sci. 1969;23:247-51 pubmed
Eddy C, Keshav K, An H, Utt E, Mejia J, Ingram L. Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon. J Bacteriol. 1991;173:245-54 pubmed
product information
Internal ID :
24600
Name :
10D9G11
Depositor Name :
Enders, George
Depositor Institution :
University of Kansas Medical School
Date Deposited :
12/14/16
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Germ cell nuclear antigen 1; GCNA1
Antigen Species :
Mouse
Host Species :
rat
Isotype :
RIgM
Positive Tested Species Reactivity :
Mouse,Rat
Initial Publication Pubmed ID :
8200475
Depositor Notes (Special Instructions) :
Over 100 publications have used this antibody to recognize germ cells. The protein target has recently be identified as the Gcna gene product. See PMID: 27718356
Collections :
Cell markers
Search Keywords :
Enders, George, Germ cell nuclear antigen 1; GCNA1, Mouse, RIgM, Mouse/Rat, Gcna, Gcna1, AB_2629436, Monoclonal, Cell Markers, Immunohistochemistry/Immunofluorescence/Western blot/FFPE
Antigen Molecular Weight :
80-110 kDa depending upon modification
Gene :
Gcna
Alternate Gene Name(s) :
Gcna1
Antibody Registry ID :
AB_2629436
Immunogen :
Pachytene spermatocytes
Clonality :
Monoclonal
Myeloma Strain :
SP2/N
Epitope Mapped :
No
Epitope Location or Sequence :
8-amino-acid tandem GE(P/M/S)E(S/T)EAK repeat
Epitope Map PubMed ID :
27718356
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
20423503 11702780 21655083 8200475 16930587 25781564 11604514 23766129 16887828 21655083
Immunofluorescence Pubmed IDs :
21655083 8200475 12815066
Western Blot Pubmed IDs :
8200475
FFPE Pubmed IDs :
20423503 1702780 16930587 5781564 11604514 6887828 21655083
Additional Information :
The epitope is sensitive to glutarldehyde and trypsin. RRID:AB_2629436
DSHB Growth Medium :
Iscove's
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US